Literature DB >> 33321074

Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.

Giuliano Giuseppe Stirparo1, Austin Smith2, Ge Guo3.   

Abstract

Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Entities:  

Keywords:  SNP; TP53; cancer-related mutations; genetic integrity; naive pluripotency; pluripotent stem cell; sequencing informatics; single nucleotide polymorphism

Mesh：

Year:  2020        PMID： 33321074      PMCID： PMC7837212          DOI： 10.1016/j.stem.2020.11.014

Source DB:  PubMed          Journal:  Cell Stem Cell        ISSN： 1875-9777            Impact factor:   25.269


  18 in total

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2.  Retraction Notice to: Cancer-Related Mutations Identified in Primed and Naive Human Pluripotent Stem Cells.

Authors:  Yishai Avior; Kevin Eggan; Nissim Benvenisty
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3.  Cancer-Related Mutations Identified in Primed Human Pluripotent Stem Cells.

Authors:  Yishai Avior; Elyad Lezmi; Kevin Eggan; Nissim Benvenisty
Journal:  Cell Stem Cell       Date:  2020-12-14       Impact factor: 24.633

4.  Recent insights into the naïve state of human pluripotency and its applications.

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Journal:  Exp Cell Res       Date:  2019-10-01       Impact factor: 3.905

5.  Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.

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6.  Integrative genomics viewer.

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7.  Human Naive Pluripotent Stem Cells Model X Chromosome Dampening and X Inactivation.

Authors:  Anna Sahakyan; Rachel Kim; Constantinos Chronis; Shan Sabri; Giancarlo Bonora; Thorold W Theunissen; Edward Kuoy; Justin Langerman; Amander T Clark; Rudolf Jaenisch; Kathrin Plath
Journal:  Cell Stem Cell       Date:  2016-12-15       Impact factor: 25.269

8.  Resetting transcription factor control circuitry toward ground-state pluripotency in human.

Authors:  Yasuhiro Takashima; Ge Guo; Remco Loos; Jennifer Nichols; Gabriella Ficz; Felix Krueger; David Oxley; Fatima Santos; James Clarke; William Mansfield; Wolf Reik; Paul Bertone; Austin Smith
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9.  Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass.

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10.  XenofilteR: computational deconvolution of mouse and human reads in tumor xenograft sequence data.

Authors:  Roelof J C Kluin; Kristel Kemper; Thomas Kuilman; Julian R de Ruiter; Vivek Iyer; Josep V Forment; Paulien Cornelissen-Steijger; Iris de Rink; Petra Ter Brugge; Ji-Ying Song; Sjoerd Klarenbeek; Ultan McDermott; Jos Jonkers; Arno Velds; David J Adams; Daniel S Peeper; Oscar Krijgsman
Journal:  BMC Bioinformatics       Date:  2018-10-04       Impact factor: 3.169
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  6 in total

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Authors:  Ellen L Larson; Dong Jin Joo; Erek D Nelson; Bruce P Amiot; Rajagopal N Aravalli; Scott L Nyberg
Journal:  Stem Cell Reports       Date:  2021-10-21       Impact factor: 7.765

2.  Large-scale analysis of imprinting in naive human pluripotent stem cells reveals recurrent aberrations and a potential link to FGF signaling.

Authors:  Gal Keshet; Nissim Benvenisty
Journal:  Stem Cell Reports       Date:  2021-09-30       Impact factor: 7.765

Review 3.  The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells.

Authors:  Alexander Keller; Claudia Spits
Journal:  Cells       Date:  2021-11-19       Impact factor: 6.600

4.  Assessing BRCA1 activity in DNA damage repair using human induced pluripotent stem cells as an approach to assist classification of BRCA1 variants of uncertain significance.

Authors:  Meryem Ozgencil; Julian Barwell; Marc Tischkowitz; Louise Izatt; Ian Kesterton; Michael Simpson; Paul Sharpe; Paulo de Sepulveda; Edwige Voisset; Ellen Solomon
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5.  The Tumorigenic Potential of Human Pluripotent Stem Cells.

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Journal:  Stem Cells Transl Med       Date:  2022-08-23       Impact factor: 7.655

6.  Influence of feeder cells on transcriptomic analysis of pluripotent stem cells.

Authors:  Haifeng Wan; Rui Fu; Man Tong; Yukai Wang; Libin Wang; Siqi Wang; Ying Zhang; Wei Li; Xiu-Jie Wang; Guihai Feng
Journal:  Cell Prolif       Date:  2022-01-21       Impact factor: 6.831
  6 in total

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