| Literature DB >> 33476017 |
Kowsalya Devi Pavuluri1, Lorena Consolino2, Dario Livio Longo3, Pietro Irrera4, Phillip Zhe Sun5,6,7, Michael T McMahon8,9.
Abstract
Chemical exchange saturation transfer (CEST) is recognized as one of the premier methods for measuring pH with this environmental variable expected to be an excellent biomarker for kidney diseases. Here we describe step-by-step CEST MRI experimental protocols for producing pH and perfusion maps for monitoring kidney pH homeostasis in rodents after administering iopamidol as contrast agent. Several CEST techniques, acquisition protocols and ratiometric approaches are described. The impact of length of acquisition time on the quality of the maps is detailed. These methods may be useful for investigating progression in kidney disease in vivo for rodent models.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol is complemented by two separate chapters describing the basic concepts and data analysis.Entities:
Keywords: Chemical exchange saturation transfer (CEST); Contrast agent; Iopamidol; Kidney; Magnetic resonance imaging (MRI); Mice; Rats; Responsive contrast agent; pH imaging
Year: 2021 PMID： 33476017 DOI： 10.1007/978-1-0716-0978-1_27
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745