Shen Gu1,2,3,4, Wei He5, Minghao Yan2,3, Jian He6, Qun Zhou6, Xiaopeng Yan1, Xiao Fu1,2,3, Jun Chen7, Xiaodong Han2,3, Yudong Qiu1. 1. Department of Hepatopancreatobiliary Surgery, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China. 2. Immunology and Reproduction Biology Laboratory & State Key Laboratory of Analytical Chemistry for Life Science, Medical School, Nanjing University, Nanjing, China. 3. Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, China. 4. Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Hangzhou First People's Hospital, Hangzhou, China. 5. Department of Neurosurgery, Affiliated Aoyang Hospital of Jiangsu University, Zhangjiagang, China. 6. Department of Radiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. 7. Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
Abstract
BACKGROUND & AIMS: Intrahepatic cholangiocarcinoma (ICC) has over the last 10 years become the focus of increasing concern largely due to its rising incidence and high mortality rates worldwide. Microcystin-leucine-arginine (MC-LR) has been reported to be carcinogenic, but there are no data on the linkage between MC-LR and ICC. This study aimed to explore whether the content levels of MC-LR in the tumour tissues of ICC patients be associated with the prognosis and if so, to characterize the mechanism in ICC cells. METHODS: We conducted a retrospective study to evaluate the prognostic value of MC-LR in ICC after resection. All patients were divided into two groups according to the content of MC-LR in tumour via immunohistochemistry: low-MC-LR group (n = 28) and high-MC-LR group (n = 30). RESULTS: Multivariate analysis showed high-MC-LR level was the prognostic factor for OS and RFS after hepatectomy (P = .011 and .044). We demonstrated that MC-LR could promote the survival of human ICC cell lines and SET was identified as an important mRNA in the progression via RNA array. CONCLUSIONS: We provide evidence that MC-LR was an independent prognostic factor for ICC in humans by modulating the expression of SET in human ICC cells.
BACKGROUND & AIMS:Intrahepatic cholangiocarcinoma (ICC) has over the last 10 years become the focus of increasing concern largely due to its rising incidence and high mortality rates worldwide. Microcystin-leucine-arginine (MC-LR) has been reported to be carcinogenic, but there are no data on the linkage between MC-LR and ICC. This study aimed to explore whether the content levels of MC-LR in the tumour tissues of ICC patients be associated with the prognosis and if so, to characterize the mechanism in ICC cells. METHODS: We conducted a retrospective study to evaluate the prognostic value of MC-LR in ICC after resection. All patients were divided into two groups according to the content of MC-LR in tumour via immunohistochemistry: low-MC-LR group (n = 28) and high-MC-LR group (n = 30). RESULTS: Multivariate analysis showed high-MC-LR level was the prognostic factor for OS and RFS after hepatectomy (P = .011 and .044). We demonstrated that MC-LR could promote the survival of human ICC cell lines and SET was identified as an important mRNA in the progression via RNA array. CONCLUSIONS: We provide evidence that MC-LR was an independent prognostic factor for ICC in humans by modulating the expression of SET in human ICC cells.
Authors: Otto Kauko; Caitlin M O'Connor; Evgeny Kulesskiy; Jaya Sangodkar; Anna Aakula; Sudeh Izadmehr; Laxman Yetukuri; Bhagwan Yadav; Artur Padzik; Teemu Daniel Laajala; Pekka Haapaniemi; Majid Momeny; Taru Varila; Michael Ohlmeyer; Tero Aittokallio; Krister Wennerberg; Goutham Narla; Jukka Westermarck Journal: Sci Transl Med Date: 2018-07-18 Impact factor: 17.956
Authors: Sofia S Pereira; Mariana P Monteiro; Madalena M Costa; Jorge Ferreira; Marco G Alves; Pedro F Oliveira; Ivana Jarak; Duarte Pignatelli Journal: J Cell Biochem Date: 2018-09-06 Impact factor: 4.429
Authors: Igor Mrdjen; Mark A Morse; Randall J Ruch; Thomas J Knobloch; Shambhunath Choudhary; Christopher M Weghorst; Jiyoung Lee Journal: Toxins (Basel) Date: 2018-10-28 Impact factor: 4.546