Literature DB >> 33377020

Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.

Maria C Heinz1,2, Koen C Oost1,2, Hugo J G Snippert1,2.   

Abstract

Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology. For complete details on the use and execution of this protocol, please refer to Oost et al. (2018).
© 2020 The Author(s).

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Year:  2020        PMID： 33377020      PMCID： PMC7757012          DOI： 10.1016/j.xpro.2020.100126

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  23 in total

1.  Performance- and safety-enhanced lentiviral vectors containing the human interferon-beta scaffold attachment region and the chicken beta-globin insulator.

Authors:  Ali Ramezani; Teresa S Hawley; Robert G Hawley
Journal:  Blood       Date:  2003-02-13       Impact factor: 22.113

2.  Efficient genetic engineering of human intestinal organoids using electroporation.

Authors:  Masayuki Fujii; Mami Matano; Kosaku Nanki; Toshiro Sato
Journal:  Nat Protoc       Date:  2015-09-03       Impact factor: 13.491

3.  Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

Authors:  Toshiro Sato; Robert G Vries; Hugo J Snippert; Marc van de Wetering; Nick Barker; Daniel E Stange; Johan H van Es; Arie Abo; Pekka Kujala; Peter J Peters; Hans Clevers
Journal:  Nature       Date:  2009-03-29       Impact factor: 49.962

4.  Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.

Authors:  Toshiro Sato; Daniel E Stange; Marc Ferrante; Robert G J Vries; Johan H Van Es; Stieneke Van den Brink; Winan J Van Houdt; Apollo Pronk; Joost Van Gorp; Peter D Siersema; Hans Clevers
Journal:  Gastroenterology       Date:  2011-09-02       Impact factor: 22.682

5.  Reconstruction of the Human Colon Epithelium In Vivo.

Authors:  Shinya Sugimoto; Yuki Ohta; Masayuki Fujii; Mami Matano; Mariko Shimokawa; Kosaku Nanki; Shoichi Date; Shingo Nishikori; Yoshihiro Nakazato; Tetsuya Nakamura; Takanori Kanai; Toshiro Sato
Journal:  Cell Stem Cell       Date:  2017-12-28       Impact factor: 24.633

6.  In situ validation of an intestinal stem cell signature in colorectal cancer.

Authors:  Jennifer L Ziskin; Debra Dunlap; Murat Yaylaoglu; Imola K Fodor; William F Forrest; Rajesh Patel; Nianfeng Ge; Gordon G Hutchins; James K Pine; Philip Quirke; Hartmut Koeppen; Adrian M Jubb
Journal:  Gut       Date:  2012-05-25       Impact factor: 23.059

7.  Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells.

Authors:  Kazutaka Murata; Unmesh Jadhav; Shariq Madha; Johan van Es; Justin Dean; Alessia Cavazza; Kai Wucherpfennig; Franziska Michor; Hans Clevers; Ramesh A Shivdasani
Journal:  Cell Stem Cell       Date:  2020-02-20       Impact factor: 24.633

8.  A protocol for lentiviral transduction and downstream analysis of intestinal organoids.

Authors:  Jooske F Van Lidth de Jeude; Jacqueline L M Vermeulen; Paula S Montenegro-Miranda; Gijs R Van den Brink; Jarom Heijmans
Journal:  J Vis Exp       Date:  2015-04-20       Impact factor: 1.355

9.  Generation of BAC transgenic epithelial organoids.

Authors:  Gerald Schwank; Amanda Andersson-Rolf; Bon-Kyoung Koo; Nobuo Sasaki; Hans Clevers
Journal:  PLoS One       Date:  2013-10-18       Impact factor: 3.240

10.  Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer.

Authors:  Arianna Fumagalli; Koen C Oost; Lennart Kester; Jessica Morgner; Laura Bornes; Lotte Bruens; Lisa Spaargaren; Maria Azkanaz; Tim Schelfhorst; Evelyne Beerling; Maria C Heinz; Daniel Postrach; Danielle Seinstra; Anieta M Sieuwerts; John W M Martens; Stefan van der Elst; Martijn van Baalen; Debajit Bhowmick; Nienke Vrisekoop; Saskia I J Ellenbroek; Saskia J E Suijkerbuijk; Hugo J Snippert; Jacco van Rheenen
Journal:  Cell Stem Cell       Date:  2020-03-12       Impact factor: 24.633
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  1 in total

1.  Generation of mixed murine organoids to model cellular interactions.

Authors:  Ana Krotenberg Garcia; Jacco van Rheenen; Saskia Jacoba Elisabeth Suijkerbuijk
Journal:  STAR Protoc       Date:  2021-12-08
  1 in total

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