| Literature DB >> 33479891 |
Vanessa Yenson1,2, Nicole Baumgarth3,4.
Abstract
B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5+ and CD5- B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5- B-1 cells by flow cytometry.Entities:
Keywords: B-1 cell isolation; B-cell isolation; B-cell subsets; Innate-like B cells; Natural antibodies; Peritoneal cavity
Mesh：
Substances：
Year: 2021 PMID： 33479891 DOI： 10.1007/978-1-0716-1237-8_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745