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Literature DB >> 33484484

Growth and Maintenance of Escherichia coli Laboratory Strains.

Amie R Tuttle¹, Nicholas D Trahan¹, Mike S Son¹.

Abstract

Escherichia coli is a Gram-negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching- or research-associated laboratory.

© 2021 Wiley Periodicals LLC. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture. © 2021 Wiley Periodicals LLC.

Entities: Chemical

Keywords: Escherichia coli; growth; maintenance

Mesh：

Substances：
Culture Media
Agar

Year: 2021 PMID： 33484484 PMCID： PMC8006063 DOI： 10.1002/cpz1.20

Source DB: PubMed Journal: Curr Protoc ISSN： 2691-1299

14 in total

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Authors: Y Komeda; T Iino
Journal: J Bacteriol Date: 1979-09 Impact factor: 3.490

2. Definition of the Escherichia coli MC4100 genome by use of a DNA array.

Authors: Joseph E Peters; Timothy E Thate; Nancy L Craig
Journal: J Bacteriol Date: 2003-03 Impact factor: 3.490

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

Authors: S Lacks; B Greenberg
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

4. Growth of Escherichia coli at elevated temperatures.

Authors: Upinder Fotadar; Philip Zaveloff; Louis Terracio
Journal: J Basic Microbiol Date: 2005 Impact factor: 2.281

5. DNA restriction enzyme from E. coli.

Authors: M Meselson; R Yuan
Journal: Nature Date: 1968-03-23 Impact factor: 49.962

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

7. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

Authors: M J Casadaban
Journal: J Mol Biol Date: 1976-07-05 Impact factor: 5.469

Growth and Maintenance of Escherichia coli Laboratory Strains.

1. Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions.

2. Definition of the Escherichia coli MC4100 genome by use of a DNA array.

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

4. Growth of Escherichia coli at elevated temperatures.

5. DNA restriction enzyme from E. coli.

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

7. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

8. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

9. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

10. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.

1. Preparation and Use of Cellular Reagents: A Low-resource Molecular Biology Reagent Platform.