| Literature DB >> 32994364 |
Ellen Shrock1,2, Eric Fujimura1,2,3, Tomasz Kula1,2, Richard T Timms1,2, I-Hsiu Lee4, Yumei Leng1,2, Matthew L Robinson5, Brandon M Sie1,2, Mamie Z Li1,2, Yuezhou Chen6,7, Jennifer Logue8, Adam Zuiani6,7, Denise McCulloch8, Felipe J N Lelis6,7, Stephanie Henson9, Daniel R Monaco9, Meghan Travers6,7, Shaghayegh Habibi6,7, William A Clarke10, Patrizio Caturegli11, Oliver Laeyendecker5,12, Alicja Piechocka-Trocha7,13, Jonathan Z Li7,14, Ashok Khatri15, Helen Y Chu8, Alexandra-Chloé Villani16, Kyle Kays17, Marcia B Goldberg18, Nir Hacohen19, Michael R Filbin17, Xu G Yu7,14,20,21, Bruce D Walker7,13,22, Duane R Wesemann6,7, H Benjamin Larman9, James A Lederer23, Stephen J Elledge24,2,7.
Abstract
Understanding humoral responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for improving diagnostics, therapeutics, and vaccines. Deep serological profiling of 232 coronavirus disease 2019 (COVID-19) patients and 190 pre-COVID-19 era controls using VirScan revealed more than 800 epitopes in the SARS-CoV-2 proteome, including 10 epitopes likely recognized by neutralizing antibodies. Preexisting antibodies in controls recognized SARS-CoV-2 ORF1, whereas only COVID-19 patient antibodies primarily recognized spike protein and nucleoprotein. A machine learning model trained on VirScan data predicted SARS-CoV-2 exposure history with 99% sensitivity and 98% specificity; a rapid Luminex-based diagnostic was developed from the most discriminatory SARS-CoV-2 peptides. Individuals with more severe COVID-19 exhibited stronger and broader SARS-CoV-2 responses, weaker antibody responses to prior infections, and higher incidence of cytomegalovirus and herpes simplex virus 1, possibly influenced by demographic covariates. Among hospitalized patients, males produce stronger SARS-CoV-2 antibody responses than females.Entities:
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Year: 2020 PMID： 32994364 PMCID： PMC7857405 DOI： 10.1126/science.abd4250
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 63.714