| Literature DB >> 33230763 |
Azadeh Sarfallah1, Dmitry Temiakov2.
Abstract
In vitro assay based on a reconstituted mitochondrial transcription system serves as a method of choice to probe the functional importance of proteins and their structural motifs. Here we describe protocols for transcription assays designed to probe activity of the human mitochondrial RNA polymerase and the transcription initiation complex using RNA-DNA scaffold and synthetic promoter templates.Entities:
Keywords: Mitochondrial transcription; POLRTM; Promoter; RNAP; TFAM; TFB2M
Mesh：
Substances：
Year: 2021 PMID： 33230763 DOI： 10.1007/978-1-0716-0834-0_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745