| Literature DB >> 33278361 |
Romain Forey1, Antoine Barthe1, Mireille Tittel-Elmer2, Maxime Wery3, Marie-Bénédicte Barrault4, Cécile Ducrot5, Andrew Seeber6, Nils Krietenstein7, Ugo Szachnowski3, Magdalena Skrzypczak8, Krzysztof Ginalski8, Maga Rowicka9, Jennifer A Cobb2, Oliver J Rando7, Julie Soutourina4, Michel Werner10, Karine Dubrana5, Susan M Gasser11, Antonin Morillon3, Philippe Pasero1, Armelle Lengronne12, Jérôme Poli13.
Abstract
Mre11-Rad50-Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin with the transcriptional co-regulator Mediator. MRX restricts transcription of coding and noncoding DNA by a mechanism that does not require the nuclease activity of Mre11. MRX is required to tether transcriptionally active loci to the nuclear pore complex (NPC), and it also promotes large-scale gene-NPC interactions. Moreover, MRX-mediated chromatin anchoring to the NPC contributes to chromosome folding and helps to control gene expression. Together, these findings indicate that MRX has a role in transcription and chromosome organization that is distinct from its known function in DNA repair.Entities:
Keywords: MRX/N; Mediator; SMC complexes; chromosomal interaction domains; chromosome folding; chromosome organization; coding and non-coding transcription control; nuclear pore
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Year: 2020 PMID： 33278361 PMCID： PMC8112817 DOI： 10.1016/j.molcel.2020.11.010
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970