Huijuan Jin1, Xiaojie Gao1, Li Xiao1, Huimin He1, Sinan Cheng1, Caixia Zhang1, Yifan Hou1, Fengying Song1, Xiaorong Su1, Qian Gao1, Zheng Lu1, Ruina Yang1, Xigui Song1, Jin Yang2, Wei Duan3, Yingchun Hou4. 1. Laboratory of Tumor Molecular and Cellular Biology, College of Life Sciences, Shaanxi Normal University, 620 West Chang'an Avenue, Xi'an, 710119, Shaanxi, China. 2. The College of Life, Northwest University, Xi'an, 710069, Shaanxi, China. 3. School of Medicine, Deakin University, Waurn Ponds, VIC, 3216, Australia. 4. Laboratory of Tumor Molecular and Cellular Biology, College of Life Sciences, Shaanxi Normal University, 620 West Chang'an Avenue, Xi'an, 710119, Shaanxi, China. ychhou@snnu.edu.cn.
Abstract
OBJECTIVES: Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specifically binding molecule or fragment as early clinical indicators. RESULTS: By a phage-displayed peptide library, a 12-mer peptide, CSB1 was screened out using MCF-7 cells as the target. The consequently results under immunofluorescence and laser scanning confocal microscope (LSCM) indicated that CSB1 bound MCF-7 cells and breast cancer tissues specifically and sensitively with high affinity. Bioinformatics analysis suggested that the peptide CSB1 targets the 5-Lipoxygenase-Activating Protein (FLAP), which has been implicated in breast cancer progression and prognosis. CONCLUSIONS: The peptide, CSB1 is of the potential as a candidate to be used for developing the new approaches of molecular imaging detection and targeting chemotherapy of breast cancer in the future.
OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specifically binding molecule or fragment as early clinical indicators. RESULTS: By a phage-displayed peptide library, a 12-mer peptide, CSB1 was screened out using MCF-7 cells as the target. The consequently results under immunofluorescence and laser scanning confocal microscope (LSCM) indicated that CSB1 bound MCF-7 cells and breast cancer tissues specifically and sensitively with high affinity. Bioinformatics analysis suggested that the peptide CSB1 targets the 5-Lipoxygenase-Activating Protein (FLAP), which has been implicated in breast cancer progression and prognosis. CONCLUSIONS: The peptide, CSB1 is of the potential as a candidate to be used for developing the new approaches of molecular imaging detection and targeting chemotherapy of breast cancer in the future.
Entities:
Keywords:
Biopanning; Breast cancer; Peptide library; Targeting peptide
Authors: W J Kort; A M Bijma; J J van Dam; A C van der Ham; J M Hekking; H F van der Ingh; W S Meijer; M G van Wilgenburg; F J Zijlstra Journal: Prostaglandins Leukot Essent Fatty Acids Date: 1992-04 Impact factor: 4.006
Authors: Cornelia Halin; Verena Gafner; Maria Elena Villani; Laura Borsi; Alexander Berndt; Hartwig Kosmehl; Luciano Zardi; Dario Neri Journal: Cancer Res Date: 2003-06-15 Impact factor: 12.701