Literature DB >> 34027483

3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei.

Mégane Da Mota1, Julien Cau1, Julio Mateos-Langerak1, Armelle Lengronne1, Philippe Pasero1, Jérôme Poli1.   

Abstract

This protocol describes how to culture, image, and determine the nuclear position of a fluorescently tagged DNA locus in the 3D nucleoplasm of fixed Saccharomyces cerevisiae cells. Here, we propose a manual scoring method based on widefield images and an automated method based on 3D-SIM images. Yeast culture conditions have to be followed meticulously to get the best biological response in a given environment. For complete details on the use and execution of this protocol, please refer to Forey et al. (2020).
© 2021 The Author(s).

Entities:  

Keywords:  Cell culture; Gene Expression; Genetics; Microscopy; Model Organisms; Single Cell

Mesh：

Substances：

Year:  2021        PMID： 34027483      PMCID： PMC8122114          DOI： 10.1016/j.xpro.2021.100525

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  17 in total

Review 1.  Three-dimensional imaging by deconvolution microscopy.

Authors:  J G McNally; T Karpova; J Cooper; J A Conchello
Journal:  Methods       Date:  1999-11       Impact factor: 3.608

2.  Methods for visualizing chromatin dynamics in living yeast.

Authors:  Florence Hediger; Angela Taddei; Frank R Neumann; Susan M Gasser
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

3.  Nuclear pore association confers optimal expression levels for an inducible yeast gene.

Authors:  Angela Taddei; Griet Van Houwe; Florence Hediger; Veronique Kalck; Fabien Cubizolles; Heiko Schober; Susan M Gasser
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4.  Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.

Authors:  Mats G L Gustafsson; Lin Shao; Peter M Carlton; C J Rachel Wang; Inna N Golubovskaya; W Zacheus Cande; David A Agard; John W Sedat
Journal:  Biophys J       Date:  2008-03-07       Impact factor: 4.033

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Authors:  Patrice Mascalchi; Fabrice P Cordelières
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Review 6.  Strategic and practical guidelines for successful structured illumination microscopy.

Authors:  Justin Demmerle; Cassandravictoria Innocent; Alison J North; Graeme Ball; Marcel Müller; Ezequiel Miron; Atsushi Matsuda; Ian M Dobbie; Yolanda Markaki; Lothar Schermelleh
Journal:  Nat Protoc       Date:  2017-04-13       Impact factor: 13.491

7.  SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope.

Authors:  Ghislain G Cabal; Auguste Genovesio; Susana Rodriguez-Navarro; Christophe Zimmer; Olivier Gadal; Annick Lesne; Henri Buc; Frank Feuerbach-Fournier; Jean-Christophe Olivo-Marin; Eduard C Hurt; Ulf Nehrbass
Journal:  Nature       Date:  2006-06-08       Impact factor: 49.962

8.  Nup-PI: the nucleopore-promoter interaction of genes in yeast.

Authors:  Manfred Schmid; Ghislaine Arib; Caroline Laemmli; Junichi Nishikawa; Thérèse Durussel; Ulrich K Laemmli
Journal:  Mol Cell       Date:  2006-02-03       Impact factor: 17.970

9.  Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells.

Authors:  N Belgareh; V Doye
Journal:  J Cell Biol       Date:  1997-02-24       Impact factor: 10.539

10.  Global reorganization of budding yeast chromosome conformation in different physiological conditions.

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