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Literature DB >> 33159590

qPCR assay for the detection of pseudocowpox virus.

Gabriel Augusto de Oliveira Lopes^1,2, Luciana Rabello Ferreira², Giliane de Souza Trindade³, Antônio Augusto Fonseca², Jenner Karlisson Pimenta Dos Reis⁴.

Abstract

Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals.

Entities: Disease Species

Year: 2020 PMID： 33159590 DOI： 10.1007/s00705-020-04872-4

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

15 in total

1. The genome of pseudocowpoxvirus: comparison of a reindeer isolate and a reference strain.

Authors: Maria Hautaniemi; Norihito Ueda; Jarno Tuimala; Andrew A Mercer; Juhani Lahdenperä; Colin J McInnes
Journal: J Gen Virol Date: 2010-01-27 Impact factor: 3.891

2. Detection of poxvirus in cattle associated with human cases in the State of Rio de Janeiro: preliminary report.

Authors: H G Schatzmayr; E R Lemos; C Mazur; A Schubach; S Majerowicz; T Rozental; T M Schubach; M C Bustamante; O M Barth
Journal: Mem Inst Oswaldo Cruz Date: 2000 Sep-Oct Impact factor: 2.743

Review 3. Real-time PCR detection chemistry.

Authors: E Navarro; G Serrano-Heras; M J Castaño; J Solera
Journal: Clin Chim Acta Date: 2014-10-22 Impact factor: 3.786

4. Persistent pseudocowpox virus infection of the skin of a foot in a cat.

Authors: R A Fairley; A A Mercer; C I Copland; S M Craig; P A Heslip
Journal: N Z Vet J Date: 2013-02-28 Impact factor: 1.628

5. Detection and diagnosis of parapoxvirus by the polymerase chain reaction.

Authors: Y Inoshima; A Morooka; H Sentsui
Journal: J Virol Methods Date: 2000-02 Impact factor: 2.014

6. Human Vaccinia virus and Pseudocowpox virus co-infection: clinical description and phylogenetic characterization.

Authors: Jônatas S Abrahão; André T Silva-Fernandes; Felipe L Assis; Maria I Guedes; Betânia P Drumond; Juliana A Leite; Luiz F L Coelho; Filippo Turrini; Flávio G Fonseca; Zélia I P Lobato; Marieta Madureira; Paulo C Ferreira; Cláudio A Bonjardim; Giliane S Trindade; Erna G Kroon
Journal: J Clin Virol Date: 2010-03-05 Impact factor: 3.168

7. Detection of pseudocowpox virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016.

Authors: Mateus Laguardia-Nascimento; Ana Paula Ferreira de Oliveira; Fernanda Rodas Pires Fernandes; Anselmo Vasconcelos Rivetti; Marcelo Fernandes Camargos; Antônio Augusto Fonseca Júnior
Journal: Vet Q Date: 2016-11-08 Impact factor: 3.320

8. Occurrence of Pseudocowpox virus associated to Bovine viral diarrhea virus-1, Brazilian Amazon.

Authors: Pedro A Alves; Poliana O Figueiredo; Cairo H S de Oliveira; José D Barbosa; Danillo H S Lima; Henrique A Bomjardim; Natália S Silva; Karinny F Campos; Carlos Magno C Oliveira; Edel Figueiredo Barbosa-Stancioli; Jônatas S Abrahão; Erna G Kroon; Giliane de Souza Trindade
Journal: Comp Immunol Microbiol Infect Dis Date: 2016-09-29 Impact factor: 2.268