Shanqiang Qu1,2, Zhixin Chen3, Bin Liu4, Jin Liu5, Huafu Wang6. 1. Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. 2. Department of Neurosurgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. 3. Department of Emergency Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China. 4. Department of Orthopedic, Lishui People's Hospital (The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China. 5. Department of Neurosurgery, Lishui People's Hospital (The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China. 6. Department of Clinical Pharmacy, Lishui People's Hospital (The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China.
Abstract
BACKGROUND: Although aberrant expression of N6-methyladenine (m6 A) methylation-related genes contribute to tumorigenesis in many solid tumors, the prognostic value of the m6 A-related genes and their correlation with clinicopathological features in gliomas need advanced study. METHODS: The clinical and sequencing data of 288 patients with glioma were extracted from Chinese Glioma Genome Atlas database. By univariate and multivariable Cox regression analysis, the m6 A-related prognostic genes were identified, and their correlation with clinicopathological features was further analysis. A nomogram was constructed by R software and the performance of it was assessed by calibration and time-dependent receiver operating characteristic curve. RESULTS: Nine m6 A-related genes were identified as independent prognostic factors, which were mostly enriched in RNA splicing, regulation of immune response and vesicle-mediated transport. By expression value and regression coefficient of these genes, we constructed risk score of each patient, which was highly associated with clinicopathological features. Kaplan-Meier curve showed that the prognosis of patients with high-risk scores was significantly worse than that with low-risk scores (HR = 4.30, 95% CI = 3.16-5.85, p < 0.0001). A nomogram was constructed based on the nine m6 A-related genes signature and clinicopathological features with well-fitted calibration curves (c-index = 0.82), showing high specificity and sensitivity (area under the curve for 1-, 3-, and 5-years survival probability = 0.874, 0.918, and 0.934). CONCLUSIONS: A nine m6 A-related genes signature was identified in gliomas. The m6 A-related risk score is a novel prognostic factor for patients with glioma, and is associated with clinicopathological features. Moreover, the nomogram based on the nine m6 A-related genes signature and clinicopathological features had good efficacy in predicting the survival probability.
BACKGROUND: Although aberrant expression of N6-methyladenine (m6 A) methylation-related genes contribute to tumorigenesis in many solid tumors, the prognostic value of the m6 A-related genes and their correlation with clinicopathological features in gliomas need advanced study. METHODS: The clinical and sequencing data of 288 patients with glioma were extracted from Chinese Glioma Genome Atlas database. By univariate and multivariable Cox regression analysis, the m6 A-related prognostic genes were identified, and their correlation with clinicopathological features was further analysis. A nomogram was constructed by R software and the performance of it was assessed by calibration and time-dependent receiver operating characteristic curve. RESULTS: Nine m6 A-related genes were identified as independent prognostic factors, which were mostly enriched in RNA splicing, regulation of immune response and vesicle-mediated transport. By expression value and regression coefficient of these genes, we constructed risk score of each patient, which was highly associated with clinicopathological features. Kaplan-Meier curve showed that the prognosis of patients with high-risk scores was significantly worse than that with low-risk scores (HR = 4.30, 95% CI = 3.16-5.85, p < 0.0001). A nomogram was constructed based on the nine m6 A-related genes signature and clinicopathological features with well-fitted calibration curves (c-index = 0.82), showing high specificity and sensitivity (area under the curve for 1-, 3-, and 5-years survival probability = 0.874, 0.918, and 0.934). CONCLUSIONS: A nine m6 A-related genes signature was identified in gliomas. The m6 A-related risk score is a novel prognostic factor for patients with glioma, and is associated with clinicopathological features. Moreover, the nomogram based on the nine m6 A-related genes signature and clinicopathological features had good efficacy in predicting the survival probability.
Authors: Rafay Ahmed; Matthew J Oborski; Misun Hwang; Frank S Lieberman; James M Mountz Journal: Cancer Manag Res Date: 2014-03-24 Impact factor: 3.989
Authors: Shao-Hua Ren; Ya-Fei Qin; Hong Qin; Hong-da Wang; Guang-Ming Li; Yang-Lin Zhu; Cheng-Lu Sun; Bo Shao; Jing-Yi Zhang; Jing-Peng Hao; Hao Wang Journal: Int J Gen Med Date: 2022-03-30