| Literature DB >> 33164145 |
Rebecca Sprague1, Karolyne Warny2, Nira Pollock3,4, Kaitlyn Daugherty2, Qianyun Lin2, Hua Xu2, Christine Cuddemi2, Caitlin Barrett2, Xinhua Chen2, Alice Banz5, Aude Lantz5, Kevin W Garey6, Anne J Gonzales-Luna6, Carolyn D Alonso3, Javier A Villafuerte Galvez2, Ciarán P Kelly2.
Abstract
Clostridioides difficile infection (CDI) is caused by Toxins A and B, secreted from pathogenic strains of C. difficle. This infection can vary greatly in symptom severity and in clinical presentation. Current assays used to diagnose CDI may lack the required sensitivity to detect the exotoxins circulating in blood. The ultrasensitive single molecule array (Simoa) assay was modified to separately detect toxin A and toxin B in serum with a limit of detection at the low picogram level. When applied to a diverse cohort, Simoa was unable to detect toxins A or B in serum from patients with CDI, including many classified as having severe disease. The detection of toxin may be limited by the inference of antitoxin antibodies circulating in serum. This result does not support the hypothesis that toxemia occurs in C. difficile infection, conflicting with the findings of other published reports.Entities:
Keywords: Antibiotic-associated diarrhea; Clostridioides difficile; Colitis; Infectious diarrhea; Nosocomial infection; Simoa ultrasensitive toxin assay
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Year: 2020 PMID： 33164145 PMCID： PMC8105423 DOI： 10.1007/s10620-020-06683-8
Source DB: PubMed Journal: Dig Dis Sci ISSN： 0163-2116 Impact factor: 3.487