Aida Rasyidah Azman1,2, Naji Arafat Mahat3,4,5,6, Roswanira Abdul Wahab7,8, Wan Azlina Ahmad1, Jive Kasturi Puspanadan1,2, Mohamad Afiq Mohamed Huri1,2, Mohammad Rahim Kamaluddin9, Dzulkiflee Ismail10. 1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. 2. Enzyme Technology and Green Synthesis Research Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. 3. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. naji.arafat@utm.my. 4. Enzyme Technology and Green Synthesis Research Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. naji.arafat@utm.my. 5. Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. naji.arafat@utm.my. 6. Centre of Research For Fiqh Forensics and Judiciary, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia. naji.arafat@utm.my. 7. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. roswanira@utm.my. 8. Enzyme Technology and Green Synthesis Research Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia. roswanira@utm.my. 9. Human and Societal Well-being Research Centre, Faculty of Social Sciences and Humanities, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia. 10. Forensic Science Programme, School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
Abstract
OBJECTIVE: Optimisation of the green novel nanobio-based reagent (NBR) for rapid visualisation of groomed fingerprints on wet non-porous substrates using response surface methodology and assessment of its stability and sensitivity were attempted for forensic applications. RESULTS: Scanning electron microscopy images demonstrated successful attachments of NBR onto the constituents of fingerprints on the substrates. The highest average quality of visualised fingerprints was attained at the optimum condition (100 mg of CRL; 75 mg of acid-functionalised multi-walled carbon nanotubes; 5 h of immobilisation). The NBR produced comparable average quality of fingerprints with the commercially available small particle reagent, even after 4 weeks of storage (without any preservatives) in both chilled and sultry conditions. The NBR was sensitive enough to visualise the increasingly weaker fingerprints, particularly on glass slides. CONCLUSION: The optimised novel NBR could be the relatively greener option for visualising latent fingerprints on wet, non-porous substrates for forensic applications.
OBJECTIVE: Optimisation of the green novel nanobio-based reagent (NBR) for rapid visualisation of groomed fingerprints on wet non-porous substrates using response surface methodology and assessment of its stability and sensitivity were attempted for forensic applications. RESULTS: Scanning electron microscopy images demonstrated successful attachments of NBR onto the constituents of fingerprints on the substrates. The highest average quality of visualised fingerprints was attained at the optimum condition (100 mg of CRL; 75 mg of acid-functionalised multi-walled carbon nanotubes; 5 h of immobilisation). The NBR produced comparable average quality of fingerprints with the commercially available small particle reagent, even after 4 weeks of storage (without any preservatives) in both chilled and sultry conditions. The NBR was sensitive enough to visualise the increasingly weaker fingerprints, particularly on glass slides. CONCLUSION: The optimised novel NBR could be the relatively greener option for visualising latent fingerprints on wet, non-porous substrates for forensic applications.
Authors: S S Godipurge; S Yallappa; Naveen J Biradar; J S Biradar; B L Dhananjaya; Gajanan Hegde; K Jagadish; Gurumurthy Hegde Journal: Enzyme Microb Technol Date: 2016-08-10 Impact factor: 3.493
Authors: P Grochulski; Y Li; J D Schrag; F Bouthillier; P Smith; D Harrison; B Rubin; M Cygler Journal: J Biol Chem Date: 1993-06-15 Impact factor: 5.157