Yingjun Liu1,2,3,4, Yaying Song5,6, Peixi Liu1,2,3,4, Sichen Li1,2,3,4, Yuan Shi1,2,3,4, Guo Yu1,2,3,4, Kai Quan1,2,3,4, Zhiyuan Fan1,2,3,4, Peiliang Li1,2,3,4, Qingzhu An1,2,3,4, Wei Zhu1,2,3,4. 1. Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China. 2. Neurosurgical Institute of Fudan University, Shanghai, China. 3. Shanghai Clinical Medical Center of Neurosurgery. Shanghai, China. 4. Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, Shanghai, China. 5. Department of Neurology, Renji Hospital of Shanghai Jiao Tong University, Shanghai, China. 6. Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Abstract
AIMS: This study aimed to find critical proteins involved in the development of intracranial aneurysm by comparing proteomes of rabbit aneurysm model and human aneurysms. METHODS: Five human intracranial aneurysm samples and 5 superficial temporal artery samples, and 4 rabbit aneurysm samples and 4 control samples were collected for protein mass spectrometry. Four human intracranial aneurysm samples and 4 superficial temporal artery samples, and 6 rabbit aneurysm samples and 6 control samples were used for immunochemistry. RESULTS: Proteomic analysis revealed 180 significantly differentially expressed proteins in human intracranial aneurysms and 716 significantly differentially expressed proteins in rabbit aneurysms. Among them, 57 proteins were differentially expressed in both species, in which 24 were increased and 33 were decreased in aneurysms compared to the control groups. Proteins were involved in focal adhesion and extracellular matrix-receptor interaction pathways. We found that COL4A2, MYLK, VCL, and TAGLN may be related to aneurysm development. CONCLUSION: Proteomics analysis provided fundamental insights into the pathogenesis of aneurysm. Proteins related to focal adhesion and extracellular matrix-receptor interaction pathways play an important role in the occurrence and development of intracranial aneurysm.
AIMS: This study aimed to find critical proteins involved in the development of intracranial aneurysm by comparing proteomes of rabbit aneurysm model and human aneurysms. METHODS: Five human intracranial aneurysm samples and 5 superficial temporal artery samples, and 4 rabbit aneurysm samples and 4 control samples were collected for protein mass spectrometry. Four human intracranial aneurysm samples and 4 superficial temporal artery samples, and 6 rabbit aneurysm samples and 6 control samples were used for immunochemistry. RESULTS: Proteomic analysis revealed 180 significantly differentially expressed proteins in human intracranial aneurysms and 716 significantly differentially expressed proteins in rabbit aneurysms. Among them, 57 proteins were differentially expressed in both species, in which 24 were increased and 33 were decreased in aneurysms compared to the control groups. Proteins were involved in focal adhesion and extracellular matrix-receptor interaction pathways. We found that COL4A2, MYLK, VCL, and TAGLN may be related to aneurysm development. CONCLUSION: Proteomics analysis provided fundamental insights into the pathogenesis of aneurysm. Proteins related to focal adhesion and extracellular matrix-receptor interaction pathways play an important role in the occurrence and development of intracranial aneurysm.
Authors: Li Wang; Dong-chuan Guo; Jiumei Cao; Limin Gong; Kristine E Kamm; Ellen Regalado; Li Li; Sanjay Shete; Wei-Qi He; Min-Sheng Zhu; Stephan Offermanns; Dawna Gilchrist; John Elefteriades; James T Stull; Dianna M Milewicz Journal: Am J Hum Genet Date: 2010-11-04 Impact factor: 11.025
Authors: Juhana Frösen; Anna Piippo; Anders Paetau; Marko Kangasniemi; Mika Niemelä; Juha Hernesniemi; Juha Jääskeläinen Journal: Stroke Date: 2004-08-19 Impact factor: 7.914