Zhiqiang Wu1,2, Yelin Han3, Bo Liu3, Hongying Li4, Guangjian Zhu4, Alice Latinne4,5,6, Jie Dong3, Lilin Sun3, Haoxiang Su3, Liguo Liu3, Jiang Du3, Siyu Zhou3, Mingxing Chen3, Anamika Kritiyakan7, Sathaporn Jittapalapong7, Kittipong Chaisiri8, Phillipe Buchy9, Veasna Duong10, Jian Yang3, Jinyong Jiang11, Xiang Xu11, Hongning Zhou11, Fan Yang3, David M Irwin12, Serge Morand7, Peter Daszak4, Jianwei Wang3, Qi Jin13. 1. NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. wuzq2009@ipbcams.ac.cn. 2. Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. wuzq2009@ipbcams.ac.cn. 3. NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. 4. EcoHealth Alliance, New York, NY, USA. 5. Wildlife Conservation Society, Viet Nam Country Program, Ha Noi, Vietnam. 6. Wildlife Conservation Society, Health Program, Bronx, NY, USA. 7. Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand. 8. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. 9. GlaxoSmithKline Vaccines, Singapore City, Singapore. 10. Virology Unit, Institut Pasteur in Cambodia, Phnom Penh, Cambodia. 11. Yunnan Institute of Parasitic Diseases, Pu'er, PR China. 12. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada. 13. NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China. zdsys@vip.sina.com.
Abstract
BACKGROUND: As the largest group of mammalian species, which are also widely distributed all over the world, rodents are the natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding of the core virome of diverse rodents should therefore assist in efforts to reduce the risk of future emergence or re-emergence of rodent-borne zoonotic pathogens. RESULTS: This study aimed to describe the viral range that could be detected in the lungs of rodents from Mainland Southeast Asia. Lung samples were collected from 3284 rodents and insectivores of the orders Rodentia, Scandentia, and Eulipotyphla in eighteen provinces of Thailand, Lao PDR, and Cambodia throughout 2006-2018. Meta-transcriptomic analysis was used to outline the unique spectral characteristics of the mammalian viruses within these lungs and the ecological and genetic imprints of the novel viruses. Many mammalian- or arthropod-related viruses from distinct evolutionary lineages were reported for the first time in these species, and viruses related to known pathogens were characterized for their genomic and evolutionary characteristics, host species, and locations. CONCLUSIONS: These results expand our understanding of the core viromes of rodents and insectivores from Mainland Southeast Asia and suggest that a high diversity of viruses remains to be found in rodent species of this area. These findings, combined with our previous virome data from China, increase our knowledge of the viral community in wildlife and arthropod vectors in emerging disease hotspots of East and Southeast Asia. Video abstract.
BACKGROUND: As the largest group of mammalian species, which are also widely distributed all over the world, rodents are the natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding of the core virome of diverse rodents should therefore assist in efforts to reduce the risk of future emergence or re-emergence of rodent-borne zoonotic pathogens. RESULTS: This study aimed to describe the viral range that could be detected in the lungs of rodents from Mainland Southeast Asia. Lung samples were collected from 3284 rodents and insectivores of the orders Rodentia, Scandentia, and Eulipotyphla in eighteen provinces of Thailand, Lao PDR, and Cambodia throughout 2006-2018. Meta-transcriptomic analysis was used to outline the unique spectral characteristics of the mammalian viruses within these lungs and the ecological and genetic imprints of the novel viruses. Many mammalian- or arthropod-related viruses from distinct evolutionary lineages were reported for the first time in these species, and viruses related to known pathogens were characterized for their genomic and evolutionary characteristics, host species, and locations. CONCLUSIONS: These results expand our understanding of the core viromes of rodents and insectivores from Mainland Southeast Asia and suggest that a high diversity of viruses remains to be found in rodent species of this area. These findings, combined with our previous virome data from China, increase our knowledge of the viral community in wildlife and arthropod vectors in emerging disease hotspots of East and Southeast Asia. Video abstract.
Authors: Patrick C Y Woo; Susanna K P Lau; Beatrice H L Wong; Annette Y P Wong; Rosana W S Poon; Kwok-Yung Yuen Journal: J Virol Date: 2011-12 Impact factor: 5.103
Authors: Jana Sachsenröder; Anne Braun; Patrycja Machnowska; Terry Fei Fan Ng; Xutao Deng; Sebastian Guenther; Samuel Bernstein; Rainer G Ulrich; Eric Delwart; Reimar Johne Journal: J Gen Virol Date: 2014-08-13 Impact factor: 3.891
Authors: Kate E Jones; Nikkita G Patel; Marc A Levy; Adam Storeygard; Deborah Balk; John L Gittleman; Peter Daszak Journal: Nature Date: 2008-02-21 Impact factor: 49.962
Authors: Siddharth Sridhar; Cyril C Y Yip; Shusheng Wu; Jianpiao Cai; Anna Jin-Xia Zhang; Kit-Hang Leung; Tom W H Chung; Jasper F W Chan; Wan-Mui Chan; Jade L L Teng; Rex K H Au-Yeung; Vincent C C Cheng; Honglin Chen; Susanna K P Lau; Patrick C Y Woo; Ning-Shao Xia; Chung-Mau Lo; Kwok-Yung Yuen Journal: Emerg Infect Dis Date: 2018-12 Impact factor: 6.883