Silvana Carnevale1,2, Jorge Bruno Malandrini3, María Laura Pantano1, Mirna Sawicki4, Laura Kamenetzky5, Claudia Cecilia Soria3, Jorge Néstor Velásquez6. 1. Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, ANLIS "Dr. Carlos G. Malbrán", Avenida Vélez Sarsfield 563,, CP 1281, Ciudad de Buenos Aires, Argentina. 2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad de Buenos Aires, Argentina. 3. Facultad de Ciencias de la Salud, Universidad Nacional de Catamarca, Maestro Quiroga 1ra. Cuadra, CP 4700, San Fernando del Valle de Catamarca, Catamarca, Argentina. 4. Hospital de Infecciosas "Dr. Francisco Javier Muñiz". Uspallata 2272, CP 1282, Ciudad de Buenos Aires, Argentina. 5. Instituto de Microbiología y Parasitología Médica (IMPaM), UBA-CONICET, Paraguay 2155 piso 13, CP 1121, Ciudad de Buenos Aires, Argentina. 6. Hospital de Infecciosas "Dr. Francisco Javier Muñiz". Uspallata 2272, CP 1282, Ciudad de Buenos Aires, Argentina. jorgeysilvana@speedy.com.ar.
Abstract
PURPOSE: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. METHODS: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. RESULTS: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. CONCLUSION: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.
PURPOSE:Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques. Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate humanfascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals. METHODS: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp. RESULTS: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases. CONCLUSION: This is the first report employing an approach based on the combination of methods for the evaluation of humanfascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.
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Authors: S Carnevale; M I Rodríguez; G Santillán; J H Labbé; M G Cabrera; E J Bellegarde; J N Velásquez; J E Trgovcic; E A Guarnera Journal: Clin Diagn Lab Immunol Date: 2001-01
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