Literature DB >> 33155413

Mesenchymal stroma/stem-like cells of GARP knockdown inhibits cell proliferation and invasion of mouse colon cancer cells (MC38) through exosomes.

Haizhou Xing1, Chunyan Liang1, Xintong Xu1, Hui Sun1, Xiaojun Ma1, Zhongxing Jiang1.   

Abstract

Mesenchymal stroma/stem-like cells (MSCs) have antitumour activity, and MSC-derived exosomes play a role in the growth, metastasis and invasion of tumour cells. Additionally, glycoprotein A repetition predominant (GARP) promotes oncogenesis in breast cancer. Therefore, GARP is speculated to be a target gene for cancer therapy. We aimed to explore the therapy role of MSC-derived exosomes targeting GARP in mouse colon cancer cell MC38. We successfully established a GARP knockdown system using three kinds of siRNA-GARP in MSC cells. Exosomes were isolated from MSC and siGARP-MSC cells, and verified by the exosome surface protein markers CD9, CD63 and CD81. GARP expression was significantly decreased in siGARP-MSC exosomes compared with that of MSC exosomes. We found that siGARP-MSC exosomes inhibited cell proliferation, migration and invasion of MC38 cells, using CCK-8, colony formation, wound-healing and Transwell invasion assays. Furthermore, siGARP-MSC exosomes impeded IL-6 secretion and partly inactivated JAK1/STAT3 pathway, measured using ELISA and RT-qPCR. In conclusion, MSC-derived exosomes targeting GARP are a potential strategy for cancer therapy.
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

Entities:  

Keywords:  GARP; IL-6; JAK1/STAT3 pathway; MC38 cells; MSC; cancer therapy; cell proliferation; exosome; invasion; migration

Mesh：

Substances：

Year:  2020        PMID： 33155413      PMCID： PMC7753840          DOI： 10.1111/jcmm.16008

Source DB:  PubMed          Journal:  J Cell Mol Med        ISSN： 1582-1838            Impact factor:   5.295


  24 in total

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7.  Mesenchymal stroma/stem-like cells of GARP knockdown inhibits cell proliferation and invasion of mouse colon cancer cells (MC38) through exosomes.

Authors:  Haizhou Xing; Chunyan Liang; Xintong Xu; Hui Sun; Xiaojun Ma; Zhongxing Jiang
Journal:  J Cell Mol Med       Date:  2020-11-05       Impact factor: 5.295
  7 in total

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