Outcomes of Epiretinal Membrane Removal Utilizing Triamcinolone Acetonide Visualization and Internal Limiting Membrane Forceps.

PURPOSE: To evaluate epiretinal membrane (ERM) removal utilizing internal limiting membrane (ILM) forceps and visualization with triamcinolone acetonide (TA).
METHODS: Retrospective interventional case series of eyes undergoing ERM removal with TA visualization with follow-up of up to five years. Best-corrected visual acuity (BCVA), central macular thickness (CMT), and incidence of complications were reviewed.
RESULTS: A total of 132 eyes were included with 54 and 16 eyes completing 3- and 5-year follow-up, respectively. Mean BCVA and CMT improved significantly at all postoperative evaluations compared to baseline (p<0.001). Pre-operative presence of PVD did not affect outcome measures. No intraoperative complications were reported. Immediate post-operative complications included one case of sterile endophthalmitis and one case of vitreous and perimacular hemorrhage. At one year, complications included progression of cataract in phakic eyes (65.4%), steroid-induced glaucoma (2.2%), retinal tear (0.8%), recurrent ERM (4.5%), and recurrent macular edema (11.3%). No further complications were reported at three- and five-year follow-up.
CONCLUSION: Combination ILM forceps and TA visualization offers an affordable and safe option for ERM removal with comparable or better outcomes than traditional methods of ERM surgery.

Introduction

An epiretinal membrane (ERM), also known as a macular pucker, is a fibrocellular tissue that proliferates on the surface of the internal limiting membrane (ILM). It can be idiopathic or secondary to retinal tear or detachment, trauma, intraocular surgery, ocular inflammatory diseases, or retinal vascular disorders such as diabetic retinopathy or retinal vein occlusion.1 Although many patients with ERMs can be asymptomatic, they can also cause blurred vision and metamorphopsia.1,2

ERM surgery is perhaps the most common vitreoretinal procedure performed. It involves removing the fibrocellular tissue from the retinal surface with or without the ILM.2 In recent years, there is a movement towards using dyes to stain the retinal surface to facilitate the procedure. Trypan blue (Tb), brilliant blue G (BBG), and now less commonly, indocyanine green (ICG) have been utilized for this purpose.3–5 There are concerns about toxicity with some of these agents.6–8

We report the largest case series to date utilizing intravitreal triamcinolone acetonide (TA) for visualization of ERM and membrane peeling with ILM forceps. The aim of this study is to report outcomes including best corrected visual acuity (BCVA), change in central macular thickness (CMT) on optical coherence tomography (OCT), and complications at one, three, and five years following surgery.

Methods

This retrospective interventional case series included all patients who underwent pars plana vitrectomy (PPV) for ERM with TA visualization and membrane peel with ILM forceps between January 2014 and January 2019 by a single physician (R.P.G.). Each patient received a complete ophthalmologic exam including BCVA and spectral domain-optical coherence tomography (Cirrus; Carl Zeiss Meditec AG, Jena, Germany) pre- and post-operatively. Patients were followed at one, three, six, twelve, thirty-six, and sixty months post-operatively. Fluorescein angiography (FA) was performed pre- and post-operatively in most cases.

This study was performed in accordance with the tenants of the Declaration of Helsinki and IRB exempt status was obtained by the Sterling IRB (IRB ID: 0877-RPGallemore). Written and informed consent was obtained from patients for publication of their cases and any accompanying images. Individual health information remained protected, and data confidentiality was maintained throughout the collection of data and writing of this article according to the Health Insurance Portability and Accountability Act (HIPPA) Privacy Rule and Security Rule.

Procedure

A standard 23-gauge three port vitrectomy technique was utilized to peel ERMs and the underlying ILM. After a quarter vitrectomy involving removal of the central vitreous and Weiss ring, and in some cases, creation of a posterior vitreous detachment (PVD), TA (TRIESENCE, Alcon Laboratories Inc., Fort Worth, TX) was applied to the retinal surface using a 25-gauge five eighths inch needle bevel down towards the retinal surface. Enough was used to cover the macula from arcade to arcade, then allowed to sit for moments, and aspirated off the retinal surface, leaving some granules behind. ILM forceps were then utilized to engage the retinal surface and pull back the ERM. TA was then added back, aspirated once again, and the underlying ILM was peeled away from arcade to arcade. Both the ERM and ILM were removed from the eye. Periphery was inspected with scleral depression to look for any new tears or detachments and treated with endolaser when required.

Study Assessments

Patients were examined pre-operatively, postoperatively day one and months one, three, six, and twelve in addition to years 3 and 5. During each visit, patients were examined with a complete ophthalmologic examination including assessment of BCVA and intraocular pressure (IOP). Additional testing including OCT imaging and, when indicated, fluorescein angiography (FA) were performed at follow-up visits. BCVA was assessed with the Snellen eye chart. Average CMT was calculated as the average of the foveal and four perifoveal ETDRS subfields within the 3mm circle displayed on OCT retinal thickness maps. Recurrent ME was defined as development of intraretinal cysts on OCT images or leakage seen on FA.

Statistical Analysis

All data were recorded on Microsoft Excel and statistical analysis was performed using Data Analysis Toolpak software. All snellen visual acuity values were converted to the logarithm of the minimum angle of resolution (logMAR). Patients who completed at least twelve months of follow up were included for analysis of baseline demographics, complications, and change in BCVA and CMT. Changes after surgery were determined as the difference between preoperative values and postoperative values at months one, three, six, and twelve, and years three and five. A paired Student’s t-test was used to compare preoperative to postoperative BCVA and CMT. A p-value of <0.05 was considered statistically significant.

Results

Of 142 identified eyes, 132 (92%) were included for analysis. Mean age was 67.7±11.4 years. Idiopathic ERMs were found in 62 (46.9%) eyes and secondary ERMs in 70 (53.1%) eyes (Table 1). 92% (n=132) completed 1 year follow up, 38% (n=54) completed 3-year follow up, and 11% (n=16) completed 5-year follow up.

Table 1

Baseline Demographics

Age (Years)Mean ± Standard Deviation	67.7±11.4
GenderMale:Female	72:60
Lens StatusPhakic:Pseudophakic:Aphakic	81:51:0
Idiopathic Epiretinal MembraneNumber of Eyes (%)	62 (46.9%)
Secondary Epiretinal MembraneNumber of Eyes (%)	70 (53.1%)
History of Retinal Detachment or Tear	32 (45.7%*)
History of Pars Plana Vitrectomy	2 (2.9%*)
Branch Retinal Vein Occlusion	6 (8.6%*)
Central Retinal Vein Occlusion	2 (2.2%*)
Uveitis	1 (1.4%*)
Nonproliferative Diabetic Retinopathy	14 (20.0%*)
Proliferative Diabetic Retinopathy	3 (4.2%*)
Exudative Macular Degeneration	10 (14.3%*)

Note: *Percentages calculated based on 70 eyes with secondary epiretinal membranes.

BCVA and Average CMT

Mean pre-operative BCVA was 0.579± 0.417 logMAR (Snellen 20/75) and CMT 414.6±69.3 micrometers. At 1-year post-operative BCVA improved by a mean 0.275±0.033 logMAR (p<0.001) and CMT decreased by a mean of 70.5±7.8 micrometers (p<0.001). Similar statistically significant improvements were noted at months 1, 3, 6, 12, 36, and 60 (p<0.05, Figures 1 and 2).

Figure 1

Mean change in best corrected visual acuity (BCVA) compared to preoperative BCVA at months one, three, six, twelve, thirty six, and sixty. Error bars represent the standard error of the mean value (SEM). Statistically significant improvements occurred at each month of follow-up (p<0.05).

Figure 2

Mean change in central macular thickness (CMT) compared to preoperative CMT at months one, three, six, twelve, thirty six, and sixty. Error bars represent the standard error of the mean value (SEM). Statistically significant improvements occurred at each month of follow-up (p<0.05).

Patients without PVD (n=51) had a mean preoperative BCVA of 0.653±0.409 logMAR with CMT of 470.7±96.2 micrometers and patients with PVD (n=81) had mean pre-operative BCVA of 0.470+/0.303logMAR with CMT of 454.9±75.9 micrometers. At one year, patients without PVD had BCVA 0.402±0.364 logMAR with CMT 367.8±50.0 microns and patients with PVD had BCVA 0.165±0.219 logMAR with CMT 387.3±53.8 microns. There was a statistically significant difference in pre-operative BCVA (P<0.001) and BCVA at one year (p≪0.001) between the two groups. There was not a significant difference in change in BCVA and CMT from preoperative to final postoperative visit between eyes with PVD compared to eyes without PVD (Figures 3 and 4).

Figure 3

Change in best-corrected visual acuity from preoperative to final postoperative visit in eyes with and without preoperative posterior vitreous detachments (PVDs). Differences were not statistically (p=0.44).

Figure 4

Change in central macular thickness from preoperative to final postoperative visit in eyes with and without preoperative posterior vitreous detachments (PVDs). Differences were not statistically significant (p=0.20).

Complications

No intraoperative complications were reported. Immediate post-operative complications included 1 case of sterile endophthalmitis presumptively treated with intravitreal antibiotics but vitreous tap was without microorganism growth. Another case developed vitreous and premacular hemorrhage immediately postoperatively in a patient who had forgotten to discontinue aspirin and plavix prior to surgery.

Cataract progression was seen in 53 phakic eyes (65.4%) with 32 (60.0%) undergoing surgery. The majority of these occurred within the first year following surgery. 4.5% (n=6) developed recurrent ERM and 11.3% (n=15) developed recurrent ME post-operatively. Three (2.2%) eyes developed steroid-induced glaucoma which was successfully managed medically in all cases (Table 2).

Table 2

Complications

	Number of Eyes (%)
Cataract Progression or Surgeryin Phakic Eyes (n = 81)	53 (65.4%)
Steroid-Induced Glaucoma	3 (2.2%)
Sterile Endophthalmitis	1 (0.8%)
Vitreous Hemorrhage	1 (0.8%)
Retinal Tear	1 (0.8%)
Recurrent Epiretinal Membrane	6 (4.5%)
Idiopathic	3 (50.0%*)
Proliferative Diabetic Retinopathy	1 (16.7%*)
Exudative Macular Degeneration	2 (33.3%*)
Recurrent Macular Edema	15 (11.3%)
Nonproliferative Diabetic Retinopathy	1 (6.7%†)
Proliferative Diabetic Retinopathy	4 (26.7%†)
Irvine Gass	3 (20.0%†)
s/p YAG Capsulotomy	2 (13.3%†)
Exudative Macular Degeneration	2 (13.3%†)
Recurrent Epiretinal Membrane	2 (13.3%†)
Intraoperative Complications	0 (0%)

Notes: *Percentages calculated based on 6 eyes with recurrent epiretinal membrane. †Percentages calculated based on 15 eyes with recurrent macular edema.

Discussion

We demonstrated that TA can facilitate visualization of the ERM and ILM with outcomes equal to or better than ERM surgery with traditional methods of staining.2,9–14

There was a statistically significant improvement in both BCVA and CMT at all postoperative time points. Although preoperative values are highly varied among prior studies, they all demonstrated similar improvements with one year postoperative vision ranging between 0.180 and 0.690 logMAR (Snellen 20/30 to 20/100) and CMT 268–375µm.9,10,13–15 BCVA at five years was 0.109±0.029 logMAR (Snellen 20/25), which is better than another study reporting outcomes of ERM surgery with ICG visualization (0.449±0.487logMAR; Snellen 20/60).2 Dawson et al demonstrated that surgical intervention on patients with better preoperative BCVA yields a better final BCVA and patients with poorer preoperative BCVA showed a greater change in BCVA following ERM surgery.10 This may account for the greater postoperative BCVA in the 5-year follow-up group as their mean preoperative BCVA was 0.33 logMAR (Snellen 20/40). It is important to note that given the patients' associated macular edema with these patients’ underlying condition, all were maintained on topical anti-inflammatory drops with few on topical steroids, and often in combination with antiglaucoma drops, pre- and post-operatively, which may confound these results in our study cohort.

Despite having similar preoperative CMT values, eyes with PVD were found to have a significantly better preoperative BCVA compared to eyes without PVD (p<0.001). BCVA one year post-operatively in the PVD group was also significantly better than eyes without PVD (p≪0.001), likely due to the better pre-operative BCVA in the eyes with PVD compared to the eyes without PVD. Both groups showed similar trends in improvement in BCVA and CMT postoperatively at one year and final visit, suggesting that creation of a PVD intra-operatively does not affect visual or anatomic outcomes as measured by BCVA and CMT.

Interestingly, no intraoperative retinal tears were noted in our study, which has been reported to be as high as 6% in one study and 8.9% in eyes being operated on with dyes.10,13 These findings are consistent with a multicentered controlled clinical trial that demonstrated eyes undergoing PPV with TA showed a reduced incidence of intraoperative complications including retinal detachment and retinal breaks compared to eyes undergoing traditional PPV without dyes.16 However, it is important to note that the results in our study were obtained from one surgeon and similar surgical techniques in other hands may demonstrate different intraoperative results.

There is also concern for cataract formation, glaucoma, and endophthalmitis with the use of steroids. In our study, cataract progression was the most common finding (65.4% in phakic eyes) with rates comparable to other studies ranging from 28%-89%.10,12–14 There was one case of sterile endophthalmitis in our study. One study found only one case of acute endophthalmitis due to S. epidermidis in 1886 cases of TA-assisted PPV, suggesting that TA is not a high-risk factor for acute endophthalmitis.17 We reported three cases of steroid-induced glaucoma, which was controlled with antiglaucoma drops. Another study found antiglaucoma eye drops were required more frequently after TA-assisted PPV than after conventional PPV without dyes, but IOP was well controlled in both groups.18 ERM in our study was within the range of previous reports of 5–12%, although one study reported recurrence as high as 58% at 5-year follow-up.19,20

Chromovitrectomy

The use of dyes to improve the poor intra-operative visibility of the ERM and ILM is increasingly seen in practice. In addition to improving visualization, dyes can help with shortening the duration of surgery and can minimize retinal trauma during ERM removal. Each dye offers different advantages and risks.

ICG was utilized early in chromovitrectomy visualization due to intensity of staining and ease of ILM removal, but its use can be complicated by retinal toxicity including retinal pigment epithelial damage, optic nerve atrophy, visual field defects and decreased multifocal electroretinographic responses.3,7,8 Due to its packaging as a lyophilized powder, ICG will not dissolve in intraocular irrigation solution alone and requires dilution which can be tedious to both prepare and remove from the eye.4 Tb emerged as the alternative to ICG, but has also been associated with retinal toxicity and requires an air-fluid exchange before application.4,5 BBG was later introduced as the most promising alternative to ICG and Tb.4 These granules can easily be dissolved in intraocular irrigating solution alone and ILM staining patterns are similar to that of ICG solution with little possibility of light toxicity.5 Although rare, BBG has also been associated with macular toxicity in a few cases.6 Comparative studies have found BBG to be superior to TB and similar to ICG in terms of staining and ease of ILM removal.4,5

Interestingly, TA was the first FDA-approved agent for chromovitrectomy and despite its limited use in recent years, there is still a role for TA visualization in ERM surgery. TA has been used in PPV and found to have outcomes similar to conventional PPV and has been well documented for visualization.18,21,22 Unlike the dyes, TA can be utilized in visualization of the vitreous, ERM, and ILM. In vitreous visualization, the crystals become trapped in the vitreous gel with the suspension allowing for good contrast between the whitened vitreous and the post-hyaloid space. This allows ease of removal of vitreous and hyaloid membrane, which if left behind may provide scaffolding for fibrovascular proliferation and lead to a recurrent ERM or may provide a foundation of traction that can precipitate a macular hole or vitreomacular traction.23,24

Advantages of TA visualization compared to other agents include ease of use given its readiness for immediate use without need for dilution or preparation, no reported retinal toxicity in doses of 2 to 4mg, and no requirement for an air-fluid exchange.25 TA coats the surface of ERMs and ILMs and integrates into their superficial collagen fibrils compared to the dyes, which stain the membranes.24,26,27 This allows any remaining TA particles to be removed from the eye with aspiration or irrigation. TA is also less likely to make the view hazy if there is disbursement of the agent.

It is often injected following PPV to treat ME and inflammation in various retinal diseases and uveitis, which may offer an additional benefit in the treatment of postoperative CME following ERM removal. TA has also been shown to reduce post-operative inflammation and inhibit the postoperative breakdown of the blood-ocular barrier when utilized in conjunction with PPV.28,29 Microscopic granules left behind following aspiration of TA could contribute to improved outcomes in these patients.

The use of TA use does not come without disadvantages. TA is a steroid, which is known to be associated with cataract progression and intraocular pressure elevation. The coating ability of TA may limit the ability to discriminate residual ILM from ILM defects following ERM removal. A case series demonstrated incomplete removal of perifoveal ILM in 29% of cases undergoing ILM removal with TA and subsequent ICG to assess the status of the remaining ILM.30 The residual ILM can serve as a scaffold for recurrent ERM formation.31 There has been a move away from the use of TA in recent years with the argument that vital dyes more accurately stain the ERM and differentiate the ILM. This has not been our experience. The consistency of the ILM visualized with TA is clearly distinct from the ERM and an experienced surgeon with understanding of the retinal anatomy should have no difficulty differentiating.

Limitations

This retrospective study has several limitations. In addition to its retrospective nature, the study did not utilize a control arm and cannot state with certainty the advantages or disadvantages of TA visualization over other agents. Future studies that compare outcomes of ERM surgery with different visualization agents may be able to further elucidate the differences in outcomes intra-operatively and post-operatively.

Conclusion

Combination ILM forceps and TA visualization offers an affordable option for ERM removal with comparable or better outcomes than traditional methods of ERM surgery. We demonstrated that there is a reduced incidence of intraoperative complications with TA in PPV for ERM, which is consistent with prior studies.18,32 Complications at one year post-operatively were consistent with prior reports and we saw no further complications at three and five years post-operatively with statistically significant improvement in BCVA and CMT at all time points post-operatively.