Literature DB >> 3348775

Expression of erythroid differentiation factor (EDF) in Chinese hamster ovary cells.

M Murata1, K Onomichi, Y Eto, H Shibai, M Muramatsu.   

Abstract

Plasmid DNA containing EDF subunit cDNA and mouse dihydrofolate reductase (DHFR) cDNA was transfected into CHO DHFR- cells by the calcium-phosphate method. DHFR positive transformants secreted recombinant EDF (r-EDF) constitutively in an active form and accumulated it in the conditioned medium. Furthermore, cells which were resistant to methotrexate (MTX : 0.5 microM) secreted r-EDF up to 1 microgram/ml. r-EDF was identical to natural EDF (n-EDF) produced by human acute monocytic leukemia cell line, THP-1, as regards its dimeric structure and a biological activity.

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Year:  1988        PMID： 3348775     DOI： 10.1016/0006-291x(88)90583-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

Review 1.  The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation.

Authors:  J Auwerx
Journal:  Experientia       Date:  1991-01-15

2.  Expression of immunoreactive activin A protein in remodeling lesions associated with interstitial pulmonary fibrosis.

Authors:  T Matsuse; A Ikegami; E Ohga; T Hosoi; T Oka; K Kida; M Fukayama; S Inoue; T Nagase; Y Ouchi; Y Fukuchi
Journal:  Am J Pathol       Date:  1996-03       Impact factor: 4.307

3.  Activin A inhibits Pax-6 expression and perturbs cell differentiation in the developing spinal cord in vitro.

Authors:  F Pituello; G Yamada; P Gruss
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-18       Impact factor: 11.205

4.  A new bacterial cellulose substrate for mammalian cell culture. A new bacterial cellulose substrate.

Authors:  K Watanabe; Y Eto; S Takano; S Nakamori; H Shibai; S Yamanaka
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

5.  Activin A is stimulated by tumor necrosis factor-alpha and modulates collagen gene expression in human amniotic cells.

Authors:  Y Abe; M Komatsubara; M Saito; M Toda; H Shinozaki; T Tamura; Y Kasahara; H Sedakata; T Minegishi
Journal:  J Endocrinol Invest       Date:  2013-02-04       Impact factor: 4.256

6.  Evidence for the participation of endogenous activin A/erythroid differentiation factor in the regulation of erythropoiesis.

Authors:  M Shiozaki; R Sakai; M Tabuchi; T Nakamura; K Sugino; H Sugino; Y Eto
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

7.  Receptor oligomerization and beyond: a case study in bone morphogenetic proteins.

Authors:  Kai Heinecke; Axel Seher; Werner Schmitz; Thomas D Mueller; Walter Sebald; Joachim Nickel
Journal:  BMC Biol       Date:  2009-09-07       Impact factor: 7.431

8.  Activin A: an autocrine inhibitor of initiation of DNA synthesis in rat hepatocytes.

Authors:  H Yasuda; T Mine; H Shibata; Y Eto; Y Hasegawa; T Takeuchi; S Asano; I Kojima
Journal:  J Clin Invest       Date:  1993-09       Impact factor: 14.808

9.  Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells.

Authors:  H Mashima; H Ohnishi; K Wakabayashi; T Mine; J Miyagawa; T Hanafusa; M Seno; H Yamada; I Kojima
Journal:  J Clin Invest       Date:  1996-04-01       Impact factor: 14.808

10.  Activin a in the regulation of corneal neovascularization and vascular endothelial growth factor expression.

Authors:  Vassiliki Poulaki; Nicholas Mitsiades; Friedrich E Kruse; Sven Radetzky; Eirini Iliaki; Bernd Kirchhof; Antonia M Joussen
Journal:  Am J Pathol       Date:  2004-04       Impact factor: 4.307
  10 in total

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