Literature DB >> 331262

A strong ethidium binding site in the acceptor stem of most or all transfer RNAs.

B D Wells, C R Cantor.   

Abstract

E. coli unfractionated tRNA and tRNA phe both contain a single strong ethidium binding site. Singlet-singlet energy transfer has been used to measure the distance between this site and dansyl hydrazine covalently attached to the 3' end of the tRNAs. The distance obtained is between 33 and 40 A for both samples. This is completely consistent with results from earlier NMR studies which placed the single, strong ethidium binding site of yeast tRNAphe between base pairs 6 and 7 on the aminoacyl stem. From the known tertiary structure of tRNAphe it is possible to rationalize the unusual affinity of this site and its likely existence in all tRNAs.

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Year:  1977        PMID： 331262      PMCID： PMC343780          DOI： 10.1093/nar/4.5.1667

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Effect of 50 S subunit proteins L5, L18 and L25 on the fluorescence of 5 S RNA-bound ethidium bromide.

Authors:  J Fuenteun; R Monier; R Garrett; M Le Bret; J B Le Pecq
Journal:  J Mol Biol       Date:  1975-04-25       Impact factor: 5.469

2.  Fluorescence studies on the 30 S ribosome assembly process.

Authors:  A Bollen; A Herzog; A Favre; J Thibault; F Gros
Journal:  FEBS Lett       Date:  1970-11-09       Impact factor: 4.124

3.  Organization of DNA in chromatin.

Authors:  H M Sobell; C C Tsai; S G Gilbert; S C Jain; T D Sakore
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

4.  The binding of ethidium bromide to different conformations of tRNA. Unfolding of tertiary structure.

Authors:  C Urbanke; R Römer; G Maass
Journal:  Eur J Biochem       Date:  1973-03-15

5.  The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors:  W Bauer; J Vinograd
Journal:  J Mol Biol       Date:  1968-04-14       Impact factor: 5.469

6.  Studies of the binding of ethidium bromide to transfer ribonucleic acid: absorption, fluorescence, ultracentrifugation and kinetic investigations.

Authors:  R Bittman
Journal:  J Mol Biol       Date:  1969-12-14       Impact factor: 5.469

7.  Fluorescence decay time characteristics of the complex between ethidium bromide and nucleic acids.

Authors:  V W Burns
Journal:  Arch Biochem Biophys       Date:  1969-09       Impact factor: 4.013

8.  Conformational studies on transfer ribonucleic acid. Fluorescence lifetime and nanosecond depolarization measurements on bound ethidium bromidee.

Authors:  T Tao; J H Nelson; C R Cantor
Journal:  Biochemistry       Date:  1970-09-01       Impact factor: 3.162

9.  Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA.

Authors:  P Wahl; J Paoletti; J B Le Pecq
Journal:  Proc Natl Acad Sci U S A       Date:  1970-02       Impact factor: 11.205

10.  Structural domains of transfer RNA molecules.

Authors:  G J Quigley; A Rich
Journal:  Science       Date:  1976-11-19       Impact factor: 47.728
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  9 in total

1.  Efficient RNA 5'-adenylation by T4 DNA ligase to facilitate practical applications.

Authors:  Yangming Wang; Scott K Silverman
Journal:  RNA       Date:  2006-04-17       Impact factor: 4.942

2.  Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids.

Authors:  P H Bolton; D R Kearns
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

3.  Localization of the major ethidium bromide binding site on tRNA.

Authors:  W C Chu; J C Liu; J Horowitz
Journal:  Nucleic Acids Res       Date:  1997-10-01       Impact factor: 16.971

4.  Ribosome binding by tRNAs with fluorescent labeled 3' termini.

Authors:  B D Wells; C R Cantor
Journal:  Nucleic Acids Res       Date:  1980-07-25       Impact factor: 16.971

5.  Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena: inhibition by methidiumpropyl.EDTA and localization of the major dye binding sites.

Authors:  N K Tanner; T R Cech
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

6.  Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena: inhibition by intercalating dyes.

Authors:  N K Tanner; T R Cech
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

7.  Nonintercalative binding of ethidium bromide to nucleic acids: crystal structure of an ethidium--tRNA molecular complex.

Authors:  M Liebman; J Rubin; M Sundaralingam
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

8.  Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.

Authors:  Marina Toompuu; Tea Tuomela; Pia Laine; Lars Paulin; Eric Dufour; Howard T Jacobs
Journal:  Nucleic Acids Res       Date:  2018-06-01       Impact factor: 16.971

9.  Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.

Authors:  K Ainger; D Avossa; F Morgan; S J Hill; C Barry; E Barbarese; J H Carson
Journal:  J Cell Biol       Date:  1993-10       Impact factor: 10.539
  9 in total

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