Literature DB >> 3336364

Production and characterization of biologically active recombinant beta nerve growth factor.

F Hallböök1, T Ebendal, H Persson.   

Abstract

DNA fragments encoding either rat or chicken beta nerve growth factor (NGF) were inserted in the expression vector p91023(B) for transient expression in COS cells. The two NGF constructs produced RNA transcripts and proteins of the predicted sizes. Conditioned media from the transfected cells stimulated neurite outgrowth from cultured chicken embryo sympathetic ganglia. The results show that the rat or chicken NGF gene can direct the synthesis of a biologically active NGF protein after transfection of COS cells.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3336364      PMCID： PMC363148          DOI： 10.1128/mcb.8.1.452-456.1988

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  23 in total

1.  Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.

Authors:  N J Proudfoot; G G Brownlee
Journal:  Nature       Date:  1974-11-29       Impact factor: 49.962

2.  NGF-mediated increase of choline acetyltransferase (ChAT) in the neonatal rat forebrain: evidence for a physiological role of NGF in the brain?

Authors:  H Gnahn; F Hefti; R Heumann; M E Schwab; H Thoenen
Journal:  Brain Res       Date:  1983-07       Impact factor: 3.252

3.  SV40-transformed simian cells support the replication of early SV40 mutants.

Authors:  Y Gluzman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

4.  Chronic intraventricular injections of nerve growth factor elevate hippocampal choline acetyltransferase activity in adult rats with partial septo-hippocampal lesions.

Authors:  F Hefti; A Dravid; J Hartikka
Journal:  Brain Res       Date:  1984-02-20       Impact factor: 3.252

5.  Identification of the components necessary for adenovirus translational control and their utilization in cDNA expression vectors.

Authors:  R J Kaufman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

6.  Nerve growth factor treatment after brain injury prevents neuronal death.

Authors:  L F Kromer
Journal:  Science       Date:  1987-01-09       Impact factor: 47.728

7.  Human beta-nerve growth factor gene sequence highly homologous to that of mouse.

Authors:  A Ullrich; A Gray; C Berman; T J Dull
Journal:  Nature       Date:  1983-06-30       Impact factor: 49.962

8.  Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

Authors:  G G Wong; J S Witek; P A Temple; K M Wilkens; A C Leary; D P Luxenberg; S S Jones; E L Brown; R M Kay; E C Orr
Journal:  Science       Date:  1985-05-17       Impact factor: 47.728

9.  Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor.

Authors:  J Scott; M Selby; M Urdea; M Quiroga; G I Bell; W J Rutter
Journal:  Nature       Date:  1983-04-07       Impact factor: 49.962

10.  Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation.

Authors:  S Korsching; G Auburger; R Heumann; J Scott; H Thoenen
Journal:  EMBO J       Date:  1985-06       Impact factor: 11.598
View more
  6 in total

1.  Prohormone processing in Xenopus oocytes: characterization of cleavage signals and cleavage enzymes.

Authors:  J Korner; J Chun; L O'Bryan; R Axel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

2.  NGF gene expression in dividing and non-dividing cells from AAV-derived constructs.

Authors:  S Wang; W J Millard; E M Meyer
Journal:  Neurochem Res       Date:  1998-05       Impact factor: 3.996

3.  Soluble Expression of Recombinant Nerve Growth Factor in Cytoplasm of Escherichia coli.

Authors:  Shabnam Shamriz; Hamideh Ofoghi; Zahra Amini-Bayat
Journal:  Iran J Biotechnol       Date:  2016-03       Impact factor: 1.671

4.  Molecular cloning and neurotrophic activities of a protein with structural similarities to nerve growth factor: developmental and topographical expression in the brain.

Authors:  P Ernfors; C F Ibáñez; T Ebendal; L Olson; H Persson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

5.  Two conserved domains in the NGF propeptide are necessary and sufficient for the biosynthesis of correctly processed and biologically active NGF.

Authors:  U Suter; J V Heymach; E M Shooter
Journal:  EMBO J       Date:  1991-09       Impact factor: 11.598

6.  Structure-function studies of nerve growth factor: functional importance of highly conserved amino acid residues.

Authors:  C F Ibáñez; F Hallböök; T Ebendal; H Persson
Journal:  EMBO J       Date:  1990-05       Impact factor: 11.598
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.