Response to reduced dietary protein level on growth performance in growing Windsnyer pigs.

The objective of the study was to determine the growth performance response to reduced dietary protein level and to identify the optimum level of protein for the performance in growing Windsnyer pigs. Maize-soybean-based diets were formulated to contain a protein level of 193 g/kg, 173.7 g/kg, 154.4 g/kg, 135.1 g/kg, 115.8 g/kg and 96.5 g/kg. The 193 g/kg (control diet) is the standard recommended by the National Research Council. The amino acid levels and net energy value were similar in all treatments. The study was conducted with five growing Windsnyer pigs per each protein inclusion level. The trial was carried out with individually caged pigs aged 4 months, weighing about 23 (SD = 1.39) kg. The experiment lasted a period of 8 weeks excluding 2 weeks of the adaptation period. The feed and water were offered ad libitum. The average daily feed intake (ADFI), average daily gain (ADG), feed conversion ratio (FCR), final body weight (FBW) and metabolic body weight (BW0.75) were measured. Polynomial regression and piecewise regressions were used to analyse data. Decremental levels of protein did not affect (p > 0.05) ADFI and ADG. Reduced levels of protein influenced FBW, BW0.75 and FCR (p < 0.05). A decreasing quadratic response was observed in FCR, as CP decreased from 193 g/kg to 135.1, FCR improved from 3.18 to 2.13 then suddenly decreased with the CP level. There was a quadratic increase in BW0.75 and FBW as inclusion levels of protein were reduced (p < 0.05). They increased from 193 g/kg (FBW 39.5 and BW0.75 12.8) protein level until 135.1 g/kg (FBW 45.1 and BW0.75 14.6) then begun to decrease as dietary protein level was decreased further. Using stepwise piece (broken stick) methods, the optimum protein level for FCR was estimated to be at 135.1 g/kg (p < 0.05). The maximum level of dietary protein for BW and BW0.75 was obtained at 135.1 g/kg. Reduction of the CP level in the Windsnyer pig diet beyond 138.3, 132.1 and 132.3 g/kg hinders the FCR, FBW and BW0.75, respectively. Protein levels can be reduced from the standard recommendation level of 193 g/kg to 135.1 g/kg without compromising the growth performance of growing Windsnyer pigs.

Introduction

The commercial value of indigenous pigs to improve pig production has been gaining impetus (Nistor et al., 2012; Hlatini et al., 2020; Huang et al., 2020; Kasprzyk and Bogucka, 2020). This is due to the high demand for pork because of the ever-increasing human population coupled with climate change and pandemic diseases such as existing COVID-19. The demand for indigenous pork for fresh consumption is increasing. The demand for pork from indigenous pigs is also driven by the taste and preferences of the consumers. Thus, research that prioritizes indigenous pigs is relevant.

Indigenous pigs have the ability to produce under marginal nutrient availability. Indigenous pigs have also demonstrated the ability to utilize high fibrous diets due to their unique microbial community (Kanengoni et al., 2014; Ncobela et al., 2018). Indigenous pigs are commonly fed with energy-rich feedstuffs such as tree leaves, grasses, kitchen waste, maize cobs and local fruit, which are mostly enough for maintenance and low production. The low-quality protein in the diet fed to indigenous pigs could cause poor production performance. These energy-rich diets have either low protein levels or inadequate protein for utilization.

Protein, which is an indispensable nutrient, supplies amino acids required for growth performance and production of pigs (Whittemore et al., 2001). An attempt to reduce protein content in the diet of pigs is a contemporary subject. The protein content is reduced to save the in-demand protein-rich ingredients and reduce feed cost for pigs (Wang et al., 2018). Reduction in the inclusion of protein in the diet of pigs reduces nitrogen in the excreta (Hlatini et al., 2020), which contributes to the emission of ammonia and nitrite gases (Hansen et al., 2014). High excreta nitrogen induces acidification and eutrophication of ecosystems and odour emissions (Mpendulo et al., 2018; Wang et al., 2018). Low-protein diets maintain gut health and positively influence intestinal morphology and microbiota (Wang et al., 2018).

There are several studies done aiming at reducing protein levels in the diet of fast-growing and slow-growing pigs (Noblet et al., 2001; Heo et al., 2008; Gallo et al., 2014; Hansen et al., 2014; He et al., 2016; Wang et al., 2018). However, very few, if any, have attempted to precisely determine protein needs for indigenous pigs using piecewise regression. Inadequate supply of protein in the diet of pigs compromises protein synthesis and subsequently growth performance (Whittemore et al., 2001). On the other hand, supplying excess dietary protein fuels high protein turnover, muscle respiration and visceral organ mass (Noblet et al., 2001; Gallo et al., 2014; Toledo et al., 2014). Therefore, there is a need to accurately determine protein needs for indigenous Windsnyer without compromising growth performance.

Knowledge on the optimum protein requirements for indigenous pigs optimizes protein efficiency and guides feed formulators with protein requirements for Windsnyer pigs. The indigenous growing Moo Lath pigs required about 150 g/kg of crude protein in the diet (Phengsavanh and Lindberg, 2013). The indigenous Mong Cai pigs showed a great performance on the diet with 130 to 140 dietary protein levels (Ly et al., 2003; Pham et al., 2010); similar findings were obtained in Cinta Senese pigs of Italy (Sirtori et al., 2010). Indigenous Windsnyer pigs might require less protein level than NRC (2012)-estimated protein intake for leaner and fast-growing pigs. The optimal protein level for indigenous Windsnyer pigs has not been defined. This necessitates research to determine the growth performance of the indigenous Windsnyer pigs given a diet that contains decreasing protein levels. The objective of the study was, therefore, to determine the response in the growth performance to reduced dietary levels in growing Windsnyer pigs. It was hypothesized that reduction of dietary protein level beyond optimum levels would compromise growth performance in indigenous Windsnyer pigs.

Materials and methods

Study site description and ethical clearance

The study was then conducted at the Agricultural Research Council (ARC), Animal Production Institute, Irene, South Africa. The average annual temperature during the period of the study was 20.7 °C. The care and use of experimental animals were according to the approved standards by the Animal Ethics Committee of the Agricultural Research Council-Animal Production Institution (reference number APIEC 17/12).

Pig management

Thirty clinically healthy male slow-growing Windsnyer pigs of about 4 months of age, with an initial body weight of about 23 (SD = 1.39) kg, were randomly allocated into individual pens measuring 1.5 × 1 m2. The pigs were ear-tagged and housed in a barn with artificial lighting and a proper ventilation system. The house temperature and humidity were maintained at about 21.9 ± 2.24 °C and 45.2 ± 6.85%, respectively. A 12-h dark-12-h artificial light cycle was applied. Each pen was provided with water through a low-pressure nipple drinker and feed was supplied in a self-feeder trough. Feed and water were offered ad libitum. The pigs received no antibiotics or growth promoters.

Experimental diet and design

The control diet contained 193 g/kg protein level that was formulated according to the standards for the trial pig age recommended by NRC (2012). Then, the protein level was reduced at the expense of soybean meal to reach the desired low-protein diets containing 173.7, 154.4, 135.1, 115.8 and 96.5 g/kg CP but with levels of essential amino acids the same as in the control. The six diets were formulated to have about 9.5 MJ in all diets. Low-protein diets were formulated by reducing the CP level through partial substitution of soybean meal by yellow maize and wheat bran. The experimental diets were formulated to meet and exceed NRC requirements for all other nutrients for growing pigs (NRC, 2012). Diets were formulated using the software program Bestmix® (Adifo, Belgium). Experimental diets were allocated randomly to pens and each pig was treated as an experimental unit, in a complete randomized design. There were five pigs for each dietary treatment. Before the commencement of data collection, pigs were allowed an adaptation period of 14 days to the environment and experimental diets. Then, Windsnyer pigs were fed experimental diets in mash form for 8 weeks.

Chemical analyses

Representative diet samples were taken from all the feeders per dietary treatment 3 days after the beginning and 3 days before the end of the experiment. They were kept at a cool dry place until they were homogenized, subsampled and prepared for use for chemical composition analysis. All diet samples were analysed in triplicate (Table 1). Proximate analyses were determined using the Association of Official Analytical Chemists (AOAC, 1990). Dry matter, ash, crude protein and ether extract were analysed according to official methods, viz. 945.15, 942.05, 979.09 and 920.39, respectively. Nitrogen content was determined using the Dumas combustion method in a LECO TruSpec nitrogen analyser (St. Joseph, MI, USA). Crude protein was then calculated as N × 6.25. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) levels were analysed using a ANKOM fibre analyser (ANKOM Macedon, NY, USA), according to Van Soest et al. (1991). Digestible energy (DE) was calculated from the equation adapted from McDonald et al. (2010). Digestible energy (MJ/kg) = 17.47 + (0.0076 × CP g/kg) + (0.0158 × acid hydrolysed ether extract g/kg) − (0.0331 × ash g/kg) − (0.0140 × NDF g/kg).

Table 1

Ingredient and chemical composition of diets used

Ingredients (g/kg)	Decreasing levels of crude protein (g/kg DM)
	193	173.7	154.4	135.1	115.8	96.5
Yellow maize	602	633	688	725	767	720
Soya oil cake	273	208	171	117	750	50
Wheat bran	85	119	100	120	120	155
Limestone	13	12.5	12.1	13	13.5	10
Monocalcium phosphate	6.0	6.0	4.0	4.0	4.0	5.0
Oil-sunflower	5.0	5.0	5.0	0.5	5.0	5.0
Salt	5.0	5.0	5.0	5.0	5.0	5.0
Lysine HCL	3.8	3.8	3.8	3.8	3.8	3.0
Threonine	1.1	1.1	1.1	1.1	1.1	1.1
Methionine	1.0	1.0	1.0	1.0	1.0	0.95
Synth. tryptophan	0.2	0.2	0.2	0.2	0.2	0.2
Vitamin-mineral premix	4.7	4.7	4.7	4.8	4.7	4.9
Chemical analysis
Dry matter (g/kg)	882	882	876	875	875	876
Calculated DE (MJ/kg DM)	15.9	15.2	15.5	15.6	14.5	14.6
Crude protein (g/kg DM)	202	177	161	145	121	103
Ether extract (g/kg DM)	27.2	21.7	22.0	31.2	27.2	24.1
Neutral detergent fibre (g/kg DM)	142	174	158	173	200	197
Acid detergent fibre (g/kg DM)	44.2	50.5	47.2	47.8	45.3	46.5
Ash (g/kg DM)	46.7	46.2	40.6	30.6	45.0	39.4
Calculated nutrient analysis
Dry matter (g/kg)	884	883	882	880	879	880
Net energy value (MJ/kg DM)	9.76	9.78	9.92	10.1	10.1	9.99
Crude protein (g/kg DM)	192	169	153	137	117	100
Ether extract (g/kg DM)	37.2	38.0	38.2	39.0	39.4	44.1
Crude fibre (g/kg DM)	27.3	28.6	25.9	26.4	25.3	27.3
Ash (g/kg DM)	54.8	52.8	50.3	46.5	45.0	55.2

Measurements

Average daily feed intake (ADFI), average daily gain (ADG), feed conversion ratio (FCR), metabolic body weight and final body weight (FBW) were measured. Average daily feed intake (ADFI) for each week was estimated by dividing feed consumed for that week by 7. Feed consumed was calculated as feed offered minus feed left and spillages. Plastic trays were placed underneath each pen to collect spillages. Average daily gain (ADG) was determined by dividing the difference between body weight at the beginning and the end of each week by 7. The FCR for each pig was also calculated by dividing ADFI with ADG. Metabolic body weight (BW0.75) was calculated as weight0.75. Weighing of pigs was done every week from 0700h to 0830h throughout the trial.

Statistical analyses

For the repeated measurements, such as ADFI, ADG, FCR and BW0.75 during growth, the SAS PROC MIXED (SAS, 2008) was used to test for significance of the level of crude protein and week on performance parameters. The first-order autoregressive correlation (AR [1]) was fitted to the model. The initial body weight of pigs was included in the model as a covariate. The regression model PROC REG (SAS, 2008) was used to determine the relationships between protein inclusion levels against ADFI, ADG, FCR, BW0.75 and FBW. Then, the CP criterion was used to determine a model maximizing protein level with as few variables as possible. The piecewise regression (broken stick) analysis using the PROC NLIN (SAS, 2008) procedure was used to determine the optimum inclusion levels of dietary protein on FCR, FBW and BW0.75. A level of p < 0.05 was set at the criterion for statistical significance. The piecewise regression model used was

Using parameters (yo, y1, y2) and the xc, the two segmented simple regression functions were

whereYi is the response variable when crude protein inclusion level is constraining performance; Yj is the response variable before the level of crude protein diet compromise performance; and Yk is the response variable when the level of crude protein in the diet exceeds the optimal level;

when xi = 0;

yo is the intercept or minimum Yi when xc < 0; y1 is the rate of change of Yi when xi < xc; y2 is the rate of increase in Yi when xi > xc; xi is the level of crude protein in the diet; xc is the optimal protein level beyond which performance is compromised by a decrease in the level of crude protein in the diets; and Ixc is a dummy variable with value 0 when xi < xc and 1 when xi ≥ xc. Measurements done were considered significant at p < 0.05.

Results

The significance levels of the effect of a diet with decreasing levels of dietary protein on growth performance in Windsnyer pigs are illustrated in Table 2. Dietary proein level affected body weight (BW), metabolic body weight (BW0.75), and average daily feed intake (ADFI) affected average daily gain (ADG) and feef conversion ration (FCR) (p < 0.01). The dietary protein level affected all measured parameters and their response that were different during the 8-week study period (p < 0.01). The dietary protein level and the week of feeding had a significant interaction on AFDI, ADG and FCR (p < 0.01) but did not affect BW and BW0.75 (p > 0.05).

Table 2

Significance levels (p value) for the effect of dietary protein level, week and their interaction on growth performance in slow-growing pigs

Parameter	Protein level	Week	Protein level × Week
Average daily feed intake	< .0001	< .0001	< .0001
Average daily gain	0.004	< .0001	< .0001
Feed conversion ratio	0.001	0.003	< .0001
Body weight (BW)	< .0001	< .0001	0.11
Metabolic body weight (BW0.75)	0.01	< .0001	0.22

The relationship between varying levels of CP and performance parameters is shown in Table 3. The relationship between dietary protein levels and ADFI was not significant (p > 0.05). Dietary protein levels were not related to ADG (p > 0.05). Feed conversion ratio exhibited a decreasing quadratic response against decremental levels of CP (p < 0.01). As levels of protein decreased, FCR decreased until reaching 135.1 g/kg and after that, it started to increase. There was an increasing quadratic relationship between dietary protein levels against FBW and BW0.75 (p < 0.01). As levels of protein decreased, FBW and BW0.75 increased until reaching 135.1 g/kg and thereafter, it started to decrease.

Table 3

Response of growth performance parameters to reduced dietary protein levels in growing Windsnyer pigs

Parameter	Dietary protein level as g/kg DM basis						MSE	p value
	193	173.7	154.4	135.1	115.8	96.5		Linear	Quadratic
ADFI (kg/day)	1.24 ± 0.036	1.34 ± 0.035	1.22 ± 0.033	1.12 ± 0.034	1.43 ± 0.035	1.13 ± 0.033	0.034	0.18	0.12
ADG (kg/day)	0.29 ± 0.017	0.34 ± 0.018	0.27 ± 0.018	0.27 ± 0.018	0.35 ± 0.019	0.24 ± 0.018	0.018	0.09	0.16
FCR	3.18 ± 0.322	3.06 ± 0.325	2.63 ± 0.326	2.13 ± 0.326	3.43 ± 0.328	4.77 ± 0.325	0.325	0.25	0.004
FBW (kg)	39.5 ± 1.286	40.6 ± 1.284	43.5 ± 1.285	45.1 ± 1.284	35.4 ± 1.285	34.7 ± 1.285	1.285	0.008	0.001
BW0.75 (kg)	12.8 ± 0.635	12.9 ± 0.636	14.1 ± 0.637	14.6 ± 0.636	11.8 ± 0.636	11.4 ± 0.636	0.636	0.002	0.001

MSE, standard error of the mean; FBW, body weight; BW, metabolic weight; ADFI, average daily feed intake; ADG, average daily gain; FCR, feed conversion ratio

The relationship between the week of feeding against ADFI, ADG, FCR and BW0.75 is shown in Table 4. There was a quadratic relationship between weeks of feeding and ADFI in pigs fed diet containing reduced protein level (p < 0.05). As the week progressed, ADFI increases until the 6th to the 7th week, and then it decreased. The quadratic response between ADG and weeks of feeding reduced dietary protein level was observed (p < 0.05). As the week progressed, ADG increased until the 5th week, and then it decreased afterwards. There was a decreasing quadratic relationship between weeks of feeding and FCR (p < 0.05). A positive linear relationship between progressive weeks of feeding experimental diet and BW0.75 was observed (p < 0.05).

Table 4

Least square means for growing Windsnyer pig performance fed on reduced protein levels in an 8-week period

Parameter	Weeks of feeding								MSE	p value
	1	2	3	4	5	6	7	8		Linear	Quadratic
ADF (kg/day)	1.11 ± 0.032	1.04 ± 0.032	1.10 ± 0.031	1.18 ± 0.031	1.36 ± 0.033	1.38 ± 0.033	1.38 ± 0.032	1.14 ± 0.032	0.032	0.03	0.002
ADG (kg/day)	0.23 ± 0.022	0.33 ± 0.023	0.36 ± 0.022	0.38 ± 0.022	0.39 ± 0.021	0.34 ± 0.022	0.31 ± 0.022	0.41 ± 0.022	0.022	< 0.001	< 0.001
FCR	2.43 ± 0.401	3.96 ± 0.401	2.89 ± 0.401	2.96 ± 0.401	3.91 ± 0.401	3.97 ± 0.401	3.89 ± 0.401	2.75 ± 0.401	0.401	0.02	0.003
BW0.75 (kg)	10.9 ± 0.278	11.6 ± 0.278	12.5 ± 0.278	13.1 ± 0.278	13.7 ± 0.278	14.4 ± 0.278	14.7 ± 0.278	15.8 ± 0.278	0.278	< 0.001	0.10

MSE, standard error of the mean; FBW, body weight; BW, metabolic weight; ADFI, average daily feed intake; ADG, average daily gain; FCR, feed conversion ratio

Optimum levels of protein in the diet without compromising growth performance were estimated using the broken stick analysis (Table 5). The optimum protein level for FCR was estimated at 138.3 g/kg protein level and the plateau was estimated at 2.70 (p < 0.05). For BW0.75 and FBW, the threshold of protein levels was estimated at 132.1 and 132.3 g/kg, respectively. The plateau of BW0.75 and FBW was estimated at 31.6 kg and 13.3 kg (p < 0.05), respectively.

Table 5

Optimal levels beyond which performance parameters in growing Windsnyer pigs is compromised by low levels of dietary protein

Y0			Y1		Y2		Xc	Plateau	p value
Parameter	Estimate	± SE	Estimate	± SE	Estimate	± SE
Feed conversion ratio	11.9	± 0.20	− 1.36	± 1.88	0.053	± 0.126	138.3	2.7	< 0.001
Final body weight (kg)	31.6	± 1.08	− 0.0087	± 0.52	− 0.051	± 0.052	132.1	31.6	0.001
Metabolic weight0.75	13.3	± 0.34	− 0.00044	± 0.17	− 0.016	± 0.017	132.3	13.3	0.003

SE, standard error; yo is the intercept or minimum Yi when xc < 0; y1 is the rate of change of Yi when xi < xc; y2 is the rate of increase in Yi when xi > xc; xi is the level of crude protein in the diet; xc is the optimal level beyond which performance is compromised by decrease in the level of crude protein in the diets

Discussion

No health problems occurred throughout the experimental period and no feed refusal was experienced. Decreasing protein levels were formulated by increasing fibre source such as white bran at the expense of soybean meal to reach the desired low levels of dietary protein. Windsnyer pigs have the ability to effectively utilize high fibre by virtue of specialized microbial community in the colon (Kanengoni et al., 2014; Ncobela et al., 2018).

The absence of a relationship between ADFI and protein levels is similar to the findings obtained by Noblet et al. (2001) who reported no effect of dietary protein levels on feed intake of growing pigs. Likewise, Gloaguen et al. (2014) and Toledo et al. (2014) observed no influence of reduced dietary protein on feed intake. No effect of protein levels to feed intake may suggest that nutrient density across diet was similar. The non-significant relationship between decremental levels of protein and ADG concurs with findings reported by Fuller et al. (1995) and Campbell and King (1982). This suggests that digestion and absorption of diets containing decreasing levels of protein were not different across protein levels. This also explains the lack of relationship between decreasing levels of protein and ADFI. This clearly highlights the need to redefine protein requirements for slow-growing indigenous pigs. Carter et al. (2016) reported that the nutrient requirements of local pigs are lower than those of exotic breeds. An increase in ADFI and ADG as the week proceeded could be associated with an increase in body size from about 24.4 kg in week 1 to 39.8 kg in week 8. As pigs grow, it is expected that feed intake would increase in view of increasing gut capacity.

A quadratic decrease in FCR as the dietary protein level decreased in the diet is in line with the findings of Barea et al. (2007) and Toledo et al. (2014). Similarly, Fang et al. (2019) reported that reducing dietary protein from 237 to 169 g/kg led response on the gain to feed ratio. The Windsnyer growing pig’s response to reduced dietary protein from 193 to 135.1 g/kg improved FCR (from 3.18 to 2.13) by 33%, but further reductions are not because FCR is compromised. The quadratic response in FCR to reduced dietary protein levels from 200 to 156 g/kg (Chrystal et al., 2020) and 197.5 to 162.5 g/kg (Greenhalgh et al., 2020) was reported in broiler. The negative effect of decremental levels of protein beyond the threshold to FCR could be associated with increased fat deposition. Literature indicates that monogastric animals offered low-protein diets have a high intake of energy than protein and the excess energy is deposited as lipid (Noblet et al., 2001; Chrystal et al., 2020; Greenhalgh et al., 2020). Low-protein diets in the present study were achieved through partial replacement of soybean meal with maize and wheat bran. This approach has been heavily linked to starch to protein disappearance rate ratio, which in turn affects the starch-protein digestive process (Chrystal et al., 2020; Greenhalgh et al., 2020). The response of Windsnyer pigs suggests that a rise in the amount of starch digested in proportion to that of protein compromises FCR.

A decrease in FCR as protein levels decrease emphasizes that protein needs for Windsnyer pigs are relatively lower than those of NRC (2012). An increase in FCR as protein levels further decrease beyond 135.1 g/kg suggests protein requirements must be kept around 135 g/kg. Further decrease in the protein level beyond 135.1 g/kg restricted muscle growth, resulting in the high amount of feed needed to increase a body mass by 1 kg. The level of dietary protein required for slow-growing Windsnyer pigs was lower than recommended by NRC (2012) for growing pigs in the body weight range of 25–50 kg.

Reducing dietary protein level from 193 to 135.1 g/kg improved the body weight (from 39.5 to 45.1 kg) and metabolic body weight (from 12.9 to 14.6) by 14.2%, and further reduction led to compromised body weight (34.7 kg) and metabolic body weight (11.4). A quadratic increase in FBW and BW0.75 as dietary protein level decreased could be associated with crude protein to energy ratio and non-essential amino acids in the diet. The reported results are concordant with findings by Liu et al. (2015) who found that low dietary protein/energy ratio did not affect the growth performance of Bama mini-pigs, a Chinese indigenous mini-pig breed. An increase in body weight with decreasing levels of protein could be associated with the high availability of dietary energy for fat deposition (Wang et al., 2018). Thus, carcass traits of pork fed decreasing levels of proteins warrant investigation. The growth and development process of pigs such as a change in the body weight depends directly on the breed and diet (Liu et al., 2015). It remains unclear how the dietary nutrients facilitate the effect of genetic difference on growth performance. The poor performance of Windsnyer pigs fed diet containing less than 135.1 g/kg could be associated with starch to protein ratio and non-essential amino acids. Further research is needed to understand the reason slow-growing Windsnyer pigs fed apparently required levels of amino acids but with less than 135.1 g/kg crude protein compromised performance.

Using the broken stick model, reduction of protein levels in the diet beyond 135.1 g/kg constrained FCR. To maximize FCR while meeting protein requirements for growing Windsnyer pigs, these pigs need to be supplied with 70% of 193 g/kg (a protein standard recommendation by the National Research Council 2012). Reduction of dietary protein levels beyond 135.1 g/kg compromised FBW and BW0.75 indigenous Windsnyer pigs. To optimize body weight in growing Windsnyer pigs while meeting protein requirements, protein levels need to be around 135.1 g/kg. Hlatini et al. (2020) reported that reducing dietary protein level from 193 to 135.1 g/kg led to improved nitrogen excretion, nitrogen utilization and biological value of feed protein in growing Windsnyer pigs. Reduction in dietary protein levels in the diets of indigenous Windsnyer pigs is necessary from an economic and environmental point of view. To consolidate investigation on protein requirements for growing Windsnyer pigs, there is a need for further research on digestibility, blood metabolites and carcass and pork quality. The availability of Windsnyer pigs for the experiment limited the study from estimating equation for protein to predict performance. Therefore, instead of more, only five pigs in each experimental diets were used.

Conclusions

Feed conversion ratio decreased with decreasing levels of protein. Body weight increased with decreasing levels of protein. Growing Windsnyer pigs require protein levels of 135.1 g/kg in the diets for maximum FCR, BW0.75 and FBW. Gradual reduction of dietary protein level did not compromise the growth performance of growing Windsnyer pigs until protein levels reached 135.1 g/kg. The present information on protein requirement on Windsnyer indigenous pigs can be useful to pig feed compounders and pig breeders and farmers.