Literature DB >> 33420983

Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry.

Ximo Zhang1.   

Abstract

In this chapter, we describe an LC-fluorescence (FLR)/MS-based method for released N-glycan analysis in the development of biotherapeutic proteins. The method includes enzymatic release and labeling of N-glycans with a signal-enhancing tag, LC-MS data collection, and data interpretation. Using the given protocol, up to 24 glycan samples can be prepared within 1 h, while the LC-FLR/MS data can be collected and analyzed using an established data processing method in a semi-automated manner.

Entities:  

Keywords:  Analytical testing; Biotherapeutic proteins; Glycoprofiling; LC-MS; N-glycan analysis; Released glycans sample preparation

Mesh：

Substances：

Year:  2021        PMID： 33420983     DOI： 10.1007/978-1-0716-1186-9_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  HILIC analysis of fluorescence-labeled N-glycans from recombinant biopharmaceuticals.

Authors:  Michael Melmer; Thomas Stangler; Mark Schiefermeier; Werner Brunner; Hansjörg Toll; Alfred Rupprechter; Wolfgang Lindner; Andreas Premstaller
Journal:  Anal Bioanal Chem       Date:  2010-07-17       Impact factor: 4.142

2.  Recent Liquid Chromatographic Approaches and Developments for the Separation and Purification of Carbohydrates.

Authors:  Gabe Nagy; Tianyuan Peng; Nicola L B Pohl
Journal:  Anal Methods       Date:  2017-05-24       Impact factor: 2.896

3.  Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Labeling Reagent that Facilitates Sensitive Fluorescence and ESI-MS Detection.

Authors:  Matthew A Lauber; Ying-Qing Yu; Darryl W Brousmiche; Zhengmao Hua; Stephan M Koza; Paula Magnelli; Ellen Guthrie; Christopher H Taron; Kenneth J Fountain
Journal:  Anal Chem       Date:  2015-05-08       Impact factor: 6.986

4.  Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity.

Authors:  Robert L Shields; Jadine Lai; Rodney Keck; Lori Y O'Connell; Kyu Hong; Y Gloria Meng; Stefanie H A Weikert; Leonard G Presta
Journal:  J Biol Chem       Date:  2002-05-01       Impact factor: 5.157

5.  Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry.

Authors:  Radoslaw P Kozak; Concepcion Badia Tortosa; Daryl L Fernandes; Daniel I R Spencer
Journal:  Anal Biochem       Date:  2015-06-12       Impact factor: 3.365

6.  Production, characterization, and pharmacokinetic properties of antibodies with N-linked mannose-5 glycans.

Authors:  Marcella Yu; Darren Brown; Chae Reed; Shan Chung; Jeff Lutman; Eric Stefanich; Anne Wong; Jean-Philippe Stephan; Robert Bayer
Journal:  MAbs       Date:  2012-07-01       Impact factor: 5.857

Review 7.  Glycan labeling strategies and their use in identification and quantification.

Authors:  L R Ruhaak; G Zauner; C Huhn; C Bruggink; A M Deelder; M Wuhrer
Journal:  Anal Bioanal Chem       Date:  2010-03-12       Impact factor: 4.142

Review 8.  The "less-is-more" in therapeutic antibodies: Afucosylated anti-cancer antibodies with enhanced antibody-dependent cellular cytotoxicity.

Authors:  Natasha A Pereira; Kah Fai Chan; Pao Chun Lin; Zhiwei Song
Journal:  MAbs       Date:  2018-07       Impact factor: 5.857

Review 9.  Glycan analysis of therapeutic glycoproteins.

Authors:  Lei Zhang; Shen Luo; Baolin Zhang
Journal:  MAbs       Date:  2015-11-24       Impact factor: 5.857

Review 10.  Fc glycans of therapeutic antibodies as critical quality attributes.

Authors:  Dietmar Reusch; Max L Tejada
Journal:  Glycobiology       Date:  2015-08-11       Impact factor: 4.313
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