| Literature DB >> 3342012 |
R S Feldberg1, D A Iannitti, D E Cochrane.
Abstract
A procedure for obtaining rat mast-cell histidine decarboxylase in greater than 50% yield in cell-free extracts was developed. The enzyme was found in the supernatant fractions from a 3,500 g and a 105,000 g centrifugation step and was demonstrated to be sensitive to inhibition by alpha-fluoromethylhistidine but not by phenylalanine. Although the enzyme shows a half-life of only 3 h in cell-free extract, the initial high recovery of activity allowed for active-site labelling of the enzyme by [3H]histidine and NaBH4. Labelled protein migrated on non-denaturing polyacrylamide-gradient-gel electrophoresis as a 55,000 Da species.Entities:
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Year: 1988 PMID： 3342012 PMCID： PMC1148697 DOI： 10.1042/bj2490297
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857