Edyta B Hendiger1,2, Marcin Padzik3, Agnieszka Żochowska3, Wanda Baltaza3, Gabriela Olędzka3, Diana Zyskowska3, Julita Bluszcz3, Sylwia Jarzynka3, Lidia Chomicz3, Marta Grodzik4, Jacek Hendiger5, José E Piñero6, Jarosław Grobelny7, Katarzyna Ranoszek-Soliwoda7, Jacob Lorenzo-Morales6. 1. Laboratory of Parasitology, Department of Medical Biology, Medical University of Warsaw, 14/16 Litewska Street, 00-575, Warsaw, Poland. edyta.hendiger@wum.edu.pl. 2. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez S/N, 38203, Tenerife, Spain. edyta.hendiger@wum.edu.pl. 3. Laboratory of Parasitology, Department of Medical Biology, Medical University of Warsaw, 14/16 Litewska Street, 00-575, Warsaw, Poland. 4. Department of Nanobiotechnology and Experimental Ecology, Institute of Biology, Warsaw, University of Life Sciences, 8 Ciszewskiego Street, 02-787, Warsaw, Poland. 5. Faculty of Building Services, Hydro and Environmental Engineering, Warsaw University of Technology, 20 Nowowiejska Street, 00-653, Warsaw, Poland. 6. Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez S/N, 38203, Tenerife, Spain. 7. Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, 163 Pomorska Street, 90-236, Lodz, Poland.
Abstract
BACKGROUND: Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthesized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions. METHODS: The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance. RESULTS: We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recommended by the manufacturers. CONCLUSIONS: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users.
BACKGROUND: Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthesized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions. METHODS: The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance. RESULTS: We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recommended by the manufacturers. CONCLUSIONS: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users.
Authors: Marcin Padzik; Edyta B Hendiger; Agnieszka Żochowska; Jarosław Szczepaniak; Wanda Baltaza; Anna Pietruczuk-Padzik; Gabriela Olędzka; Lidia Chomicz Journal: Ann Agric Environ Med Date: 2019-03-13 Impact factor: 1.447
Authors: Sara González-Fernández; Victor Lozano-Iturbe; Mª Fe Menéndez; Helena Ordiales; Iván Fernández-Vega; Jesús Merayo; Fernando Vazquez; Luis M Quirós; Carla Martín Journal: Antibiotics (Basel) Date: 2022-08-03
Authors: Edyta B Hendiger; Marcin Padzik; Inés Sifaoui; María Reyes-Batlle; Atteneri López-Arencibia; Diana Zyskowska; Marta Grodzik; Anna Pietruczuk-Padzik; Jacek Hendiger; Gabriela Olędzka; Lidia Chomicz; José E Piñero; Jacob Lorenzo-Morales Journal: Pathogens Date: 2021-05-11