The effect of age On cervical cancer screening in women aged 20-29.

No definite consensus exists currently regarding the appropriate age at which to start cervical cancer screening. We analyzed the effectiveness of age in abnormal histology outcomes in women aged 20-29. Data on women aged 20-29 having undergone opportunistic cervical cancer screening with cytology during the 2014-2019 period were retrospectively reviewed. Based on cytology outcomes, human papillomavirus test results (if present), age and clinical decision, patients underwent either colposcopy or observation. The effects of age and other epidemiologic factors on histologic diagnoses of cervical intraepithelial neoplasia (CIN) or cancer [CIN (+)] were analyzed in univariate and binomial logistic regression analyses. Among 1649 women, CIN (+) lesions were observed in 61 (3.7%) women. The occurrence of CIN (+) lesions increased 1.149 times each year; thus, women aged 25-29 were more likely to have CIN (+) than those aged 20-24 (4.4% vs. 2.1%; p=0.019). A significant determinant of CIN (+) was the increase in age, i.e. women aged 20-29. Accordingly, considering age is crucial for the diagnosis of CIN (+) in cancer screening.

Introduction

Cervical cancer is the fourth most commonly diagnosed and the fourth leading cause of death in females (). It is estimated that almost 570,000 new cases of cervical cancer and 311,000 associated deaths among women occur worldwide each year (). In Turkey, cervical cancer is the 9th most common cancer, with an estimated incidence rate of 4.3/100,000 (). It is understood that regular cervical cancer screening promotes early detection of cervical premalignant lesions and decreases mortality rate from cervical cancer. However, there is no consensus regarding the starting age for cervical cancer screening worldwide. Starting screening at 20 years of age may have little effect on detecting cervical cancer but may carry potential harm due to the treatment of high-grade cervical lesions that may regress with time, leading to adverse pregnancy outcomes such as preterm birth and low birth weight (). On the other hand, starting cervical cancer screening at the age of 30 years may lead to missing cervical intraepithelial neoplasia (CIN) lesions that have the potential risk of developing into cervical cancer.

The age for starting cervical cancer screening is between 20-30 in most countries globally. Turkey boasts a national cervical cancer screening program involving human papillomavirus (HPV) testing starting at the age of 30. For younger ages, opportunistic screening is available. Cervical cancer screening is individual-based and performed depending on the decisions of the clinicians and patients involved. There are limited data on the prevalence rates of CIN and cervical cancer in women aged 20-29 in Turkey. We therefore sought to analyze the prevalence of abnormal screening results among women aged 20-29 years in the Akdeniz University Department of Obstetrics and Gynecology to elucidate the prevalence of abnormal cytologic results not screened for in the national program. We also aimed to gather scientific evidence for the implementation of cervical cancer screening among women aged 20-29 by analyzing the effects of age and possible epidemiologic cofactors on developing colposcopy diagnosed abnormal histology outcomes.

Patients and Methods

After approval of the study was granted by the local institutional Ethics Committee (approval no. 818), we retrospectively investigated women aged 20-29 (<30) having undergone cervical cancer screening with cytology between 2014 and 2019. The study was performed in accordance with the ethical standards described in the appropriate version of the 1975 Declaration of Helsinki, as revised in 2000.

Patients with a known history of CIN or gynecologic cancer, and those that were pregnant were excluded from this study. If a woman underwent two or more cytologic investigations during the study period, the age at which abnormal cytology results were observed was taken into account. Conversely, if all cytology findings were normal, then the mean age among all the times the patient was examined was calculated and taken into account on data analysis. Only patients with satisfactory cytology outcomes were included in the analysis. If a patient had normal cytology but an absent/insufficient endocervical/transformation zone, she was excluded from this study.

Demographic data, marital status, past obstetric and gynecologic history, gravidity, parity, number of abortions, current use of a contraceptive method, smoking habit, cytology, HPV test results (if available), and cervical biopsy results were collected. Considering the need for colposcopy according to the American Society for Colposcopy and Cervical Pathology (ASCCP) guidelines, women who had not undergone colposcopy were excluded from statistical analysis performed for the association of age and other characteristics with CIN lesions.

Liquid-based cytology was used in all women. Normal cytology was defined as negative for intraepithelial lesion or malignancy. Cytology results were reported according to the Bethesda classification, as follows: abnormal findings included atypical squamous cells of undetermined significance (ASC-US); atypical squamous cells suspicious for high-grade squamous intraepithelial lesions (ASC-H); low-grade squamous intraepithelial lesions (LSIL); high-grade squamous intraepithelial lesions (HSIL); atypical glandular cells (AGC); adenocarcinoma in situ; and invasive cancer.

The management of abnormal cytology results was performed according to the ASCCP guidelines. Based on cytology outcomes, HPV test results (if present), patient age and clinical decision, patients underwent either colposcopy or observation with repeat screening testing. According to this management protocol, women aged 21-24 with ASC-US or L-SIL cytology were those who underwent colposcopy (e.g., if a patient had postcoital bleeding, obvious cervical lesions, and condyloma accuminata, she underwent colposcopy); otherwise, the patient underwent repeat cytology at 12 months later. At 12 months, if the patient had ASC-H, AGC, or HSIL, colposcopy was performed, but if the findings were negative, ASC-US or LSIL without any clinical suspicion, then the patient underwent repeat cytology again 12 months later, and so forth. If the patient had ASC-US with negative HPV test results, she was allocated to the CIN (-) group. The women aged 25 or older with ASC-US cytology without any clinical suspicion underwent HPV testing (if available during the study period); otherwise, repeat cytology was planned at one year and colposcopy was performed according to cytology results at one year. The women aged 25 or older with LSIL cytology without any clinical suspicion underwent colposcopy alone or colposcopy in conjunction either with HPV testing or follow-up cytology at one year. Women of all ages with ASC-H or HSIL findings underwent colposcopy. Standard colposcopic techniques were used on colposcopy, including observing the cervix after the application of 3% acetic acid and taking biopsies from all abnormal areas.

The prevalence of cytology findings was analyzed in the population of women in whom cytology was performed. Colposcopic biopsies were reported as normal (no CIN lesions), CIN grade 1, CIN grade 2, CIN grade 3, and invasive cancer. CIN grade 1 lesions were also defined as histology-proven LSIL, while CIN grades 2 and 3 lesions were defined as histology-proven HSIL.

Statistical method

Statistical Package for the Social Sciences version 23.0 for Windows software program (IBM Corp., Armonk, NY, USA) was used for statistical analyses. Frequencies of normal and abnormal cytology findings and HPV were calculated using descriptive statistics in the whole study population. The occurrence of histologically diagnosed CIN lesions or cancer was defined as the response variable [CIN (+)]. Continuous variables included age, gravidity, and parity. These parameters were evaluated by visual (e.g., histograms and probability plots) and analytical (Kolmogorov-Smirnov/Shapiro-Wilk test) methods to assess whether they were normally distributed or not. Abnormally distributed parameters were expressed as medians and percentiles, whereas normally distributed parameters were expressed as means. Categorical variables (e.g., any contraceptive method, smoking habit, age range 21-24 or 25-29) were expressed as numbers and frequencies. The χ2-test was used to evaluate the relationship of those categorical variables with the occurrence of CIN (+). Women with abnormal cytology who were neither followed-up with nor received colposcopy in the Akdeniz University Department of Obstetrics and Gynecology were not included in statistical analysis of CIN (+) occurrence.

A p-value of 0.25 on univariate analysis was considered as a restrictive alpha level. Therefore, parameters with p-values <0.25 on univariate analysis were included in the binomial logistic regression analysis. The forward likelihood ratio method was used and p-values <0.05 were defined as statistically significant in binomial logistic regression analysis.

Results

A total of 1756 women screened by cytology were evaluated in this study. Thirteen women with CIN diagnosis, nine women whose epidemiological characteristics could not be determined, three women with ovarian cancer diagnosis, two women with cervical cancer diagnosis, and one woman having undergone hysterectomy for benign reasons were excluded from the study. Thus, 1728 women with smear results were evaluated.

On cytologic examinations of 1728 women, 1521 (88.0%) women were found to be normal, 130 (7.5%) women were ASCUS, 55 (3.2%) women were LSIL, 14 (0.8%) women were ASCH, five (0.3%) women were HSIL, and three (0.2%) women were AGC. HPV was tested in 382 women and 95 (24.9%) of these were HPV (+).

Seventy-nine women with abnormal cytology but who were neither followed-up with nor received colposcopy in the Akdeniz University Department of Obstetrics and Gynecology could not be evaluated on univariate analysis or subsequent regression analysis. Thus, the effects of age and epidemiological factors on CIN (+) was investigated in 1649 women aged 20-29. CIN (+) lesions were observed in 61 (3.7%) women. Table 1 shows the epidemiological and clinical parameters of these 1649 women. Their median age was 26 years, with 1126 women being aged 25-29. Most of the women (94.6%) were not using any medically prescribed contraceptive method.

Table 1

Epidemiological and clinical parameters of study population

Characteristic	Parameter	Value (%)
Age (years)	Median25th-75th percentiles20-2425-29	2624-28523 (31.7)1126 (68.3)
Parity	Median25th-75th percentiles	00-1
Marital status	SingleMarried	596 (36.1)1053 (63.9)
Smoking habit	SmokerNon-smoker	197 (11.9)1452 (88.1)
Contraception method	NoOral contraceptive pillsIntrauterine deviceCondom	1560 (94.6)43 (2.6)36 (2.2)10 (0.6)

Epidemiological and clinical parameters in patients with cytologically or colposcopically normal or histologically diagnosed CIN (+) lesions are presented in Table 2. In comparison with women aged 20-24, those aged 25-29 were more likely to have CIN (+) (4.4% vs. 2.1%; p=0.019). The parameters of age, parity, smoking habit, use of oral contraceptive pills, and use of intrauterine device were included in the binomial logistic regression analysis. The only significant determinant of CIN (+) was the increase in age, thus the occurrence of CIN (+) lesions increased 1.149 times each year in women aged 20-29 (Table 3).

Table 2

Epidemiological and clinical parameters in patients with cytologically or colposcopically normal or histologically diagnosed CIN (+) lesions

Characteristic	Parameter	Cytology/colposcopy finding/biopsy (%)		Univariate analysis
		Normal	CIN (+)	p value
Age (years)	MedianIQR20-2425-29	264512 (97.9)1076 (95.6)	27311 (2.1)50 (4.4)	0.019b0.019a
Parity	MedianIQR	01	01	0.012
Smoking habit	SmokerNon-smoker	185 (93.9)1403 (96.6)	12 (6.1)49 (3.4)	0.058a
Marital status	SingleMarried	570 (95.6)1018 (96.7)	26 (4.4)35 (3.3)	0.283a
Use of oral contraceptive pills	YesNo	40 (93.0)1548 (96.4)	3 (7.0)58 (3.6)	0.211c
Use of intrauterine device	YesNo	33 (91.7)1555 (96.4)	3 (8.3)58 (3.6)	0.145c

CIN (+) = cervical intraepithelial neoplasia or cancer; IQR = interquartile range; aMann Whitney U test; bPearson’s χ2-test; cFisher exact test

Table 3

Logistic regression analysis of factors predictive of CIN (+)

	Coefficient	SE	Wald	df	p	OR	95% CI for OR
							Lower	Upper
Age (years)	0.139	0.056	6.078	1	0.014	1.149	1.029	1.284

SE = standard error; df = degree of freedom; OR = odds ratio; CI = confidence interval; CIN (+) = cervical intraepithelial neoplasia grade 1 or higher; Hosmer and Lemeshow test value: 0.673

Discussion

This study analyzed cytology and histology outcomes in the context of examining the effects of age and epidemiologic cofactors on CIN (+) in women aged 20-29. According to our results, while 12% of women had abnormal cytology results, HPV testing was positive in 24.9% of women aged 20-29. We also analyzed the effect of age on the occurrence of CIN (+) lesions in women aged 20-29 and found a one-year increase in age in these women to increase the occurrence of CIN (+) lesions 1.149 times.

Our study population was from the region of Turkey with one of the highest HPV rates (). In the 30-65 age group, the HPV (+) rate is 4.4% nationally in Turkey but 6.2% in Antalya (). In our study group, 24.9% of women aged 20-29 were HPV (+).

The reason for the high HPV (+) rate in our study as compared with data from the national HPV screening initiatives is the fact that the prevalence of HPV in our region is highest in Turkey and the study population was of a younger age. Indeed, persistent HPV infection is mandatory for the development of CIN and cervical cancer (). HPV acquisition in a young female is believed to occur just after her becoming sexually active, although the majority of this infection or early lesions become undetectable within one to two years (, ). High-risk HPV infection is relatively more common in females aged 20-29, with the highest incidence rate of 43.4% observed among those aged 20-24 (). However, the incidence rate of cervical cancer is one of the lowest in this age group. This is because of the transient nature of HPV infection, and also because cervical cancer takes about 10 to 15 years after the onset of HPV infection to develop. Thus, women with the disease diagnosed at 30-40 years of age may have a history of CIN in their twenties. In parallel to HPV prevalence, 12% of our study group had abnormal cytology results. In a comprehensive study by the Turkish Cervical Cancer and Cervical Cytology Research Group, the abnormal cytology prevalence rate in women of all ages was 1.8% (). Additionally, a 9% to 19% prevalence rate of abnormal cytology in adolescents and young adults has been reported in previous studies (, ). These higher rates of cytologic abnormalities as compared with adult population can be explained by the increased HPV infection rate at these ages.

Previous studies have shown that age plays an important role in the development of CIN and cervical cancer (-). In our study, we found that women aged 25-29 in comparison with women aged 20-24 were more likely to have CIN (+) (4.4% vs. 2.1%; p=0.019). Logistic regression analysis revealed that the only determinant of CIN (+) was an increase in age. This means that the risk increase of CIN observed each year was valid for all ages in comparison with previous age. For example, women aged 27 were 1.149 times more likely to have CIN in comparison with women aged 26. Untreated CIN regresses, persists or progresses, and the rate of progression is higher in older women with CIN grades 2 and 3 (). CIN lesions require close observation or treatment. Treatment is completed via excisional procedures when CIN grades 2 or 3 are diagnosed or when persistence of CIN grade 1 is observed. However, excisional procedures are related to adverse pregnancy outcomes; therefore, observation is another option for women planning to be pregnant in the future. With this strategy, unnecessary treatment is not applied to the lesions to force regression and the side effects of treatment are prevented. In an observational cohort study from the United States (US), the annual incidence of abnormal cytology and CIN in women of all ages who undergo routine cervical cancer screening is reported to be about 5% and 2.7%, respectively (). The same research revealed that the most common age group demonstrating abnormal cytology results and CIN were those aged 20-29 (). CIN grade 1 was most commonly diagnosed in women aged 20-24 (5.1/1000), while CIN grades 2 and 3 were most commonly diagnosed in women aged 25-29 (4.1/1000) (). Similarly, Butorac et al. report that CIN was most commonly diagnosed in younger women and therefore surgical treatment is more commonly applied to women aged 25-35 in Croatia (). The rate of CIN in our study (36.9/1000) was high as compared with the previous research in women aged 20-29. We believe that our high rate of CIN recorded in this age group was due to the study population having the highest incidence rate of HPV in Turkey. The CIN incidence rates are decreasing in societies that include HPV vaccine on the vaccination schedule (). Thus, there should be new screening guidelines for cervical cancer implemented in those societies, including the screening starting age. In contrast, HPV vaccine is not included at this time in the national vaccination schedule in Turkey. Despite this, Turkey has already designated screening to begin at the age of 30. Opportunistic screening, which some younger women may undergo, has been shown to have no benefit in preventing cervical cancer (). Thus, organized screening must be implemented if cervical cancer screening is suggested to be done in women aged 20-29. A similar effect of age increase on the prevalence of cervical premalignant lesions has also been documented for the progression of untreated CIN lesions via screening. Older patients have higher rates of progression and lower rates of regression. Among women aged ≤24, 25-29, 30-34, 35-39 and ≥40, progression rate was 10.6%, 13.5%, 17.1%, 10.8% and 24.9%, respectively (). In the same study, it was shown that, independently of CIN grade and presence or absence of HPV lesions, the CIN lesion regression rate was reduced by 21% with each five-year age grouping ().

Guidelines around the world present different recommendations on the starting age for cervical cancer screening. The World Health Organization recommends that screening be initiated at the age of 30, while most European (e.g., France, Belgium, Ireland, Italy, Norway), Australian and Canadian guidelines recommend the age of 25 years as when to initiate screening (-). Separately, US guidelines recommend screening should start at 21 years of age (). Korean guidelines also recommend that screening should begin at 20 years due to the country’s increased occurrence of high-grade lesions and cervical carcinoma as compared with developed countries (). With these cut-off ages, many of these countries believe that the treatment of precancerous lesions during pregnancy will be prevented. In parallel to the screening guidelines of the countries, screening programs differ as well, and the starting age for screening is a controversial issue. Each country implements its cervical cancer screening policy depending upon its own cervical cancer statistics, internal dynamics, and HPV (+) rate. Moreover, HPV vaccine is included in the immunization schedule mostly depending on the economic power of the country.

Of note, Makkonen et al. showed that the performance of organized screening in Finland, which officially introduces screening at 30 years, had no protective effect on cervical cancer among women younger than 25; however, when performed at age 25-29, it decreased the risk of cervical cancer by 48% (). In contrast, Andrae et al. showed that Sweden’s national cervical cancer screening had a protective effect against cervical cancer among women aged 20-29. They pointed out that Sweden’s cervical cancer screening by cytology approach reduced the rate of cervical cancer cases by about 58% in women aged 20-29 ().

The aim of cervical cancer screening is to diagnose CIN before the onset of cancer. Thus, cervical cancer can be prevented by treatment of early lesions. The aim of studies analyzing the effectiveness of screening should not be focused on the detection rate of cancer. However, if we investigated the relationship between age and cervical cancer, we could see that the incidence of cervical cancer is related to the age of women. According to the International Agency for Research on Cancer, the estimated cervical cancer rates at ages 20, 25 and 30 years were about 0.30/100,000, 1.82/100,000 and 2.80/100,000, respectively. Also, the increase in the rate of cervical cancer between age 20 and 25 was slightly higher than the increase recorded between age 25 and 30 years (). In the United Kingdom (UK), cervical cancer incidence is highest in women aged 25-29 as compared with any other five-year period of a woman’s life. The incidence gradually decreases until age 60-64 and then rises again (). The cervical cancer incidence rates per 100,000 women-years were 4.0 at age 20-24 but 20.3 at age 25-29 (). In comparison, in the US, the cervical cancer incidence rate per 100,000 women-years was 1.40 at age 20-24 but 5.94 at age 25-29 ().

There are studies that address the roles of cofactors in the progression of cervical carcinoma in women of different age groups. In studies including adults older than 30, smoking, parity, and oral contraception and intrauterine device use were defined as cofactors for CIN and cervical cancer (-). Among adolescents and young adults, no correlation was found between smoking or use of oral contraceptives and CIN and HPV (). However, we found no relationship between these cofactors and the occurrence of CIN in women aged 20-29 either. Specifically, this may be due to the fact that temporary behavior of HPV in young women makes these cofactors ineffective in determining the progression of HPV to CIN and cervical cancer.

Study limitations

There were some limitations in our study that should be highlighted. Because it was a retrospective study, we were unable to question about the age of first sexual intercourse or the frequency and number of sexual partners of the women, which are the possible risk factors for HPV persistence in developing CIN and cervical cancer (). Another limitation was the lack of investigation of HPV subtypes, as a specific subtype may be more related to CIN development. It has been pointed out that the most carcinogenic subtypes (HPV subtypes 16 and 18) are most strongly related to high-grade precancerous lesions and cervical cancer in the 20-29 age group (). Moreover, HPV subtypes 16 and 18 cause CIN quicker and at younger ages, while other subtypes progress more slowly and cause CIN at older ages (). The final limitation of note was that the study population was from a single centre and the region (Antalya) displaying the upper limit of the HPV positivity in Turkey.

However, there also were significant advantages to this study. Methodologically, the women included in the study met the ASCCP guidelines. Also, the entire study population included women aged <30 who were not screened by the national cervical cancer screening initiative in Turkey. In this manner, the study offers valuable information about the results of smear, HPV, and colposcopy in this unscreened population.

Conclusion

According to our findings and those of previous studies, age plays an important role in the development of CIN (+). Thus, taking age in consideration is crucial in the diagnosis of CIN (+) on cancer screening. Women aged 25-29 as compared to those aged 20-24 are two times more likely to have CIN (+). CIN (+) lesions are 1.149 times more likely to develop with each one-year age progression. Although our study population did not represent the characteristics of the whole Turkey or the global population, our study population’s HPV positivity, abnormal cytology, and CIN rates were similar or higher than those of other populations that have undergone such screening. Therefore, starting screening at age 25 instead of age 30 should be considered in our region, and further extensive studies are needed to decide on the optimal age to implement cervical cancer screening.