Amira Zarrouk1, Souha Hammouda1, Imen Ghzaiel1, Sonia Hammami1, Wided Khamlaoui1, Samia Hadj Ahmed1, Gérard Lizard2, Mohamed Hammami1.
Abstract
OBJECTIVE: Oxidative stress is the main feature of several diseases including Alzheimer's disease (AD). The involvement of oxysterols derivates has been recently reported. In this study, the implication of oxidative stress in cholesterol impairment in AD patients will be evaluated.
METHODS: A case-control study was conducted on 56 AD patients and 97 controls. Levels of oxidative biomarkers, including lipid peroxidation products and antioxidant enzyme activities were measured with spectrophotometric methods on red blood cells (RBCs) and plasma. Cholesterol precursors and oxysterols (7KC, 7α-OHC, 7β-OHC, 24S-OH, 27-OHC, and 25-OHC) in plasma were quantified by gas chromatography coupled with mass spectrometry. In RBCs and plasma of AD patients, a significant decrease of glutathione peroxidase (GPx) activity was detected associated with raised levels of malondialdehyde (MDA). A decreased level of lanosterol and an accumulation of 7β-OHC, 24S-OHC, 27-OHC, and 25-OHC that were higher in plasma of AD patients, compared to controls, were also observed in AD patients.
CONCLUSION: Mini-Mental State Examination (MMSE) score was correlated with MDA and conjugated dienes (CD) levels in plasma. Besides, the MDA level in RBCs was correlated with 7βhydroxycholesterol (7β-OHC). Binary logistic regression revealed an association between GPx activity and AD (OR=0.895, 95%CI: 0.848-0.945; P<0.001). Our data consolidate the relationship between the rupture of redox homeostasis and lipid and cholesterol oxidation in AD. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
OBJECTIVE: Oxidative stress is the main feature of several diseases including Alzheimer's disease (AD). The involvement of oxysterols derivates has been recently reported. In this study, the implication of oxidative stress in cholesterol impairment in AD patients will be evaluated.
METHODS: A case-control study was conducted on 56 AD patients and 97 controls. Levels of oxidative biomarkers, including lipid peroxidation products and antioxidant enzyme activities were measured with spectrophotometric methods on red blood cells (RBCs) and plasma. Cholesterol precursors and oxysterols (7KC, 7α-OHC, 7β-OHC, 24S-OH, 27-OHC, and 25-OHC) in plasma were quantified by gas chromatography coupled with mass spectrometry. In RBCs and plasma of AD patients, a significant decrease of glutathione peroxidase (GPx) activity was detected associated with raised levels of malondialdehyde (MDA). A decreased level of lanosterol and an accumulation of 7β-OHC, 24S-OHC, 27-OHC, and 25-OHC that were higher in plasma of AD patients, compared to controls, were also observed in AD patients.
CONCLUSION: Mini-Mental State Examination (MMSE) score was correlated with MDA and conjugated dienes (CD) levels in plasma. Besides, the MDA level in RBCs was correlated with 7βhydroxycholesterol (7β-OHC). Binary logistic regression revealed an association between GPx activity and AD (OR=0.895, 95%CI: 0.848-0.945; P<0.001). Our data consolidate the relationship between the rupture of redox homeostasis and lipid and cholesterol oxidation in AD. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
Entities:
Keywords:
Alzheimer's disease; antioxydant enzymes; cholesterol.; lipid peroxidation; oxidative stress; oxysterols
Year: 2020
PMID： 33272182 DOI： 10.2174/1567205017666201203123046
Source DB: PubMed Journal: Curr Alzheimer Res ISSN： 1567-2050 Impact factor: 3.498