Quanzhi Liang1, Ailijiang Asila1, Yingjie Deng1, Jun Liao1, Zhenfeng Liu2, Rui Fang3. 1. Department of Orthopedics, Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, No. 116, Huanghe Road, Shayibake District, 830000, Urumqi City, Xinjiang Province, People's Republic of China. 2. Department of Rehabilitation, the Ninth People's Hospital of Wuxi, 214000, Wuxi City, Jiangsu Province, People's Republic of China. 3. Department of Orthopedics, Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, No. 116, Huanghe Road, Shayibake District, 830000, Urumqi City, Xinjiang Province, People's Republic of China. ruifang1961@163.com.
Abstract
BACKGROUND: Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA. METHODS: Synovial fluid was extracted from both OA patients (n = 58) and healthy controls (n = 58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay. RESULTS: HOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation. CONCLUSIONS: Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
BACKGROUND: Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA. METHODS: Synovial fluid was extracted from both OA patients (n = 58) and healthy controls (n = 58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay. RESULTS: HOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation. CONCLUSIONS: Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
Authors: Ian J Wallace; Steven Worthington; David T Felson; Robert D Jurmain; Kimberly T Wren; Heli Maijanen; Robert J Woods; Daniel E Lieberman Journal: Proc Natl Acad Sci U S A Date: 2017-08-14 Impact factor: 11.205
Authors: Ali Mobasheri; Willem Evert van Spil; Emma Budd; Ilona Uzieliene; Eiva Bernotiene; Anne-Christine Bay-Jensen; Jonathan Larkin; Marc C Levesque; Oreste Gualillo; Yves Henrotin Journal: Curr Opin Rheumatol Date: 2019-01 Impact factor: 5.006