Carolina Bosso André1, Pedro Luiz Rosalen2, Lívia Câmara de Carvalho Galvão3, Bruna Marin Fronza4, Glaucia Maria Bovi Ambrosano5, Jack Liborio Ferracane6, Marcelo Giannini4. 1. Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. Electronic address: carolina.bosso@gmail.com. 2. Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. 3. Department of Dentistry, Dom Bosco Graduate School and CEUMA University, São Luis, MA, Brazil. 4. Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. 5. Department of Social Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. 6. Department of Restorative Dentistry, School of Dentistry, Oregon Health & Science University, Portland, OR, USA.
Abstract
OBJECTIVES: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. METHODS: Apigenin (1mM) and tt-Farnesol (5mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. RESULTS: Addition of Apigenin and Apigenin+tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. SIGNIFICANCE: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.
OBJECTIVES: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. METHODS:Apigenin (1mM) and tt-Farnesol (5mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. RESULTS: Addition of Apigenin and Apigenin+tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. SIGNIFICANCE: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.
Authors: Aline Rogéria Freire de Castilho; Pedro Luiz Rosalen; Isaac Jordão de Souza Araújo; Igor Lebedenco Kitagawa; Cecilia Atem Gonçalves de Araújo Costa; Malvin N Janal; Marcelo Corrêa Alves; Simone Duarte; Paulo Noronha Lisboa Filho; Rafael Nobrega Stipp; Regina Maria Puppin-Rontani Journal: PLoS One Date: 2019-08-20 Impact factor: 3.240