Jessy Azarcoya-Barrera1, Catherine J Field1, Susan Goruk1, Alexander Makarowski1, Jonathan M Curtis1, Yves Pouliot2, René L Jacobs1, Caroline Richard3. 1. Department of Agricultural, Food and Nutritional Science, Center for Health Research Innovation, University of Alberta, 4-002G Li Ka Shing, Edmonton, AB, T6G 2E1, Canada. 2. STELA Dairy Research Center, Institute of Nutrition and Functional Foods (INAF), Université Laval, Québec, Canada. 3. Department of Agricultural, Food and Nutritional Science, Center for Health Research Innovation, University of Alberta, 4-002G Li Ka Shing, Edmonton, AB, T6G 2E1, Canada. cr5@ualberta.ca.
Abstract
PURPOSE: To determine the effect of feeding buttermilk-derived choline metabolites on the immune system development in Sprague-Dawley rat pups. METHODS: Sprague-Dawley dams were randomized to one of the three diets containing 1.7 g/kg choline: 1-Control (100% free choline (FC)), 2-Buttermilk (BM, 37% phosphatidylcholine (PC), 34% sphingomyelin (SM), 17% glycerophosphocholine (GPC), 7% FC, 5% phosphocholine), and 3-Placebo (PB, 50% PC, 25% FC, 25% GPC) until the end of the lactation period. At weaning, pups continued on the same diet as their mom. Cell phenotypes and cytokine production by mitogen-stimulated splenocytes isolated from 3- and 10-week-old pups were measured. RESULTS: At 3 weeks, BM-pups had a higher proportion of cytotoxic T cells (CTL; CD3 + CD8 +) while both BM- and PB-pups had an increased proportion of cells expressing CD28 + , CD86 + and CD27 + (all p > 0.05). Following ConA stimulation, splenocytes from BM- and PB-pups produced more TNF-α and IFN-γ and after LPS stimulation produced more IL-10 and TNF-α (all p > 0.05). Starting at week 6 of age, BM-pups had a higher body weight. At 10 weeks, both the BM- and PB-pups had a higher proportion of CTL expressing CD27 + . After ConA stimulation, splenocytes from BM- and PB-pups produced more IL-2, IFN-γ and IL-6 and more IL-10 after LPS stimulation (all p > 0.05). CONCLUSION: The proportion of lipid soluble forms of choline in the diet during lactation and weaning periods influence the immune system development in rat offspring.
PURPOSE: To determine the effect of feeding buttermilk-derived choline metabolites on the immune system development in Sprague-Dawley rat pups. METHODS: Sprague-Dawley dams were randomized to one of the three diets containing 1.7 g/kg choline: 1-Control (100% free choline (FC)), 2-Buttermilk (BM, 37% phosphatidylcholine (PC), 34% sphingomyelin (SM), 17% glycerophosphocholine (GPC), 7% FC, 5% phosphocholine), and 3-Placebo (PB, 50% PC, 25% FC, 25% GPC) until the end of the lactation period. At weaning, pups continued on the same diet as their mom. Cell phenotypes and cytokine production by mitogen-stimulated splenocytes isolated from 3- and 10-week-old pups were measured. RESULTS: At 3 weeks, BM-pups had a higher proportion of cytotoxic T cells (CTL; CD3 + CD8 +) while both BM- and PB-pups had an increased proportion of cells expressing CD28 + , CD86 + and CD27 + (all p > 0.05). Following ConA stimulation, splenocytes from BM- and PB-pups produced more TNF-α and IFN-γ and after LPS stimulation produced more IL-10 and TNF-α (all p > 0.05). Starting at week 6 of age, BM-pups had a higher body weight. At 10 weeks, both the BM- and PB-pups had a higher proportion of CTL expressing CD27 + . After ConA stimulation, splenocytes from BM- and PB-pups produced more IL-2, IFN-γ and IL-6 and more IL-10 after LPS stimulation (all p > 0.05). CONCLUSION: The proportion of lipid soluble forms of choline in the diet during lactation and weaning periods influence the immune system development in rat offspring.
Authors: Neele S Dellschaft; Megan R Ruth; Susan Goruk; Erin D Lewis; Caroline Richard; René L Jacobs; Jonathan M Curtis; Catherine J Field Journal: Br J Nutr Date: 2015-04-23 Impact factor: 3.718
Authors: Erin D Lewis; Caroline Richard; Susan Goruk; Emily Wadge; Jonathan M Curtis; René L Jacobs; Catherine J Field Journal: Nutrients Date: 2017-07-07 Impact factor: 5.717
Authors: Yongbo She; Kun Wang; Alexander Makarowski; Rabban Mangat; Sue Tsai; Benjamin P Willing; Spencer D Proctor; Caroline Richard Journal: Eur J Nutr Date: 2022-10-05 Impact factor: 4.865
Authors: Jessy Azarcoya-Barrera; Bethany Wollin; Hellen Veida-Silva; Alexander Makarowski; Susan Goruk; Catherine J Field; René L Jacobs; Caroline Richard Journal: Front Nutr Date: 2022-02-02
Authors: Yongbo She; Kun Wang; Alexander Makarowski; Rabban Mangat; Sue Tsai; Benjamin P Willing; Spencer D Proctor; Caroline Richard Journal: Front Nutr Date: 2022-06-17