Literature DB >> 33382830

A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2.

Tomislav Maricic1, Olaf Nickel2, Ayinuer Aximu-Petri1, Elena Essel1, Marie Gansauge1, Philipp Kanis1, Dominik Macak1, Julia Richter1, Stephan Riesenberg1, Lukas Bokelmann1, Hugo Zeberg1,3, Matthias Meyer1, Stephan Borte2,4,5, Svante Pääbo1.   

Abstract

SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.

Entities:  

Year:  2020        PMID： 33382830     DOI： 10.1371/journal.pone.0244824

Source DB:  PubMed          Journal:  PLoS One        ISSN： 1932-6203            Impact factor:   3.240


  6 in total

Review 1.  Two Years into the COVID-19 Pandemic: Lessons Learned.

Authors:  Severino Jefferson Ribeiro da Silva; Jessica Catarine Frutuoso do Nascimento; Renata Pessôa Germano Mendes; Klarissa Miranda Guarines; Caroline Targino Alves da Silva; Poliana Gomes da Silva; Jurandy Júnior Ferraz de Magalhães; Justin R J Vigar; Abelardo Silva-Júnior; Alain Kohl; Keith Pardee; Lindomar Pena
Journal:  ACS Infect Dis       Date:  2022-08-08       Impact factor: 5.578

2.  The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes.

Authors:  Roberto Luraschi; Carlos Barrera-Avalos; Eva Vallejos-Vidal; Javiera Alarcón; Andrea Mella-Torres; Felipe Hernández; Ailen Inostroza-Molina; Daniel Valdés; Mónica Imarai; Claudio Acuña-Castillo; Felipe E Reyes-López; Ana María Sandino
Journal:  Can J Infect Dis Med Microbiol       Date:  2022-07-05       Impact factor: 2.585

Review 3.  Strategies for Scaling up SARS-CoV-2 Molecular Testing Capacity.

Authors:  Sanchita Das; Karen M Frank
Journal:  Clin Lab Med       Date:  2022-03-08       Impact factor: 2.172

4.  Usefulness of rapid antigen testing for SARS-CoV-2 screening of healthcare workers: a pilot study.

Authors:  Anja Šterbenc; Viktorija Tomič; Urška Bidovec Stojković; Katja Vrankar; Aleš Rozman; Mihaela Zidarn
Journal:  Clin Exp Med       Date:  2021-05-22       Impact factor: 5.057

5.  Sample Adequacy Control (SAC) Lowers False Negatives and Increases the Quality of Screening: Introduction of "Non-Competitive" SAC for qPCR Assays.

Authors:  Ivan Brukner; Alex Resendes; Shaun Eintracht; Andreas I Papadakis; Matthew Oughton
Journal:  Diagnostics (Basel)       Date:  2021-06-22

Review 6.  Obtaining Reliable RT-qPCR Results in Molecular Diagnostics-MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery.

Authors:  Christian Grätz; Maria L U Bui; Granit Thaqi; Benedikt Kirchner; Robert P Loewe; Michael W Pfaffl
Journal:  Life (Basel)       Date:  2022-03-07
  6 in total

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