Jie Liu1,2, Fuwei Li1,3, Xin Hu4,5, Dingguo Cao1,2, Wei Liu1, Haixia Han1, Yan Zhou6,7, Qiuxia Lei8,9. 1. Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China. 2. Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, 250023, China. 3. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Ji'nan, 250023, China. 4. Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Beijing, 100193, China. 5. Molecular and Cellular Biology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, 5030, Belgium. 6. Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China. sally7919@163.com. 7. Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Ji'nan, 250023, China. sally7919@163.com. 8. Shandong Academy of Agricultural Sciences, Poultry Institute, Ji'nan, 250023, China. lei_qiuxia@163.com. 9. Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, 250023, China. lei_qiuxia@163.com.
Abstract
BACKGROUND: miRNAs play critical roles in growth and development. Various studies of chicken muscle development have focused on identifying miRNAs that are important for embryo or adult muscle development. However, little is known about the role of miRNAs in the whole muscle development process from embryonic to post-hatching periods. Here, we present a comprehensive investigation of miRNA transcriptomes at 12-day embryo (E12), E17, and day 1 (D1), D14, D56 and D98 post-hatching stages. RESULTS: We identified 337 differentially expressed miRNAs (DE-miRNAs) during muscle development. A Short Time-Series Expression Miner analysis identified two significantly different expression profiles. Profile 4 with downregulated pattern contained 106 DE-miRNAs, while profile 21 with upregulated pattern contained 44 DE-miRNAs. The DE-miRNAs with the upregulated pattern mainly played regulatory roles in cellular turnover, such as pyrimidine metabolism, DNA replication, and cell cycle, whereas DE-miRNAs with the downregulated pattern directly or indirectly contributed to protein turnover metabolism such as glycolysis/gluconeogenesis, pyruvate metabolism and biosynthesis of amino acids. CONCLUSIONS: The main functional miRNAs during chicken muscle development differ between embryonic and post-hatching stages. miRNAs with an upregulated pattern were mainly involved in cellular turnover, while miRNAs with a downregulated pattern mainly played a regulatory role in protein turnover metabolism. These findings enrich information about the regulatory mechanisms involved in muscle development at the miRNA expression level, and provide several candidates for future studies concerning miRNA-target function in regulation of chicken muscle development.
BACKGROUND: miRNAs play critical roles in growth and development. Various studies of chicken muscle development have focused on identifying miRNAs that are important for embryo or adult muscle development. However, little is known about the role of miRNAs in the whole muscle development process from embryonic to post-hatching periods. Here, we present a comprehensive investigation of miRNA transcriptomes at 12-day embryo (E12), E17, and day 1 (D1), D14, D56 and D98 post-hatching stages. RESULTS: We identified 337 differentially expressed miRNAs (DE-miRNAs) during muscle development. A Short Time-Series Expression Miner analysis identified two significantly different expression profiles. Profile 4 with downregulated pattern contained 106 DE-miRNAs, while profile 21 with upregulated pattern contained 44 DE-miRNAs. The DE-miRNAs with the upregulated pattern mainly played regulatory roles in cellular turnover, such as pyrimidine metabolism, DNA replication, and cell cycle, whereas DE-miRNAs with the downregulated pattern directly or indirectly contributed to protein turnover metabolism such as glycolysis/gluconeogenesis, pyruvate metabolism and biosynthesis of amino acids. CONCLUSIONS: The main functional miRNAs during chicken muscle development differ between embryonic and post-hatching stages. miRNAs with an upregulated pattern were mainly involved in cellular turnover, while miRNAs with a downregulated pattern mainly played a regulatory role in protein turnover metabolism. These findings enrich information about the regulatory mechanisms involved in muscle development at the miRNA expression level, and provide several candidates for future studies concerning miRNA-target function in regulation of chicken muscle development.
Entities:
Keywords:
Breast muscle; Differential expression profiles; Muscle development; miRNA transcriptome
Authors: Susan M Krzysik-Walker; Jill A Hadley; Jane E Pesall; Douglas C McFarland; Regina Vasilatos-Younken; Ramesh Ramachandran Journal: Comp Biochem Physiol A Mol Integr Physiol Date: 2011-04-22 Impact factor: 2.320
Authors: Ruoxi Hu; Wenchi Pan; Alexey V Fedulov; William Jester; Matthew R Jones; Scott T Weiss; Reynold A Panettieri; Kelan Tantisira; Quan Lu Journal: FASEB J Date: 2014-02-12 Impact factor: 5.191
Authors: Leonardo N Moraes; Geysson J Fernandez; Ivan J Vechetti-Júnior; Paula P Freire; Rodrigo W A Souza; Rolando A R Villacis; Silvia R Rogatto; Patricia P Reis; Maeli Dal-Pai-Silva; Robson F Carvalho Journal: Sci Rep Date: 2017-08-01 Impact factor: 4.379