Amina Boutellis1, Meriem Bellabidi2,3, Mohammed Hocine Benaissa4, Zoubir Harrat3, Karima Brahmi5, Rezak Drali6, Tahar Kernif7. 1. Laboratoire de Biodiversité Et Environnement: Interaction, Génomes, Faculté Des Sciences Biologiques, Université Des Sciences Et de La Technologie Houari Boumediene Bab Ezzouar, 16111, Algiers, Algeria. 2. Faculté Des Sciences de La Nature Et de La Vie, Laboratoire Des Bio Ressources Sahariennes, Université Kasdi Merbah Ouargla, 30000, Ouargla, Algeria. 3. Laboratoire D'Eco-Épidémiologie Parasitaire Et Génétique Des Populations, Institut Pasteur D'Algérie, 01 Rue du Petit Staouéli Dely-Brahim, 16302, Algiers, Algeria. 4. Centre de Recherche Scientifique Et Technique Sur Les Régions Arides (CRSTRA), 30002, Touggourt, Algeria. 5. Faculté Des Sciences Biologiques Et Des Sciences Agronomiques, Département de Biologie, Université Mouloud Mammeri, 15000, Tizi Ouzou, Algeria. 6. Plateforme Génomique, Bioinformatique, Institut Pasteur D'Algérie, 16302, Algiers, Algeria. 7. Laboratoire D'Eco-Épidémiologie Parasitaire Et Génétique Des Populations, Institut Pasteur D'Algérie, 01 Rue du Petit Staouéli Dely-Brahim, 16302, Algiers, Algeria. kernif.tahar@gmail.com.
Abstract
PURPOSE: Surra is a zoonotic disease caused by Trypanosoma evansi (Trypanozoon), a salivary trypanosome native to Africa which affects a wide range of mammals worldwide and causes mortality and significant economic loss. The present study was devoted to the molecular characterization of T. evansi derived from naturally infected dromedary camels in Algeria. METHODS: A total of 148 blood samples were collected from mixed age camels living in one of four geographic regions (Ouargla, El Oued, Biskra and Ghardaia) of Algeria. Samples underwent PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) complete sequence. RESULTS: DNA of Trypanosoma spp. was found in 19 camels (12.84%). Trypanosoma spp. molecular positivity was not affected by sex (p = 0.50), age (p = 0.08), or geographic location (p = 0.12). Based on multiple sequence alignment of the obtained DNA sequences with representative T. evansi ITS1 sequences available globally, the Algerian sequences were grouped within four different haplotypes including two which were original. CONCLUSION: Results of this study provide preliminary data on which future studies of genetic diversity and molecular epidemiology of T. evansi can be based.
PURPOSE: Surra is a zoonotic disease caused by Trypanosoma evansi (Trypanozoon), a salivary trypanosome native to Africa which affects a wide range of mammals worldwide and causes mortality and significant economic loss. The present study was devoted to the molecular characterization of T. evansi derived from naturally infecteddromedarycamels in Algeria. METHODS: A total of 148 blood samples were collected from mixed age camels living in one of four geographic regions (Ouargla, El Oued, Biskra and Ghardaia) of Algeria. Samples underwent PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) complete sequence. RESULTS: DNA of Trypanosoma spp. was found in 19 camels (12.84%). Trypanosoma spp. molecular positivity was not affected by sex (p = 0.50), age (p = 0.08), or geographic location (p = 0.12). Based on multiple sequence alignment of the obtained DNA sequences with representative T. evansi ITS1 sequences available globally, the Algerian sequences were grouped within four different haplotypes including two which were original. CONCLUSION: Results of this study provide preliminary data on which future studies of genetic diversity and molecular epidemiology of T. evansi can be based.
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