Qiming Lv1,2, Xiuli Zhang1,2, Dingyang Yuan1,2, Zhiyuan Huang1, Rui Peng3, Jiming Peng1,2, Zuren Li4, Li Tang1,2, Ducai Liu5, Xiaomao Zhou4, Lifeng Wang4, Lang Pan4, Ye Shao1, Bigang Mao1,2, Yeyun Xin1,2, Lihuang Zhu6,7, Bingran Zhao8,9, Lianyang Bai10,11,12. 1. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, China. 2. Longping Branch of Graduate School, Hunan University, Changsha, China. 3. Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China. 4. Hunan Agricultural Biotechnology Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China. 5. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China. 6. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, China. lhzhu@genetics.ac.cn. 7. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. lhzhu@genetics.ac.cn. 8. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, China. brzhao652@hhrrc.ac.cn. 9. Longping Branch of Graduate School, Hunan University, Changsha, China. brzhao652@hhrrc.ac.cn. 10. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, China. lybai@hunaas.cn. 11. Longping Branch of Graduate School, Hunan University, Changsha, China. lybai@hunaas.cn. 12. Hunan Agricultural Biotechnology Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China. lybai@hunaas.cn.
Abstract
BACKGROUND: Benzobicyclon (BBC) is a β-triketone herbicide (bTH) used in rice paddy fields. It has the advantages of high efficiency, low toxicity, high crop safety, and good environmental compatibility, and shows efficacy against paddy weeds resistant to other types of herbicides. However, as some important indica rice varieties are susceptible to BBC, BBC is currently only registered and applied in japonica rice cultivation areas. RESULTS: By analyzing haplotypes of the bTHs broad-spectrum resistance gene HIS1 and phenotypes for BBC in 493 major indica rice accessions in China, we identified a novel non-functional allelic variant of HIS1 in addition to the previously reported 28-bp deletion. Through detection with markers specific to the two non-functional mutations, it was clear that 25.4% of indica conventional varieties, 59.9% of fertility restorers, and 15.9% of sterile lines were susceptible to BBC. In addition, due to natural allelic variations of the HIS1 gene in the sterile and restorer lines, some two-line hybrid sterile lines were sensitive to bTHs, and the corresponding restorers were resistant. We showed the potential effectiveness of using bTHs to address the issue of two-line hybrid rice seed purity stemming from the self-crossing of sterile lines during hybrid rice seed production. Finally, allelic variations of the HIS1 gene may also play an important role in the mechanized seed production of hybrid rice. CONCLUSIONS: Our findings offer guidance for the application of BBC in indica rice areas and provide a non-transgenic approach to address the seed purity issue of two-line hybrid rice.
BACKGROUND:Benzobicyclon (BBC) is a β-triketone herbicide (bTH) used in rice paddy fields. It has the advantages of high efficiency, low toxicity, high crop safety, and good environmental compatibility, and shows efficacy against paddy weeds resistant to other types of herbicides. However, as some important indica rice varieties are susceptible to BBC, BBC is currently only registered and applied in japonica rice cultivation areas. RESULTS: By analyzing haplotypes of the bTHs broad-spectrum resistance gene HIS1 and phenotypes for BBC in 493 major indica rice accessions in China, we identified a novel non-functional allelic variant of HIS1 in addition to the previously reported 28-bp deletion. Through detection with markers specific to the two non-functional mutations, it was clear that 25.4% of indica conventional varieties, 59.9% of fertility restorers, and 15.9% of sterile lines were susceptible to BBC. In addition, due to natural allelic variations of the HIS1 gene in the sterile and restorer lines, some two-line hybrid sterile lines were sensitive to bTHs, and the corresponding restorers were resistant. We showed the potential effectiveness of using bTHs to address the issue of two-line hybrid rice seed purity stemming from the self-crossing of sterile lines during hybrid rice seed production. Finally, allelic variations of the HIS1 gene may also play an important role in the mechanized seed production of hybrid rice. CONCLUSIONS: Our findings offer guidance for the application of BBC in indica rice areas and provide a non-transgenic approach to address the seed purity issue of two-line hybrid rice.
Authors: Renaud Beaudegnies; Andrew J F Edmunds; Torquil E M Fraser; Roger G Hall; Timothy R Hawkes; Glynn Mitchell; Juergen Schaetzer; Sebastian Wendeborn; Jane Wibley Journal: Bioorg Med Chem Date: 2009-03-14 Impact factor: 3.641
Authors: Ju Huang; Jing Li; Jun Zhou; Long Wang; Sihai Yang; Laurence D Hurst; Wen-Hsiung Li; Dacheng Tian Journal: Proc Natl Acad Sci U S A Date: 2018-07-23 Impact factor: 11.205
Authors: D Shoba; M Raveendran; S Manonmani; S Utharasu; D Dhivyapriya; G Subhasini; S Ramchandar; R Valarmathi; Nitasha Grover; S Gopala Krishnan; A K Singh; Pawan Jayaswal; Prashant Kale; M K Ramkumar; S V Amitha Mithra; T Mohapatra; Kuldeep Singh; N K Singh; N Sarla; M S Sheshshayee; M K Kar; S Robin; R P Sharma Journal: Rice (N Y) Date: 2017-04-04 Impact factor: 4.783