Endophytic actinobacteria of a halophytic desert plant Pteropyrum olivieri: promising growth enhancers of sunflower.

In the present study, 40 actinobacterial isolates were obtained from the roots of a desert plant, Pteropyrum olivieri and tested for extracellular hydrolytic enzyme activities, hydrogen cyanide, and siderophore production. Based on these activities, three isolates designated UTMC 2482, UTMC 2483, and UTMC 3136 were selected with an aim of developing bio-fertilizing agent to improve the growth of sunflower plants under normal conditions. The selected isolates showed 98.2, 98.4, and 100% similarities in the 16S rRNA gene sequences to Streptomyces chromofuscus, Streptomyces ambofaciens, and Streptomyces gardneri, respectively. These isolates exhibited indole acetic acid production while UTMC 2483 was found to produce 1-aminocyclopropane-1-carboxylate deaminase, as well. Sunflower seeds soaked in the bacterial spore suspensions increased the tolerance of sunflower seedlings to the stresses of salinity and water deficiency up to 270 mM of NaCl and - 2Mpa of PEG6000, respectively. Under normal conditions, inoculation with individual isolates and their consortia enhanced the yield (plant length, weight, and flower diameter) and biochemical contents (i.e. chlorophyll, protein, and oil) up to 5.3, 1.7, and 2.4 times higher than that of un-inoculated plants, significantly (p < 0.05) in greenhouse and field experiments. This is the first study demonstrating that endophytic actinobacteria from the desert plant, P. olivieri, have profound bio-fertilizing effects on the growth of sunflower. © King Abdulaziz City for Science and Technology 2020.