Carolina Bosso André1, Pedro Luiz Rosalen2, Marcelo Giannini3, Bruno Bueno-Silva4, Carmem Silvia Pfeifer5, Jack Liborio Ferracane6. 1. Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: carolina.bosso@gmail.com. 2. Department of Biosciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil; Biological Sciences Graduate Program, Federal University of Alfenas, Alfenas, MG, Brazil. Electronic address: pedro.rosalen@unifal-mg.edu.br. 3. Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil. Electronic address: gianinni@unicamp.br. 4. Dental Research Division, School of Dentistry, Guarulhos University, Guarulhos, SP, Brazil. Electronic address: brunobue@gmail.com. 5. Department of Biomaterials and Biomechanics, School of Dentistry, Oregon Health & Science University, Portland, OR, USA. Electronic address: pfeiferc@ohsu.edu. 6. Department of Biomaterials and Biomechanics, School of Dentistry, Oregon Health & Science University, Portland, OR, USA. Electronic address: ferracan@ohsu.edu.
Abstract
OBJECTIVES: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. METHODS: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. RESULTS: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. SIGNIFICANCE: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
OBJECTIVES: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. METHODS: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. RESULTS: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. SIGNIFICANCE: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
Authors: H Koo; M F Hayacibara; B D Schobel; J A Cury; P L Rosalen; Y K Park; A M Vacca-Smith; W H Bowen Journal: J Antimicrob Chemother Date: 2003-10-16 Impact factor: 5.790
Authors: L C C Galvão; P L Rosalen; I Rivera-Ramos; G C N Franco; J K Kajfasz; J Abranches; B Bueno-Silva; H Koo; J A Lemos Journal: Mol Oral Microbiol Date: 2016-05-16 Impact factor: 3.563
Authors: W Krzyściak; A Jurczak; D Kościelniak; B Bystrowska; A Skalniak Journal: Eur J Clin Microbiol Infect Dis Date: 2013-10-24 Impact factor: 3.267