Literature DB >> 33361274

High-resolution scanning electron microscopy for the analysis of three-dimensional ultrastructure of clots in acute ischemic stroke.

Oana Madalina Mereuta1,2, Seán Fitzgerald3,2, Trace A Christensen4, Adam L Jaspersen4, Daying Dai3, Mehdi Abbasi3, Tejaswini Puttappa3, Ram Kadirvel3, David F Kallmes3,5, Karen M Doyle2, Waleed Brinjikji3,5.   

Abstract

BACKGROUND: Characterization of acute ischemic stroke (AIS) clots has typically focused on two-dimensional histological analysis of the thrombus. The three-dimensional (3D) architecture and distribution of components within emboli have not been fully investigated. The aim of this study was to examine the composition and microstructure of AIS clots using histology and serial block-face scanning electron microscopy (SBFSEM).
METHODS: As part of the multi-institutional STRIP registry, 10 consecutive AIS emboli were collected from 10 patients treated by mechanical thrombectomy. Histological and immunohistochemical analysis was performed to determine clot composition. SBFSEM was used to assess the ultrastructural organization of the clots and specific features of individual components.
RESULTS: Quantification of Martius Scarlett Blue stain identified fibrin (44.4%) and red blood cells (RBCs, 32.6%) as the main components. Immunohistochemistry showed a mean platelet and von Willebrand factor content of 23.9% and 11.8%, respectively. The 3D organization of emboli varied greatly depending on the region analyzed. RBC-rich areas were composed mainly of tightly packed RBCs deformed into polyhedrocytes with scant fibrin fibers interwoven between cells. The regions with mixed composition showed thick fibrin fibers along with platelets, white blood cells and RBC clusters. Fibrin-rich areas contained dense fibrin masses with sparse RBC. In three cases, the fibrin formed a grid-like or a sponge-like pattern, likely due to thrombolytic treatment. Segmentation showed that fibrin fibers were thinner and less densely packed in these cases.
CONCLUSIONS: 3D-SEM provides novel and potentially clinically relevant information on clot components and ultrastructure which may help to inform thrombolytic treatment and medical device design. © Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.

Entities:  

Keywords:  embolic; stroke; technology; thrombectomy; thrombolysis

Mesh：

Year:  2020        PMID： 33361274      PMCID： PMC8226367          DOI： 10.1136/neurintsurg-2020-016709

Source DB:  PubMed          Journal:  J Neurointerv Surg        ISSN： 1759-8478            Impact factor:   8.572


  24 in total

1.  Reconstruct: a free editor for serial section microscopy.

Authors:  J C Fiala
Journal:  J Microsc       Date:  2005-04       Impact factor: 1.758

Review 2.  Correlation of imaging and histopathology of thrombi in acute ischemic stroke with etiology and outcome: a systematic review.

Authors:  Waleed Brinjikji; Sharon Duffy; Anthony Burrows; Werner Hacke; David Liebeskind; Charles B L M Majoie; Diederik W J Dippel; Adnan H Siddiqui; Pooja Khatri; Blaise Baxter; Raul Nogeuira; Matt Gounis; Tudor Jovin; David F Kallmes
Journal:  J Neurointerv Surg       Date:  2016-05-10       Impact factor: 5.836

3.  The Blood And Clot Thrombectomy Registry And Collaboration (BACTRAC) protocol: novel method for evaluating human stroke.

Authors:  Justin F Fraser; Lisa A Collier; Amy A Gorman; Sarah R Martha; Kathleen E Salmeron; Amanda L Trout; Danielle N Edwards; Stephanie M Davis; Douglas E Lukins; Abdulnasser Alhajeri; Stephen Grupke; Jill M Roberts; Gregory J Bix; Keith R Pennypacker
Journal:  J Neurointerv Surg       Date:  2018-07-31       Impact factor: 5.836

4.  Mechanical behavior of in vitro blood clots and the implications for acute ischemic stroke treatment.

Authors:  Sarah Johnson; Juyu Chueh; Matthew J Gounis; Ray McCarthy; J Patrick McGarry; Peter E McHugh; Michael Gilvarry
Journal:  J Neurointerv Surg       Date:  2019-11-28       Impact factor: 5.836

5.  Erythrocyte damage and leukocyte activation in ischemic stroke.

Authors:  Alice Santos-Silva; Irene Rebelo; Elisabeth Castro; Luís Belo; Cristina Catarino; Isabel Monteiro; Maria Daniel Almeida; Alexandre Quintanilha
Journal:  Clin Chim Acta       Date:  2002-06       Impact factor: 3.786

6.  Iron enhances generation of fibrin fibers in human blood: implications for pathogenesis of stroke.

Authors:  Boguslaw Lipinski; Etheresia Pretorius; Hester Magdalena Oberholzer; Wendy Jeanette Van Der Spuy
Journal:  Microsc Res Tech       Date:  2012-04-05       Impact factor: 2.769

7.  Platelet-rich clots as identified by Martius Scarlet Blue staining are isodense on NCCT.

Authors:  Sean T Fitzgerald; Shunli Wang; Daying Dai; Andrew Douglas; Ramanathan Kadirvel; Matthew J Gounis; Juyu Chueh; Ajit S Puri; Kennith F Layton; Ike C Thacker; Ricardo A Hanel; Eric Sauvageau; Amin Aghaebrahim; Mohammed A Almekhlafi; Andrew M Demchuk; Raul G Nogueira; Vitor M Pereira; Peter Kvamme; Yasha Kayan; Josser E Delgado Almandoz; Albert J Yoo; David F Kallmes; Karen M Doyle; Waleed Brinjikji
Journal:  J Neurointerv Surg       Date:  2019-04-05       Impact factor: 5.836

8.  Contraction of Blood Clots Is Impaired in Acute Ischemic Stroke.

Authors:  Valerie Tutwiler; Alina D Peshkova; Izabella A Andrianova; Dina R Khasanova; John W Weisel; Rustem I Litvinov
Journal:  Arterioscler Thromb Vasc Biol       Date:  2016-12-01       Impact factor: 8.311

9.  Structural analysis of ischemic stroke thrombi: histological indications for therapy resistance.

Authors:  Senna Staessens; Frederik Denorme; Olivier Francois; Linda Desender; Tom Dewaele; Peter Vanacker; Hans Deckmyn; Karen Vanhoorelbeke; Tommy Andersson; Simon F De Meyer
Journal:  Haematologica       Date:  2020-01-31       Impact factor: 9.941

10.  The distinctive structure and composition of arterial and venous thrombi and pulmonary emboli.

Authors:  Irina N Chernysh; Chandrasekaran Nagaswami; Sofia Kosolapova; Alina D Peshkova; Adam Cuker; Douglas B Cines; Carolyn L Cambor; Rustem I Litvinov; John W Weisel
Journal:  Sci Rep       Date:  2020-03-20       Impact factor: 4.379
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  7 in total

Review 1.  Composition and Organization of Acute Ischemic Stroke Thrombus: A Wealth of Information for Future Thrombolytic Strategies.

Authors:  Jean-Philippe Desilles; Lucas Di Meglio; Francois Delvoye; Benjamin Maïer; Michel Piotin; Benoît Ho-Tin-Noé; Mikael Mazighi
Journal:  Front Neurol       Date:  2022-07-06       Impact factor: 4.086

2.  Hemostasis Disturbances in Continuous-Flow Left Ventricular Assist Device (CF-LVAD) Patients-Rationale and Study Design.

Authors:  Kuczaj Agnieszka; Hudzik Bartosz; Kaczmarski Jacek; Przybyłowski Piotr
Journal:  J Clin Med       Date:  2022-06-27       Impact factor: 4.964

3.  Association between clot composition and stroke origin in mechanical thrombectomy patients: analysis of the Stroke Thromboembolism Registry of Imaging and Pathology.

Authors:  Waleed Brinjikji; Mehdi Abbasi; Raul G Nogueira; Peter Kvamme; Kennith F Layton; Josser E Delgado Almandoz; Ricardo A Hanel; Vitor Mendes Pereira; Mohammed A Almekhlafi; Albert J Yoo; Babak S Jahromi; Matthew J Gounis; Biraj Patel; Seán Fitzgerald; Oana Madalina Mereuta; Daying Dai; Ramanathan Kadirvel; Karen Doyle; Luis Savastano; Harry J Cloft; Diogo C Haussen; Alhamza R Al-Bayati; Mahmoud H Mohammaden; Leonardo Pisani; Gabriel Martins Rodrigues; Ike C Thacker; Yasha Kayan; Alexander Copelan; Amin Aghaebrahim; Eric Sauvageau; Andrew M Demchuk; Parita Bhuva; Jazba Soomro; Pouya Nazari; Donald Robert Cantrell; Ajit S Puri; John Entwistle; Eric C Polley; David F Kallmes
Journal:  J Neurointerv Surg       Date:  2021-03-15       Impact factor: 8.572

Review 4.  Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.

Authors:  Chudai Zeng; Zhuohui Chen; Haojun Yang; Yishu Fan; Lujing Fei; Xinghang Chen; Mengqi Zhang
Journal:  Int J Biol Sci       Date:  2022-01-01       Impact factor: 6.580

5.  Does prior administration of rtPA influence acute ischemic stroke clot composition? Findings from the analysis of clots retrieved with mechanical thrombectomy from the RESTORE registry.

Authors:  Rosanna Rossi; Sara Molina; Oana Madalina Mereuta; Andrew Douglas; Seán Fitzgerald; Ciara Tierney; Abhay Pandit; Paul Brennan; Sarah Power; Alan O'Hare; Michael Gilvarry; Ray McCarthy; Georgios Magoufis; Georgios Tsivgoulis; András Nagy; Ágnes Vadász; Katarina Jood; Petra Redfors; Annika Nordanstig; Erik Ceder; Dennis Dunker; Jeanette Carlqvist; Klearchos Psychogios; István Szikora; Turgut Tatlisumak; Alexandros Rentzos; John Thornton; Karen M Doyle
Journal:  J Neurol       Date:  2021-08-20       Impact factor: 4.849

6.  Histological evaluation of acute ischemic stroke thrombi may indicate the occurrence of vessel wall injury during mechanical thrombectomy.

Authors:  Oana Madalina Mereuta; Mehdi Abbasi; Seán Fitzgerald; Daying Dai; Ram Kadirvel; Ricardo A Hanel; Albert J Yoo; Mohammed A Almekhlafi; Kennith F Layton; Josser E Delgado Almandoz; Peter Kvamme; Vitor Mendes Pereira; Babak S Jahromi; Raul G Nogueira; Matthew J Gounis; Biraj Patel; Amin Aghaebrahim; Eric Sauvageau; Parita Bhuva; Jazba Soomro; Andrew M Demchuk; Ike C Thacker; Yasha Kayan; Alexander Copelan; Pouya Nazari; Donald Robert Cantrell; Diogo C Haussen; Alhamza R Al-Bayati; Mahmoud Mohammaden; Leonardo Pisani; Gabriel Martins Rodrigues; Ajit S Puri; John Entwistle; Alexander Meves; Jorge L Arturo Larco; Luis Savastano; Harry J Cloft; David F Kallmes; Karen M Doyle; Waleed Brinjikji
Journal:  J Neurointerv Surg       Date:  2021-05-11       Impact factor: 5.836

Review 7.  Mechanisms of fibrinolysis resistance and potential targets for thrombolysis in acute ischaemic stroke: lessons from retrieved stroke emboli.

Authors:  Waleed Brinjikji; Oana Madalina Mereuta; Daying Dai; David F Kallmes; Luis Savastano; Yang Liu; Shahid M Nimjee; Raul G Nogueira; Mehdi Abbasi; Ramanathan Kadirvel
Journal:  Stroke Vasc Neurol       Date:  2021-07-26
  7 in total

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