Viscoelastic coagulation test for liver transplantation.

Coagulation and transfusion management in patients undergoing liver transplantation is challenging. Proper perioperative monitoring of hemostasis is essential to predict the risk of bleeding during surgery, to detect potential causes of hemorrhage in time, and to guide hemostatic therapy. The value of conventional coagulation test is questionable in the acute perioperative setting due to their long turnaround time and the inability to adequately reflect the complex changes in hemostasis in patients with liver disease. Viscoelastic coagulation tests provide simultaneous measurement of multiple aspects of whole-blood coagulation including plasmatic coagulation and fibrinolytic factors and inhibitors that reflect most aspects of hemostasis. Coagulation initiation, mechanical clot stability, and fibrinolysis can be estimated immediately using point-of-care techniques. Therefore, viscoelastic coagulation tests including ROTEM & TEG would be useful to guide patient blood management strategy during liver transplantation.

INTRODUCTION

As the liver plays an important role in the blood coagulation system, hepatic dysfunction could lead to coagulopathy. Even during hepatic failure, the coagulation system can maintain a balance via various mechanisms, but it is not stable. Most patients undergoing liver transplantation experience severe changes in blood coagulation status during surgery that results in bleeding tendency. Because excessive bleeding and blood transfusion during liver transplantations are risk factors for patients’ poor outcomes [1], it is important to rapidly perceive and manage the changes of the blood coagulation status to prevent excessive bleeding and reduce blood transfusion during surgery. Conventional coagulation test (CCT) to diagnose coagulation disorders includes tests for bleeding time (BT), prothrombin time (PT), activated partial prothrombin time (aPTT), thrombin time (TT), platelet count, and fibrinogen. However, studies have reported that it is difficult to predict the possibility of bleeding or blood transfusion with CCT [2-4] and that CCT is insufficient to distinguish whether a coagulopathy has occurred [5,6]. Furthermore, CCT has the disadvantage of being time-consuming, and hence, it cannot be applied in situations that require rapid judgment and treatment. Recently, viscoelastic coagulation test has been garnering attention as a method that can overcome these limitations [7]. This test was developed in 1948 and approved for use in enabling targeted treatment by distinguishing deficient coagulation factors in patients with coagulation factor deficiencies. Since the 1960s, cases of perioperative applications have been reported. Viscoelastic coagulation test is a method to determine coagulation in real-time by obtaining the thromboelastrography while the blood coagulation is in process. It is reported that this test can provide a more comprehensive and accurate information about coagulation function than conventional tests [8]. Other than the platelet count, most CCTs are plasma-based tests and can only reflect the initial stages of the coagulation. In contrast, viscoelastic coagulation tests are plasma and cell-based tests using the whole blood and can provide information from coagulation initiation to fibrinolysis, the strength and stability of the clot. Therefore, it provides a real time accurate biological representation of coagulation [6] and can be used as a point-of-care testing. The viscoelastic coagulation test is often applied to patients undergoing surgery for trauma in which bleeding can be problematic, and various studies on its utility has been conducted. And there are many studies have been conducted on its use during liver transplantation recently. Therefore, this review article aims to examine the usefulness of the viscoelastic coagulation test and its application in liver transplantation.

VISCOELASTIC COAGULATION TEST

Viscoelastic coagulation test is a method that measures, digitalizes, and graphically plots the viscoelasticity generated between the fibrin strands and the platelets in the process of blood coagulation. There are currently two types of equipment that can be used for viscoelastic coagulation test: classical thrombelastography (TEG, Haemonetics, USA) and rotational thromboelastometry (ROTEM, Tem International GmbH, Germany). The parameter that can be obtained first through these tests is the clotting time (clotting time or CT in ROTEM; reaction time or R in TEG) which is the time it takes until the graph amplitude reaches 2 mm. This can be considered an index of the coagulation factor function. Delay in this parameter can signal coagulation factor deficiency or dysfunction. The next parameter is the amount of time it takes for the amplitude to increase from 2 mm to 20 mm which is referred to as the clot formation time (clot formation time or CFT in ROTEM; κ in TEG), and this reflects fibrin formation. Platelet response is considered to start at this time, and the slope of the graph until this point is represented as the α angle. Then, the amplitude gradually increases and reaches maximum. This maximum amplitude (maximum clot firmness or MCF in ROTEM; maximum amplitude or MA in TEG) is an index that reflects the strength of the clot. To quickly assess the strength of the clot, estimates can be made from the amplitudes measured at the 5, 10, and 20 min after the clotting time. The final parameter represents the fibrinolysis. The amplitude 30 min or 60 min after clotting time is calculated as a percentage of the maximum amplitude (LI30, LI60 in ROTEM; LY30, LY60 in TEG) to represent the stability of the clot (Fig. 1). Because the activators used in the two equipment are slightly different, the reference ranges are different.

Fig. 1.

Viscoelastic coagulation test using whole blood. CT: clotting time, R: reaction time, CFT: clot formation time, MCF: maximum clot firmness, MA: maximum amplitude.

The types of tests that can be performed with TEG include Native and Kaolin TEG as the basic tests, Rapid TEG which enables quick response by inducing responses with the tissue factors, heparinase method that shows results by removing the effects of heparin, and functional fibrinogen (FF) which shows the fibrin functions. In addition, the platelet function test is added. The tests that can be performed with ROTEM include NATEM which is a basic test that adds calcium to the citrated blood. The remaining tests can be classified into two types: EXTEM group and INTEM group. EXTEM is a method of making the response speed faster by activating the coagulation process using the tissue factors. Through this method, the extrinsic pathway of the coagulation can be identified. By adding aprotinin to this test and inhibiting fibrinolysis, APTEM can be obtained. By adding cytochalasin D and inhibiting platelet functions, FIBTEM can be performed. The other family of tests is INTEM by which the intrinsic pathway of the blood coagulation can be identified by adding ellagic acid. HEPTEM can be performed by adding heparinase and neutralizing the effect of heparin (Table 1). A test that can separately assess the platelet coagulation is soon to be commercialized. Both TEG and ROTEM were previously used by putting the blood in the testing cup and attaching it to the machine, the convenience of testing has increased presently with the introduction of the cartridge-type models.

Table 1.

Description of ROTEM & TEG Assays

Test		Description
ROTEM
NATEM		Native whole blood sample analyzed following only recalcification
		Impractical for clinical use given long CFT time
EXTEM		Tissue factor activation: reagent contains tissue factor as an activator and provides information similar to that of the PT
	APTEM	Contains aprotinin for inhibiting fibrinolysis; used in conjunction with EXTEM reagent and compared to EXTEM analysis to assess fibrinolysis
	FIBTEM	Utilizes cytochalasin D, an actin polymerization inhibitor to exclude the platelet contribution.
		When compared to EXTEM analysis, allows qualitative analysis of the fibrinogen contribution to clot strength
INTEM		Contact activation: Reagent contains phospholipid and ellagic acid as activators and provides information similar to that of the APTT
	HEPTEM	Contains lyophilized heparinase for neutralizing unfractionated heparin; used in conjunction with INTEM reagent and compared to ITEM analysis to assess heparin effect
TEG
Kaolin		An intrinsic pathway activated assay identifies underlying hemostatic characteristics and risk of bleeding or thrombosis
Kaolin with heparinase		Eliminates the effect of heparin in the test sample, and used in conjunction with Kaolin assess the presence of systemic heparin
Rapid TEG™		An intrinsic and extrinsic pathway activated assay speeds the coagulation process to more rapidly assess coagulation properties
Functional fibrinogen		Used in conjunction with Kaolin or Rapid TEG assess relative contribution of platelets and fibrin to overall clot strength.

CFT: clot formation time, PT: prothrombin time, aPTT: activated partial prothrombin time.

Although the reference ranges of clotting time varies between testing equipment and method, plasma coagulation factor deficiency can generally be assessed within 5 min. Abnormal clot strength can be assessed within 25 min if the values at 5, 10, or 20 min after clotting time are used before the maximum amplitude is observed. Additionally, by interpreting the amplitude results of FIBTEM or FF together, we could discriminate between the fibrin and platelet function or quantity problems. Although it can take more than one hour to completely assess the state of fibrinolysis, prediction can be made within 35 min if the abnormal fibrinolysis is severe by referencing the values at 30 min after the clotting time. In other words, using the viscoelastic coagulation test enables the assessment of the urgent need for fresh frozen plasma within 5 min, the need for fibrin or platelet supplement other than the coagulation factors and the risk of bleeding within 25 min.

NECESSITY AND ADVANTAGES OF VISCOELASTIC COAGULATION TEST FOR LIVER TRANSPLANTATION

Because patients undergoing liver transplantation have severely reduced liver functions, they are unable to generate sufficient amounts of vitamin K-dependent procoagulants, protein C, and protein S, which play important roles in coagulation. Furthermore, they simultaneously show decreased alpha-2-antiplasmin, thrombin activatable fibrinolysis inhibitor (TAFI), and plasminogen and increased tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) which must be removed by the liver. Besides deficiency in liver functions, decrease in platelet count and its functions can be observed due to spleen enlargement and bone marrow suppression in patients with liver diseases. In addition, decrease in fibrinogen and its functions are common. These changes in the coagulation-related substances maintain an equilibrium but are unstable. Therefore, coagulopathy disorders and the resulting worsening of bleeding during liver transplantation is common. Because pre-operative CCT only reflects the pro-coagulant aspects of the plasma, it cannot provide accurate information, and the results are not related to the volume of bleeding or the transfusion of blood products during liver transplantation. Thus, it is difficult to predict bleeding or the need for blood transfusion [9, 10]. Furthermore, because there are severe changes in the state of coagulation among the stages of surgery during liver transplantation, perioperative real-time monitoring is more important than the results from pre-operative tests. CCT requires the process of obtaining blood and sending it to a diagnostic lab, and generally 45–90 min are required to obtain the results. Therefore, CCT is not suitable for point-of-care testing during liver transplantation. In contrast, the viscoelastic coagulation test enables point-of-care testing. It can lessen the burden of sending the blood to the lab, as long as the testing equipment is available, and generally 15–25 min are sufficient to obtain the main results for treatment. Therefore, it enables real-time monitoring and goal-directed therapy [11]. Although it can take 60–90 min to obtain all results, only few minutes are necessary to predict an immediate coagulopathy and clinically assess the need for treatment and the type of treatment. Many studies suggest that the viscoelastic coagulation test is more sensitive to coagulation disorders than CCT [12,13]. Moreover, in vivo studies on liver transplantation showed that pre-operative ROTEM test results are good predictors of the need for blood transfusion, particularly the need for fresh frozen plasma blood transfusion [14]. A retrospective analysis of patients undergoing liver transplantation reported that the MCF was strongly correlated with platelet count and fibrin concentration, and thus, can replace conventional tests [15]. In particular, because the amplitude obtained 5 or 10 min after the clotting time reflect the decrease in platelet or fibrin concentration, a quick assessment of the clot strength is possible [16]. Additionally, the viscoelastic test showed better results than CCT in predicting bleeding after the liver transplantation due to pathologic coagulopathy [17]. Thus, viscoelastic test is often used to test the coagulation function of the patients undergoing liver transplantation. It was reported in the 1980s by the University of Pittsburgh that blood transfusion can be decreased by applying TEG during liver transplantation [18]. A decrease in the transfusion of fresh frozen plasma by applying the transfusion strategy using this viscoelastic coagulation test for liver transplantation was also reported recently [19]. Because this test enables the immediate assessment of the state of fibrinolysis which cannot be obtained through CCT, it is suggested that transfusion may be decreased if the viscoelastic coagulation test is applied for liver transplantation where hyperfibrinolysis commonly occurs [20].

Depending on the type of the liver diseases that caused the hepatic failure, the state of coagulopathy may differ among patients undergoing liver transplantation. For example, patients with hepatocellular carcinoma, cholestatic hepatitis, and non-alcoholic steatohepatitis (NASH) show a relative hypercoagulation. In these patients, thrombosis is as important as bleeding. Although the prevalence of thrombosis is not high in Korea, liver disease and liver transplantation are clear risk factors for thromboembolism. Even if there is a bleeding tendency due to a coagulopathy, the risk of thrombosis does not decrease [21]. Furthermore, patients undergoing organ transplantation have a risk of fibrinolytic shutdown [22], and this is reported to increase the risk of thrombosis and bleeding during surgery. Krzanicki et al. [23] showed that many patients suffer from hypercoagulation during liver transplantation. Data on the resulting pulmonary embolism in liver transplantation showed that although the incidence was not high, the mortality rate was high, and it was particularly common just before or after graft reperfusion [24]. Therefore, diagnosing hypercoagulation state during liver transplantation is an important issue. According to the prospective study by McCrath et al. [25], the increase in MA is an independent predictive factor for post-operative acute myocardial infarction. Another research using ROTEM by Hincker et al. [26] showed that the increase in MCF can predict thrombosis. This emphasizes the need for viscoelastic coagulation test in liver transplantation.

However, some studies reported that there were no differences in bleeding or blood transfusion before and after using this coagulation testing [27]. It can be difficult to definitely conclude whether applying the viscoelastic coagulation test to the blood transfusion strategy during liver transplantation can really improve patient prognosis. However, this test has the clear advantage of reflecting the overall process of coagulation, from clot formation to fibrinolysis, and it can more sensitively and accurately diagnose coagulopathy than CCT during the surgery. For this reason, the application of viscoelastic coagulation test for liver transplantation can reduce bleeding and blood transfusion. Therefore, the Korean guideline for perioperative blood transfusion [28] and the guideline by the American Society of Anesthesiologists [29] consider the blood transfusion strategies using TEG and ROTEM to be important for not only liver transplantation but also perioperative blood management. Moreover, the guidelines for management of severe perioperative bleeding published by the European Society of Anaesthesiology [30] state that CCT is not suitable for surgeries in patients with liver disease and recommend TEG or ROTEM to diagnose perioperative coagulation disorders. Although further study is necessary, this recommendation based on that, viscoelastic coagulation test can improve prognosis by reducing blood transfusion, facilitate targeted management and can identify hyperfibrinolysis.

BLOOD TRANSFUSION STRATEGY USING VISCOELASTIC COAGULATION TEST FOR LIVER TRANSPLANTATION

To date, there is no protocol that applies viscoelastic coagulation test, which was found to be clearly superior as blood transfusion strategies for liver transplantation. A protocol published in 1993 [18] recommended the transfusion of 2–4 units of fresh frozen plasma if R time, which reflects the start of coagulation, is delayed by 15 minutes or more; transfusion of 1 unit/10 kg of platelet if MA, which reflects the clot strength, is thinner than 40 mm; and transfusion of 6–12 units of cryoprecipitate if these blood transfusions do not improve blood coagulation or if the α angle is less than 40 degrees. Findings from research in 2012 showed that using higher TEG threshold for blood transfusion than suggested in this protocol did not increase bleeding [31]. Because there are large variances in the indicators of the coagulation test during liver transplantation, the protocols of the tests are significantly related but not highly consistent [32]. Moreover, since protocols applied in the studies vary, it is difficult to conclude which method was the best. Additionally, the reference values of viscoelastic coagulation test are just average values of healthy subjects. Thus, values that are outside of this range does not necessarily suggest a coagulation disorder. Because values outside the reference range is not directly linked to bleeding, it is difficult to make a protocol based on the test values. In particular, in situations such as liver transplantations in which the state of coagulation changes rapidly, it is not appropriate to perform treatment based only on test results, and it is essential to consider the clinical situation. Research on patients undergoing liver transplantation showed that the predicted cut-off values of risk for bleeding or blood transfusion were within the reference range which further emphasized that treatment decisions based only on test results are inappropriate [14].

Severe fluctuations in the coagulation status appear at each phase of liver transplantation surgery (Fig. 2). Deficiency or functional decline in blood coagulation factor and platelet is typical for the preanhepatic phase. Therefore, thin graphs with extended R and CT and low values of MA and MCF are common. Because it better reflects the balanced coagulation state, stable results can be seen on the viscoelastic test even in patients with severe coagulopathy according to the CCT in the beginning of the surgery. On the other hand, with the progression of the surgery, the worsening of the coagulopathy can be observed in real-time by viscoelastic test. Therefore, while unnecessary plasma transfusion is possible when using only the palliative coagulation test results in the beginning of the surgery, this can be prevented by applying the viscoelastic coagulation test and a treatment can be performed when it is really needed. In the anhepatic phase, the previously mentioned coagulopathy worsens and tPA is not eliminated, and thus, hyperfibrinolysis can occur. Thus, LI30, LI60, LY30, and LY60 increase or worsen. Here, an appropriate administration of an anti-fibrinolysis medication (tranexamic acid) can prevent the worsening of bleeding. In particular, all previously discussed coagulation disorders worsen at the end of the anhepatic phase and immediately after reperfusion, due to the increase in tPA and PAI-1, decrease in alpha-2-antiplasmin, and the influx of heparin-like substances in the donated liver [33-35]. Although coagulopathy is observed in combination, referencing the changes in each value and the results of fibrinogen and platelet functions from the viscoelastic test can be of much help in assessing the most urgently needed treatment. In the neohepatic phase, the coagulopathy can improve due to the functions of the transplanted liver, and this is reflected in the viscoelastic coagulation test results. If the coagulopathy appears unchanged or worsened according to the viscoelastic coagulation test in this phase, the possibility of excessive bleeding must be considered even if this is a generally stable period; in addition, the possibility that the transplanted liver is dysfunctional must be considered as well.

Fig. 2.

Examples of thromboelastography during liver transplantation. (A) Preanhepatic phase. (B) Anhepatic phase. (C) Five minutes after reperfusion. (D) Neohepatic phase. R: reaction time, MA: maximum amplitude.

CONCLUSION

Viscoelastic coagulation test is a point-of-care testing that can show not only the beginning of the coagulation but also the clot strength and stability. It enables faster judgment and treatment than CCT. As its utility has already been shown in clinical situations with coagulation disorders and severe bleeding such as trauma using both clinical and academic studies, this method can be used effectively for liver transplantation which has a high risk of bleeding. Through further research, it is anticipated that this test can be used as one of the standardized patient blood management strategies for liver transplantation.