Yasamin Ghahramani1, Najmeh Mohammadi2, Ahmad Gholami3,4, Dordaneh Ghaffaripour2. 1. Department of Endodontics, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Department of Pediatric Dentistry, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
AIM: This study aimed to compare the antimicrobial efficacy of calcium hydroxide (CH) and triple antibiotic paste (TAP) against E. faecalis bacteria in infected primary molars. METHODS AND MATERIALS: Thirty-nine 4-6-year-old children with an infected primary molar were randomly divided into three equal groups (n = 13) to receive either CH or TAP and an untreated control group. Following access cavity preparation, the first microbiological samples (S1) were collected by using absorbent paper points. The canals were prepared and rinsed. Then, CH or TAP was applied in the root canals. Seven days later, the second microbiological samples (S2) were collected. DNA extraction was performed to count E. faecalis bacteria by using real-time PCR for S1 and S2 samples. Data were analyzed through one-way ANOVA and Tukey's test (α = 0.05). RESULTS: E. faecalis bacteria counts decreased significantly in CH and TAP groups compared with the control group (P ≤ 0.001). However, no statistically significant difference existed between these two groups (P=0.698). CONCLUSION: Both TAP and CH have significant antimicrobial effects as intracanal medicament between the treatment sessions in infected primary teeth.
AIM: This study aimed to compare the antimicrobial efficacy of calcium hydroxide (CH) and triple antibiotic paste (TAP) against E. faecalis bacteria in infected primary molars. METHODS AND MATERIALS: Thirty-nine 4-6-year-old children with an infected primary molar were randomly divided into three equal groups (n = 13) to receive either CH or TAP and an untreated control group. Following access cavity preparation, the first microbiological samples (S1) were collected by using absorbent paper points. The canals were prepared and rinsed. Then, CH or TAP was applied in the root canals. Seven days later, the second microbiological samples (S2) were collected. DNA extraction was performed to count E. faecalis bacteria by using real-time PCR for S1 and S2 samples. Data were analyzed through one-way ANOVA and Tukey's test (α = 0.05). RESULTS: E. faecalis bacteria counts decreased significantly in CH and TAP groups compared with the control group (P ≤ 0.001). However, no statistically significant difference existed between these two groups (P=0.698). CONCLUSION: Both TAP and CH have significant antimicrobial effects as intracanal medicament between the treatment sessions in infected primary teeth.
Authors: Juliana O Gondim; Juliana S Avaca-Crusca; Sandro R Valentini; Cleslei F Zanelli; Denise M P Spolidorio; Elisa M A Giro Journal: Int J Paediatr Dent Date: 2011-08-24 Impact factor: 3.455
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