Literature DB >> 33420996

Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma.

Viktoria Anselm1, Andreas Steinhilber1, Cornelia Sommersdorf1, Oliver Poetz2.   

Abstract

Targeted protein quantification can be challenging in body fluids such as plasma with regard to sensitivity and selectivity. In this chapter, we present a protocol for the quantification of high mobility group box 1 protein (HMGB1) in plasma using an immunoaffinity liquid chromatography mass spectrometric assay (IA-LC-MSMS). The protocol provides detailed assay instructions involving sample proteolysis, peptide-targeted immunoprecipitation, and LC-MSMS-based read out.

Entities:  

Keywords:  Antibody; HMGB1; Immunoaffinity liquid chromatography mass spectrometric assay; Immunoprecipitation; Parallel reaction monitoring; Peptide quantification

Mesh：

Substances：

Year:  2021        PMID： 33420996     DOI： 10.1007/978-1-0716-1186-9_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

Review 1.  A human proteome detection and quantitation project.

Authors:  N Leigh Anderson; Norman G Anderson; Terry W Pearson; Christoph H Borchers; Amanda G Paulovich; Scott D Patterson; Michael Gillette; Ruedi Aebersold; Steven A Carr
Journal:  Mol Cell Proteomics       Date:  2009-01-07       Impact factor: 5.911

2.  Evaluation of novel biomarkers of nephrotoxicity in Cynomolgus monkeys treated with gentamicin.

Authors:  Jean-Charles Gautier; Xiaobing Zhou; Yi Yang; Thierry Gury; Zhe Qu; Xavier Palazzi; Jean-François Léonard; Mohamed Slaoui; Yaligara Veeranagouda; Isabelle Guizon; Eric Boitier; Aziz Filali-Ansary; Bart H J van den Berg; Oliver Poetz; Thomas Joos; Tianyi Zhang; Jufeng Wang; Philippe Detilleux; Bo Li
Journal:  Toxicol Appl Pharmacol       Date:  2016-04-19       Impact factor: 4.219

3.  Reproducibility crisis: Blame it on the antibodies.

Authors:  Monya Baker
Journal:  Nature       Date:  2015-05-21       Impact factor: 49.962

4.  A proposal for validation of antibodies.

Authors:  Mathias Uhlen; Anita Bandrowski; Steven Carr; Aled Edwards; Jan Ellenberg; Emma Lundberg; David L Rimm; Henry Rodriguez; Tara Hiltke; Michael Snyder; Tadashi Yamamoto
Journal:  Nat Methods       Date:  2016-09-05       Impact factor: 28.547

Review 5.  Mass-spectrometric exploration of proteome structure and function.

Authors:  Ruedi Aebersold; Matthias Mann
Journal:  Nature       Date:  2016-09-15       Impact factor: 49.962

6.  Protein Biomarker Quantification by Immunoaffinity Liquid Chromatography-Tandem Mass Spectrometry: Current State and Future Vision.

Authors:  Hendrik Neubert; Christopher M Shuford; Timothy V Olah; Fabio Garofolo; Gary A Schultz; Barry R Jones; Lakshmi Amaravadi; Omar F Laterza; Keyang Xu; Bradley L Ackermann
Journal:  Clin Chem       Date:  2020-02-01       Impact factor: 8.327

7.  Plasma Proteome Profiling to Assess Human Health and Disease.

Authors:  Philipp E Geyer; Nils A Kulak; Garwin Pichler; Lesca M Holdt; Daniel Teupser; Matthias Mann
Journal:  Cell Syst       Date:  2016-03-23       Impact factor: 10.304

8.  Quantitative analysis of peptides and proteins in biomedicine by targeted mass spectrometry.

Authors:  Michael A Gillette; Steven A Carr
Journal:  Nat Methods       Date:  2013-01       Impact factor: 28.547

9.  Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations.

Authors:  David M Rissin; Cheuk W Kan; Todd G Campbell; Stuart C Howes; David R Fournier; Linan Song; Tomasz Piech; Purvish P Patel; Lei Chang; Andrew J Rivnak; Evan P Ferrell; Jeffrey D Randall; Gail K Provuncher; David R Walt; David C Duffy
Journal:  Nat Biotechnol       Date:  2010-05-23       Impact factor: 54.908

Review 10.  HMGB1: endogenous danger signaling.

Authors:  John R Klune; Rajeev Dhupar; Jon Cardinal; Timothy R Billiar; Allan Tsung
Journal:  Mol Med       Date:  2008 Jul-Aug       Impact factor: 6.354
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  1 in total

1.  Sample Preparation Strategies for Antibody-Free Quantitative Analysis of High Mobility Group Box 1 Protein.

Authors:  Ingeborg Kvivik; Grete Jonsson; Roald Omdal; Cato Brede
Journal:  Pharmaceuticals (Basel)       Date:  2021-06-03
  1 in total

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