BACKGROUND: Globally, noroviruses are recognized as an important cause of acute gastroenteritis (AGE), but data from low and middle-income countries are limited. AIMS: To examine the epidemiology and strain diversity of norovirus infections among children hospitalized for AGE in Bangladesh. METHODS: We implemented active surveillance of children <5 years of age hospitalized with AGE at 8 geographically dispersed tertiary care hospitals in Bangladesh from July 2012 to June 2016. We tested random samples of AGE cases stratified by site and age group for norovirus by real-time RT-PCR. Noro-positive specimens were genotyped. Coinfection with rotavirus was assessed based on prior EIA testing. RESULTS: We enrolled 5622 total AGE cases, of which 1008 were tested for norovirus. Total of 137 (14%) AGE cases tested positive for norovirus (range, 11%-17% by site). Most (94%) norovirus-associated hospitalizations were among children less than 2 years of age. Norovirus was detected year-round, with higher detection from March to June (20%-38%) and November to January (9%-18%). Genogroup II (GII) noroviruses were detected in 96% of cases, and the most frequent genotypes were GII.4 Sydney [P4 New Orleans] (33%), GII.3 [P16] (20%), and GII.4 Sydney [P16] (11%). The proportion of norovirus-positive specimens was significantly greater among rotavirus-negative AGE patients compared with rotavirus-positive AGE patients (27% vs. 5%, P < 0.001). As measured by the Vesikari severity score, a similar proportion of norovirus and rotavirus positive AGE patients were considered severe (68% vs. 70%, P = 0.86). CONCLUSIONS: Norovirus is an important cause of AGE hospitalization in Bangladeshi children with most infections caused by GII viruses.
BACKGROUND: Globally, noroviruses are recognized as an important cause of acute gastroenteritis (AGE), but data from low and middle-income countries are limited. AIMS: To examine the epidemiology and strain diversity of norovirus infections among children hospitalized for AGE in Bangladesh. METHODS: We implemented active surveillance of children <5 years of age hospitalized with AGE at 8 geographically dispersed tertiary care hospitals in Bangladesh from July 2012 to June 2016. We tested random samples of AGE cases stratified by site and age group for norovirus by real-time RT-PCR. Noro-positive specimens were genotyped. Coinfection with rotavirus was assessed based on prior EIA testing. RESULTS: We enrolled 5622 total AGE cases, of which 1008 were tested for norovirus. Total of 137 (14%) AGE cases tested positive for norovirus (range, 11%-17% by site). Most (94%) norovirus-associated hospitalizations were among children less than 2 years of age. Norovirus was detected year-round, with higher detection from March to June (20%-38%) and November to January (9%-18%). Genogroup II (GII) noroviruses were detected in 96% of cases, and the most frequent genotypes were GII.4 Sydney [P4 New Orleans] (33%), GII.3 [P16] (20%), and GII.4 Sydney [P16] (11%). The proportion of norovirus-positive specimens was significantly greater among rotavirus-negative AGE patients compared with rotavirus-positive AGE patients (27% vs. 5%, P < 0.001). As measured by the Vesikari severity score, a similar proportion of norovirus and rotavirus positive AGE patients were considered severe (68% vs. 70%, P = 0.86). CONCLUSIONS: Norovirus is an important cause of AGE hospitalization in Bangladeshi children with most infections caused by GII viruses.
Authors: Jennifer L Cannon; Leslie Barclay; Nikail R Collins; Mary E Wikswo; Christina J Castro; Laura Cristal Magaña; Nicole Gregoricus; Rachel L Marine; Preeti Chhabra; Jan Vinjé Journal: J Clin Microbiol Date: 2017-05-10 Impact factor: 5.948
Authors: Preeti Chhabra; Miranda de Graaf; Gabriel I Parra; Martin Chi-Wai Chan; Kim Green; Vito Martella; Qiuhong Wang; Peter A White; Kazuhiko Katayama; Harry Vennema; Marion P G Koopmans; Jan Vinjé Journal: J Gen Virol Date: 2019-10 Impact factor: 3.891
Authors: M Rahman; Z Hassan; Z Nahar; A S G Faruque; M Van Ranst; S R Rahman; T Azim Journal: Eur J Clin Microbiol Infect Dis Date: 2010-05-14 Impact factor: 3.267
Authors: Daniel C Payne; Jan Vinjé; Peter G Szilagyi; Kathryn M Edwards; Mary Allen Staat; Geoffrey A Weinberg; Caroline B Hall; James Chappell; David I Bernstein; Aaron T Curns; Mary Wikswo; S Hannah Shirley; Aron J Hall; Benjamin Lopman; Umesh D Parashar Journal: N Engl J Med Date: 2013-03-21 Impact factor: 91.245
Authors: J Joukje Siebenga; Harry Vennema; Bernadet Renckens; Erwin de Bruin; Bas van der Veer; Roland J Siezen; Marion Koopmans Journal: J Virol Date: 2007-07-03 Impact factor: 5.103
Authors: Stephanie Chow Garbern; Eric J Nelson; Sabiha Nasrin; Adama Mamby Keita; Ben J Brintz; Monique Gainey; Henry Badji; Dilruba Nasrin; Joel Howard; Mami Taniuchi; James A Platts-Mills; Karen L Kotloff; Rashidul Haque; Adam C Levine; Samba O Sow; Nur Haque Alam; Daniel T Leung Journal: Elife Date: 2022-02-09 Impact factor: 8.140
Authors: Sultana Yeasmin; S M Tafsir Hasan; Mohammod Jobayer Chisti; Md Alfazal Khan; A S G Faruque; Tahmeed Ahmed Journal: PLoS One Date: 2022-08-26 Impact factor: 3.752