Svitlana M Gubar1, Anna S Materiienko2, Nataliia M Smielova1, Liana G Budanova3, Victoriya A Georgiyants1. 1. National University of Pharmacy, Department of Pharmaceutical Chemistry, Kharkiv, Ukraine. 2. National University of Pharmacy, Department of Quality, Standardization and Certification of Medicines, Kharkiv, Ukraine. 3. National University of Pharmacy, Department of Foreign Languages, Kharkiv, Ukraine.
Abstract
OBJECTIVES: The aim of this study was the development a new, fully validated high performance liquid chromatography (HPLC) method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the herb Centaurium erythraea Rafn. The article describes a new approach to the standardization of C. erythraea and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by swertiamarin. MATERIALS AND METHODS: The quantitative determination of swertiamarin was performed in isocratic mode on a Symmetry C18 column using water and acetonitrile as solvents for the mobile phase. RESULTS: Validation characteristics of the developed method showed that it was linear in the whole range of concentrations from 0.01 mg/mL to 0.05 mg/mL swertiamarin. All validation characteristics met the established acceptance criteria. CONCLUSION: This method can be used in the standardization of raw materials, as well as in the analysis of medicinal products and dietary supplements that include C. erythraea. The established chromatographic method was successfully applied for the analysis of raw materials of C. erythraea with the quantitative content determination of swertiamarin in the analyzed samples.
OBJECTIVES: The aim of this study was the development a new, fully validated high performance liquid chromatography (HPLC) method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the herb Centaurium erythraea Rafn. The article describes a new approach to the standardization of C. erythraea and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by swertiamarin. MATERIALS AND METHODS: The quantitative determination of swertiamarin was performed in isocratic mode on a Symmetry C18 column using water and acetonitrile as solvents for the mobile phase. RESULTS: Validation characteristics of the developed method showed that it was linear in the whole range of concentrations from 0.01 mg/mL to 0.05 mg/mL swertiamarin. All validation characteristics met the established acceptance criteria. CONCLUSION: This method can be used in the standardization of raw materials, as well as in the analysis of medicinal products and dietary supplements that include C. erythraea. The established chromatographic method was successfully applied for the analysis of raw materials of C. erythraea with the quantitative content determination of swertiamarin in the analyzed samples.
Entities:
Keywords:
Common centaury herb; HPLC; method development; swertiamarin; validation
Authors: Patrícia Valentão; Paula B Andrade; Eurico Silva; Ana Vicente; Henrique Santos; M Lourdes Bastos; Rosa M Seabra Journal: J Agric Food Chem Date: 2002-01-30 Impact factor: 5.279