Literature DB >> 33326107

Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma.

Susana Pascoal1, Sarah Grissenberger1, Eva Scheuringer1, Rita Fior2, Miguel Godinho Ferreira3, Martin Distel4.   

Abstract

Tumor models allowing for the in vivo investigation of molecular mechanisms driving tumor progression and metastasis are important to develop novel strategies for cancer treatment. Unfortunately, for Ewing sarcoma no adequate genetic animal models are currently available. Mouse xenograft models are the state of the art to model Ewing sarcoma in vivo. Here, we describe an alternative Ewing sarcoma xenograft model in embryonic and larval zebrafish. This xenograft model offers live imaging and easy compound testing opportunities hereby complementing mouse xenograft models. In this chapter, we provide a detailed protocol how to xenograft Ewing sarcoma cells (shSK-E17T) into 2-day-old zebrafish and how xenografted zebrafish can be imaged and analyzed over consecutive days to study tumor proliferation.

Entities:  

Keywords:  Ewing sarcoma; Live imaging; Metastasis; Tumor model; Xenotransplantation; Zebrafish

Mesh：

Substances：

Year:  2021        PMID： 33326107     DOI： 10.1007/978-1-0716-1020-6_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  The fate of human malignant melanoma cells transplanted into zebrafish embryos: assessment of migration and cell division in the absence of tumor formation.

Authors:  Lisa M J Lee; Elisabeth A Seftor; Gregory Bonde; Robert A Cornell; Mary J C Hendrix
Journal:  Dev Dyn       Date:  2005-08       Impact factor: 3.780

2.  In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.

Authors:  Myron S Ignatius; Eleanor Chen; Natalie M Elpek; Adam Z Fuller; Inês M Tenente; Ryan Clagg; Sali Liu; Jessica S Blackburn; Corinne M Linardic; Andrew E Rosenberg; Petur G Nielsen; Thorsten R Mempel; David M Langenau
Journal:  Cancer Cell       Date:  2012-05-15       Impact factor: 31.743

3.  Single-cell functional and chemosensitive profiling of combinatorial colorectal therapy in zebrafish xenografts.

Authors:  Rita Fior; Vanda Póvoa; Raquel V Mendes; Tânia Carvalho; António Gomes; Nuno Figueiredo; Miguel Godinho Ferreira
Journal:  Proc Natl Acad Sci U S A       Date:  2017-08-23       Impact factor: 11.205

4.  15 years of zebrafish chemical screening.

Authors:  Andrew J Rennekamp; Randall T Peterson
Journal:  Curr Opin Chem Biol       Date:  2014-11-15       Impact factor: 8.822

5.  High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia.

Authors:  Alexandra C H Smith; Aubrey R Raimondi; Chris D Salthouse; Myron S Ignatius; Jessica S Blackburn; Igor V Mizgirev; Narie Y Storer; Jill L O de Jong; Aye T Chen; Yi Zhou; Sergei Revskoy; Leonard I Zon; David M Langenau
Journal:  Blood       Date:  2010-01-07       Impact factor: 22.113

Review 6.  Zebrafish xenografts as a tool for in vivo studies on human cancer.

Authors:  Martina Konantz; Tugce B Balci; Udo F Hartwig; Graham Dellaire; Maya C André; Jason N Berman; Claudia Lengerke
Journal:  Ann N Y Acad Sci       Date:  2012-08       Impact factor: 5.691

7.  The zebrafish/tumor xenograft angiogenesis assay.

Authors:  Stefania Nicoli; Marco Presta
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

Review 8.  Zebrafish: a new companion for translational research in oncology.

Authors:  Jorge Barriuso; Raghavendar Nagaraju; Adam Hurlstone
Journal:  Clin Cancer Res       Date:  2015-01-08       Impact factor: 12.531

Review 9.  Hooking the big one: the potential of zebrafish xenotransplantation to reform cancer drug screening in the genomic era.

Authors:  Chansey J Veinotte; Graham Dellaire; Jason N Berman
Journal:  Dis Model Mech       Date:  2014-07       Impact factor: 5.758

10.  Combined experience of six independent laboratories attempting to create an Ewing sarcoma mouse model.

Authors:  Tsion Zewdu Minas; Didier Surdez; Tahereh Javaheri; Miwa Tanaka; Michelle Howarth; Hong-Jun Kang; Jenny Han; Zhi-Yan Han; Barbara Sax; Barbara E Kream; Sung-Hyeok Hong; Haydar Çelik; Franck Tirode; Jan Tuckermann; Jeffrey A Toretsky; Lukas Kenner; Heinrich Kovar; Sean Lee; E Alejandro Sweet-Cordero; Takuro Nakamura; Richard Moriggl; Olivier Delattre; Aykut Üren
Journal:  Oncotarget       Date:  2017-05-23
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.