Fabrication and Evaluation of 3D Printed Poly(l-lactide) Scaffold Functionalized with Quercetin-Polydopamine for Bone Tissue Engineering.

Quercetin (Qu), a bioflavonoid, has been reported to positively affect bone metabolism. For the first time, Qu with different concentrations was utilized to functionalize 3D-printed poly(l-lactide) (PLLA) scaffold with the aid of a polydopamine (PDA) layer through a convenient and effective way in this study. Results revealed that the coexistence of PDA and Qu can capacitate the 3D-printed PLLA scaffold to possess rougher surface, as well as better hydrophilicity and compressive properties. The resulting PDA- and Qu-modified PLLA scaffolds (Qu/PD-PLLA) can sustainably release Qu to some extent, which is more beneficial to the proliferation and attachment of MC3T3-E1 cells, upregulating ALP activity and calcium nodules as well as promoting the expression of related osteogenic genes and proteins. More significantly, such a positive impact of the Qu on the cell affinity and osteogenic activity played in a dose-dependent manner. This study revealed the potential of the 3D-printed Qu/PD-PLLA scaffolds with a certain amount of Qu as bone-repair materials.