Aline Satie Takamiya1, Douglas Roberto Monteiro2, Luiz Fernando Gorup3, Ebele Adaobi Silva4, Emerson Rodriques de Camargo5, João Eduardo Gomes-Filho6, Sandra Helena Penha de Oliveira7, Debora Barros Barbosa8. 1. Department of Diagnosis and Surgery, Araçatuba Dental School, Univ Estadual Paulista - UNESP, Araçatuba, São Paulo, Brazil. Electronic address: aline.takamiya@unesp.br. 2. Department of Pediatric Dentistry and Public Health Dental, Araçatuba Dental School, Univ Estadual Paulista - UNESP, Araçatuba, São Paulo, Brazil. 3. LIEC-Department of Chemistry, Federal University of São Carlos - UFSCar, São Carlos, São Paulo, Brazil. 4. Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, São Paulo State University, Ribeirão Preto, São Paulo, Brazil. 5. LIEC-Department of Chemistry, Federal University of São Carlos - UFSCar, São Carlos, São Paulo, Brazil. Electronic address: camargo@ufscar.br. 6. Department of Endodontics, Araçatuba Dental School, Univ Estadual Paulista - UNESP, Araçatuba, São Paulo, Brazil. Electronic address: joao@foa.unesp.br. 7. Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista - UNESP, Araçatuba, São Paulo, Brazil. Electronic address: sandra.hp.oliveira@unesp.br. 8. Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista - UNESP, Araçatuba, São Paulo, Brazil.
Abstract
BACKGROUND: Although silver nanoparticles (SNP) have proven antimicrobial activity against different types of microorganisms, the effect of SNP incorporation into acrylic resin to control Candida albicans biofilm formation aiming at the prevention of Candida-associated denture stomatitis has not yet been fully elucidated. OBJECTIVES: This study aimed to evaluate the antimicrobial effect of an acrylic resin containing SNP on C. albicans biofilm growth, the flexural strength of this material and tissue reaction in the subcutaneous connective tissue of rats to SNP. METHOD: SNP were synthesized through silver nitrate reduction by sodium citrate. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to verify the size and colloidal stability. SNP were added to acrylic resin monomer (Lucitone 550) at 0.05, 0.5 and 5 vol%. The antimicrobial effect against C. albicans (ATCC 10231) was investigated by the enumeration of colony-forming units (CFUs) and SEM. The three-point bending test was performed to analyze the flexural strength. Tissue reaction was evaluated after 7 and 60 days of implantation in the connective tissue of Wistar rats. RESULTS: Spherical particles of 5 and 10 nm were obtained. SNP at 0.05 and 0.5% incorporated into acrylic resin was effective in reducing C. albicans biofilm growth (p < .001). SEM revealed that the material was able to disrupt C. albicans biofilm formation and did not reduce the flexural strength compared to control (p > .05). The inflammatory response observed 60 days after implantation SNP in the subcutaneous tissue was similar to control. CONCLUSION: It was concluded that SNP addition at 0.05 and 0.5% into acrylic resin exhibited antimicrobial effects against C. albicans biofilm, did not interfere in the flexural strength and may be considered biocompatible.
BACKGROUND: Although silver nanoparticles (SNP) have proven antimicrobial activity against different types of microorganisms, the effect of SNP incorporation into acrylic resin to control Candida albicans biofilm formation aiming at the prevention of Candida-associated denture stomatitis has not yet been fully elucidated. OBJECTIVES: This study aimed to evaluate the antimicrobial effect of an acrylic resin containing SNP on C. albicans biofilm growth, the flexural strength of this material and tissue reaction in the subcutaneous connective tissue of rats to SNP. METHOD: SNP were synthesized through silver nitrate reduction by sodium citrate. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to verify the size and colloidal stability. SNP were added to acrylic resin monomer (Lucitone 550) at 0.05, 0.5 and 5 vol%. The antimicrobial effect against C. albicans (ATCC 10231) was investigated by the enumeration of colony-forming units (CFUs) and SEM. The three-point bending test was performed to analyze the flexural strength. Tissue reaction was evaluated after 7 and 60 days of implantation in the connective tissue of Wistar rats. RESULTS: Spherical particles of 5 and 10 nm were obtained. SNP at 0.05 and 0.5% incorporated into acrylic resin was effective in reducing C. albicans biofilm growth (p < .001). SEM revealed that the material was able to disrupt C. albicans biofilm formation and did not reduce the flexural strength compared to control (p > .05). The inflammatory response observed 60 days after implantation SNP in the subcutaneous tissue was similar to control. CONCLUSION: It was concluded that SNP addition at 0.05 and 0.5% into acrylic resin exhibited antimicrobial effects against C. albicans biofilm, did not interfere in the flexural strength and may be considered biocompatible.
Authors: Dan Cristian Gheorghe; Adelina-Gabriela Niculescu; Alexandra Cătălina Bîrcă; Alexandru Mihai Grumezescu Journal: Pharmaceutics Date: 2021-05-27 Impact factor: 6.321