| Literature DB >> 33432151 |
Fernando Gordillo Altamirano1, John H Forsyth2, Ruzeen Patwa2, Xenia Kostoulias3, Michael Trim2, Dinesh Subedi2, Stuart K Archer4, Faye C Morris3, Cody Oliveira2, Luisa Kielty2, Denis Korneev2, Moira K O'Bryan2, Trevor J Lithgow3,5, Anton Y Peleg3,5,6, Jeremy J Barr7,8.
Abstract
We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective.Entities:
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Year: 2021 PMID： 33432151 DOI： 10.1038/s41564-020-00830-7
Source DB: PubMed Journal: Nat Microbiol ISSN： 2058-5276 Impact factor: 17.745