Revathi Boyina1, Sujatha Dodoala2. 1. MLR Institute of Pharmacy, Department of Pharmacology, Hyderabad, India. 2. Sri Padmavati Mahila Visvavidyalayam, Institute of Pharmaceutical Technology, Department of Pharmacology, Tirupati, India.
Abstract
OBJECTIVES: The neurotoxic effects of food additives used in energy drinks have been investigated since the 1900s but safety concerns are rising and reassurance via safety testing in animals is demanded by the public. Rigorous safety testing is performed for dose optimisation and duration of treatment and to detect the methods to assess changes in mood and behaviour. Hence, we studied the neurobehavioral effects of selected food additives used in energy drinks and their combination in rats when consumed in high doses. MATERIALS AND METHODS: Young Sprague Dawley rats were divided into six groups. Group 1 was treated with the vehicle, group 2 was treated with 25 mg/kg p.o. caffeine, group 3 was treated with 5 mg/kg p.o. glucuronolactone, group 4 was treated with 8 mg/kg p.o. taurine, group 5 was treated with 84 mg/kg p.o. gluconolactone, and group 6 was treated with a combination of the three food additives. Neurobehavioral changes were evaluated on days 7, 14, and 21 using behavioural parameters. Neurobehavioral scoring and neurotransmitter estimation in rat brain tissue was performed on day 21. RESULTS: Significant changes were observed in the neurobehavioral parameters and neurobehavioural scoring in group 4 and group 6, compared with the control group (p<0.001). Furthermore, the significant decreases in neurotransmitter levels in the brains of rats that were treated with food additives indicated the neurotoxic effects of these substances. CONCLUSION: This study elaborated the neurobehavioral effects of selected food additives, namely glucuronolactone, taurine, and gluconolactone, when administered orally for 21 days in young rats. The highest toxic effects, including alterations in neurotransmitter levels, were observed in animals treated with a combination of food additives at high doses.
OBJECTIVES: The neurotoxic effects of food additives used in energy drinks have been investigated since the 1900s but safety concerns are rising and reassurance via safety testing in animals is demanded by the public. Rigorous safety testing is performed for dose optimisation and duration of treatment and to detect the methods to assess changes in mood and behaviour. Hence, we studied the neurobehavioral effects of selected food additives used in energy drinks and their combination in rats when consumed in high doses. MATERIALS AND METHODS: Young Sprague Dawley rats were divided into six groups. Group 1 was treated with the vehicle, group 2 was treated with 25 mg/kg p.o. caffeine, group 3 was treated with 5 mg/kg p.o. glucuronolactone, group 4 was treated with 8 mg/kg p.o. taurine, group 5 was treated with 84 mg/kg p.o. gluconolactone, and group 6 was treated with a combination of the three food additives. Neurobehavioral changes were evaluated on days 7, 14, and 21 using behavioural parameters. Neurobehavioral scoring and neurotransmitter estimation in rat brain tissue was performed on day 21. RESULTS: Significant changes were observed in the neurobehavioral parameters and neurobehavioural scoring in group 4 and group 6, compared with the control group (p<0.001). Furthermore, the significant decreases in neurotransmitter levels in the brains of rats that were treated with food additives indicated the neurotoxic effects of these substances. CONCLUSION: This study elaborated the neurobehavioral effects of selected food additives, namely glucuronolactone, taurine, and gluconolactone, when administered orally for 21 days in young rats. The highest toxic effects, including alterations in neurotransmitter levels, were observed in animals treated with a combination of food additives at high doses.
Entities:
Keywords:
Energy drinks; food additives; gluconolactone; glucuronolactone; taurine
Authors: Ken Takata; Huaxin Sheng; Cecil O Borel; Daniel T Laskowitz; David S Warner; Frederick W Lombard Journal: Exp Neurol Date: 2008-06-24 Impact factor: 5.330