| Literature DB >> 33367996 |
Hong Yang1,2, Feng Xiong1,2, Yi-Ge Song1,2, Hai-Fei Jiang1,2, Hai-Bin Qin1,2, Jing Zhou1,2, Sha Lu3, Steven F Grieco4, Xiangmin Xu4, Wen-Bo Zeng5, Fei Zhao6,7, Min-Hua Luo8,9,10.
Abstract
Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1 (HSV-1) strain H129 (H129), and have been successfully applied to map diverse neural circuits. Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.Entities:
Keywords: Anterograde transneuronal tracer; Application; HSV-1 strain H129 (H129); Monosynaptic tracer; Neural circuit; Neural circuit tracing; Polysynaptic tracer; Production
Mesh：
Year: 2020 PMID： 33367996 PMCID： PMC8099975 DOI： 10.1007/s12264-020-00614-3
Source DB: PubMed Journal: Neurosci Bull ISSN： 1995-8218 Impact factor: 5.203